UBA4 Uba4p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: UBA4
Official Full Name: Uba4p
Primary source: SGD:S000001153
Locus tag: YHR111W
See related: AllianceGenome:SGD:S000001153; FungiDB:YHR111W; VEuPathDB:YHR111W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: YHR1
Summary: Enables AMPylase activity; URM1 activating enzyme activity; and thiosulfate sulfurtransferase activity. Involved in several processes, including invasive growth in response to glucose limitation; protein urmylation; and tRNA wobble position uridine thiolation. Located in cytoplasm. Orthologous to human MOCS3 (molybdenum cofactor synthesis 3). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See UBA4 in Genome Data Viewer

Location:: chromosome: VIII

Exon count:: 1

Sequence:: Chromosome: VIII; NC_001140.6 (333072..334394)

Chromosome VIII - NC_001140.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001140.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A critical thioester linkage between Urm1 and Uba4 residue Cys225.This linkage is indispensable for the Urm1 intramolecular transfer between the two domains of Uba4 and it is thus, essential for tRNA thiolation .
Title: The Uba4 domain interplay is mediated via a thioester that is critical for tRNA thiolation through Urm1 thiocarboxylation.
ubiquitin-like Urm1.Uba4 systems are conserved and exchangeable between human and yeast cells
Title: Urmylation and tRNA thiolation functions of ubiquitin-like Uba4·Urm1 systems are conserved from yeast to man.
ubiquitin-like protein Urm1 and ubiquitin-activating enzyme-like protein Uba4, as well as Tuc1 and Tuc2, were strictly required for tRNA thio-modification
Title: Thio-modification of yeast cytosolic tRNA requires a ubiquitin-related system that resembles bacterial sulfur transfer systems.
The Uba4-Urm1 system represents the evolutionary link between protein conjugation and protein modification by sulfur carrier proteins.
Title: The sulfurtransferase activity of Uba4 presents a link between ubiquitin-like protein conjugation and activation of sulfur carrier proteins.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables AMPylase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables URM1 activating enzyme activity	IBA Inferred from Biological aspect of Ancestor more info
enables URM1 activating enzyme activity	IDA Inferred from Direct Assay more info	PubMed
enables adenylyltransferase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotidyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables nucleotidyltransferase activity	IEA Inferred from Electronic Annotation more info
enables sulfurtransferase activity	IEA Inferred from Electronic Annotation more info
enables sulfurtransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables thiosulfate sulfurtransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables thiosulfate sulfurtransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin-like modifier activating enzyme activity	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in cell budding	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to oxidative stress	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in invasive growth in response to glucose limitation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in invasive growth in response to glucose limitation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in macromolecule modification	IEA Inferred from Electronic Annotation more info
involved_in protein urmylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein urmylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein urmylation	IEA Inferred from Electronic Annotation more info
involved_in protein urmylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of pseudohyphal growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in tRNA processing	IEA Inferred from Electronic Annotation more info
involved_in tRNA thio-modification	IEA Inferred from Electronic Annotation more info
involved_in tRNA wobble position uridine thiolation	IBA Inferred from Biological aspect of Ancestor more info
involved_in tRNA wobble position uridine thiolation	IEA Inferred from Electronic Annotation more info
involved_in tRNA wobble position uridine thiolation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in tRNA wobble uridine modification	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Uba4p

NP_011979.1

E1-like protein that activates Urm1p before urmylation; also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001140.6 Reference assembly

Range

333072..334394

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179241.1 → NP_011979.1 TPA: Uba4p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011979.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A6ZT19, B3LSM6, B5VK45, D3DL61, P38820

UniProtKB/TrEMBL

C7GLZ7, C8Z9Q4, G2WFI5, N1P4N4

Conserved Domains (2) summary

cd00757
Location:46 → 279

ThiF_MoeB_HesA_family; ThiF_MoeB_HesA. Family of E1-like enzymes involved in molybdopterin and thiamine biosynthesis family. The common reaction mechanism catalyzed by MoeB and ThiF, like other E1 enzymes, begins with a nucleophilic attack of the C-terminal carboxylate of MoaD ...

cd01526
Location:316 → 440

RHOD_ThiF; Member of the Rhodanese Homology Domain superfamily. This CD includes several putative molybdopterin synthase sulfurylases including the molybdenum cofactor biosynthetic protein (CnxF) of Aspergillus nidulans and the molybdenum cofactor synthesis protein ...