TPK3 cAMP-dependent protein kinase catalytic subunit TPK3 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: TPK3
Official Full Name: cAMP-dependent protein kinase catalytic subunit TPK3
Primary source: SGD:S000001649
Locus tag: YKL166C
See related: AllianceGenome:SGD:S000001649; FungiDB:YKL166C; VEuPathDB:YKL166C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables cAMP-dependent protein kinase activity. Involved in several processes, including intracellular signal transduction; negative regulation of cytoplasmic mRNA processing body assembly; and negative regulation of cytoplasmic translation. Located in P-body; cytoplasmic stress granule; and nucleus. Part of cAMP-dependent protein kinase complex. Human ortholog(s) of this gene implicated in adrenal cortical adenoma; congestive heart failure; fibrolamellar carcinoma; platelet-type bleeding disorder 19; and primary pigmented nodular adrenocortical disease 4. Orthologous to several human genes including PRKACA (protein kinase cAMP-activated catalytic subunit alpha); PRKACB (protein kinase cAMP-activated catalytic subunit beta); and PRKACG (protein kinase cAMP-activated catalytic subunit gamma). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See TPK3 in Genome Data Viewer

Location:: chromosome: XI

Exon count:: 1

Sequence:: Chromosome: XI; NC_001143.9 (134509..135705, complement)

Chromosome XI - NC_001143.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001143.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Isoform-specific sequestration of protein kinase A fine-tunes intracellular signaling during heat stress.
Title: Isoform-specific sequestration of protein kinase A fine-tunes intracellular signaling during heat stress.
Data indicate that Rgt1 and tpk1/2/3 (cAMP-dependent protein kinases) participate in the control of SUC2 expression.
Title: The repressor Rgt1 and the cAMP-dependent protein kinases control the expression of the SUC2 gene in Saccharomyces cerevisiae.
Tpk2 and Tpk3 are associated with translation initiation factors Pab1 and Rps3 in exponentially growing cells.
Title: PKA isoforms coordinate mRNA fate during nutrient starvation.
Trehalase activation is associated with phosphorylation of typical PKA sites.
Title: In vivo phosphorylation of Ser21 and Ser83 during nutrient-induced activation of the yeast protein kinase A (PKA) target trehalase.
Tpk3 is poorly phosphorylated on its activation loop site due to its weak interaction with PKB (protein kinase B)-activating kinase homologue Pkh1.
Title: The activation loop of PKA catalytic isoforms is differentially phosphorylated by Pkh protein kinases in Saccharomyces cerevisiae.
Data show that the Ras2p localization is regulated by PKA, and that PKA down-regulates Ras2p activity and the protein association between Cdc25p and Ras2-GTP, which is due to a reduced Ras2-GEF Cdc25p activity.
Title: The membrane localization of Ras2p and the association between Cdc25p and Ras2-GTP are regulated by protein kinase A (PKA) in the yeast Saccharomyces cerevisiae.
Data show that Atg1, PKA directly phosphorylates Atg13 in a Tor-dependent manner.
Title: The Tor and PKA signaling pathways independently target the Atg1/Atg13 protein kinase complex to control autophagy.
Tor and cyclic AMP-protein kinase A regulate expression of genes required for cell growth
Title: Tor and cyclic AMP-protein kinase A: two parallel pathways regulating expression of genes required for cell growth.
Tpk3p is specifically involved in the regulation of cellular mitochondrial content when energy demand decreases.
Title: The yeast cAMP protein kinase Tpk3p is involved in the regulation of mitochondrial enzymatic content during growth.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables cAMP-dependent protein kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables cAMP-dependent protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables cAMP-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables cAMP-dependent protein kinase activity	IGI Inferred from Genetic Interaction more info	PubMed
enables cAMP-dependent protein kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein kinase activity	HDA more info	PubMed
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in G1 to G0 transition	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in Ras protein signal transduction	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitochondrion organization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitochondrion organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cytoplasmic mRNA processing body assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of cytoplasmic translation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein kinase A signaling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in signal transduction	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in P-body	IDA Inferred from Direct Assay more info	PubMed
part_of cAMP-dependent protein kinase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of cAMP-dependent protein kinase complex	IGI Inferred from Genetic Interaction more info	PubMed
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasmic stress granule	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: cAMP-dependent protein kinase catalytic subunit TPK3

NP_012755.1

EC 2.7.11.11

cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk2p; localizes to P-bodies during stationary phase; TPK3 has a paralog, TPK1, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001143.9 Reference assembly

Range

134509..135705 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179732.1 → NP_012755.1 TPA: cAMP-dependent protein kinase catalytic subunit TPK3 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012755.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VX33, P05986

UniProtKB/TrEMBL

A6ZZF8, B3LQT8, C7GJ41, C8ZC07, G2WHM5, N1NZN3

Conserved Domains (1) summary

cd05580
Location:86 → 376

STKc_PKA_like; Catalytic subunit of the Serine/Threonine Kinases, cAMP-dependent protein kinases