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    PUS1 pseudouridine synthase PUS1 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 855889, updated on 2-Nov-2024

    Summary

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    Official Symbol
    PUS1
    Official Full Name
    pseudouridine synthase PUS1
    Primary source
    SGD:S000006133
    Locus tag
    YPL212C
    See related
    AllianceGenome:SGD:S000006133; FungiDB:YPL212C; VEuPathDB:YPL212C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables pseudouridine synthase activity. Involved in mRNA pseudouridine synthesis; snRNA pseudouridine synthesis; and tRNA pseudouridine synthesis. Located in nucleus. Human ortholog(s) of this gene implicated in myopathy, lactic acidosis, and sideroblastic anemia 1. Orthologous to human PUS1 (pseudouridine synthase 1). [provided by Alliance of Genome Resources, Nov 2024]
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    Genomic context

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    See PUS1 in Genome Data Viewer
    Location:
    chromosome: XVI
    Exon count:
    1
    Sequence:
    Chromosome: XVI; NC_001148.4 (151515..153149, complement)

    Chromosome XVI - NC_001148.4Genomic Context describing neighboring genes Neighboring gene bifunctional hydroxyethylthiazole kinase/thiamine-phosphate diphosphorylase Neighboring gene U2 snRNP complex subunit LEA1 Neighboring gene ribosome biosynthesis protein NIP7 Neighboring gene signal recognition particle subunit SRP72

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001148.4

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Transfer RNA-pseudouridine synthetase Pus1 of Saccharomyces cerevisiae contains one atom of zinc essential for its native conformation and tRNA recognition. Arluison V, et al. Biochemistry, 1998 May 19. PMID 9585540
    2. The yeast tRNA:pseudouridine synthase Pus1p displays a multisite substrate specificity. Motorin Y, et al. RNA, 1998 Jul. PMID 9671058, Free PMC Article
    3. Pseudouridine mapping in the Saccharomyces cerevisiae spliceosomal U small nuclear RNAs (snRNAs) reveals that pseudouridine synthase pus1p exhibits a dual substrate specificity for U2 snRNA and tRNA. Massenet S, et al. Mol Cell Biol, 1999 Mar. PMID 10022901, Free PMC Article
    4. Pseudouridine synthetase Pus1 of Saccharomyces cerevisiae: kinetic characterisation, tRNA structural requirement and real-time analysis of its complex with tRNA. Arluison V, et al. J Mol Biol, 1999 Jun 11. PMID 10356324
    5. RNA:pseudouridine synthetase Pus1 from Saccharomyces cerevisiae: oligomerization property and stoichiometry of the complex with yeast tRNA(Phe). Arluison V, et al. Biochimie, 1999 Jul. PMID 10492022

    See all (67) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Impact of Pus1 Pseudouridine Synthase on Specific Decoding Events in Saccharomyces cerevisiae.
      Title: Impact of Pus1 Pseudouridine Synthase on Specific Decoding Events in Saccharomyces cerevisiae.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables RNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables isomerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables mRNA binding HDA PubMed 
    enables pseudouridine synthase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables pseudouridine synthase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables pseudouridine synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables snRNA pseudouridine synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables tRNA pseudouridine synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables zinc ion binding RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in mRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in mRNA pseudouridine synthesis IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in mRNA pseudouridine synthesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in snRNA pseudouridine synthesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in tRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tRNA pseudouridine synthesis IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in tRNA pseudouridine synthesis IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in tRNA pseudouridine synthesis IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in intracellular membrane-bounded organelle IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    pseudouridine synthase PUS1
    NP_015112.1
    • tRNA:pseudouridine synthase; introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; also acts on U2 snRNA; also pseudouridylates some mRNAs, and pseudouridylation level varies with growth phase; nuclear protein that appears to be involved in tRNA export; PUS1 has a paralog, PUS2, that arose from the whole genome duplication

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001148.4 Reference assembly

      Range
      151515..153149 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001184026.1 → NP_015112.1  TPA: pseudouridine synthase PUS1 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_015112.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6W3F8, Q12211
      UniProtKB/TrEMBL
      A6ZW43, B3LKL8, C7GN39, C8ZIG7, N1NWM6
      Conserved Domains (1) summary
      cd02568
      Location:79 → 407
      PseudoU_synth_PUS1_PUS2; Pseudouridine synthase, PUS1/ PUS2 like
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q12211.1

    Gene LinkOut

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