MSH2 mismatch repair ATPase MSH2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: MSH2
Official Full Name: mismatch repair ATPase MSH2
Primary source: SGD:S000005450
Locus tag: YOL090W
See related: AllianceGenome:SGD:S000005450; FungiDB:YOL090W; VEuPathDB:YOL090W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: PMS5
Summary: Enables ATP binding activity; ATP hydrolysis activity; and DNA secondary structure binding activity. Contributes to DNA binding activity. Involved in DNA metabolic process and silent mating-type cassette heterochromatin formation. Located in nuclear chromosome. Part of MutSalpha complex and MutSbeta complex. Used to study Lynch syndrome (multiple); cancer (multiple); and ovarian disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including Lynch syndrome (multiple); gastrointestinal system cancer (multiple); lung cancer (multiple); mismatch repair cancer syndrome; and transitional cell carcinoma. Orthologous to human MSH2 (mutS homolog 2). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See MSH2 in Genome Data Viewer

Location:: chromosome: XV

Exon count:: 1

Sequence:: Chromosome: XV; NC_001147.6 (147382..150276)

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Elevated MSH2 MSH3 expression interferes with DNA metabolism in vivo.
Title: Elevated MSH2 MSH3 expression interferes with DNA metabolism in vivo.
MutSalpha deficiency increases tolerance to DNA damage in yeast lacking postreplication repair.
Title: MutSα deficiency increases tolerance to DNA damage in yeast lacking postreplication repair.
Results provide evidence that the MSH2 gene encoding one of the crucial components of the mismatch repair, is required for transcriptional repression at silent mating-type loci and telomeres.
Title: Yeast mismatch repair components are required for stable inheritance of gene silencing.
Msh2-Msh6 Recognizes Mismatched DNA Base Pairs with Different Affinities. Msh2-Msh6 Binding Affects Mismatched Base Pair Dynamics.
Title: Mismatch Recognition by Saccharomyces cerevisiae Msh2-Msh6: Role of Structure and Dynamics.
The mutant Msh2-Msh6 complexes exhibited normal mispair recognition and were proficient at recruiting the MMR endonuclease Mlh1-Pms1 to mispaired DNA.
Title: The properties of Msh2-Msh6 ATP binding mutants suggest a signal amplification mechanism in DNA mismatch repair.
MSH2 overexpression affected the integrity of the DNA replication fork, causing genome instability phenotypes.
Title: Genomic Instability Promoted by Overexpression of Mismatch Repair Factors in Yeast: A Model for Understanding Cancer Progression.
Msh2-Msh3 hops over nucleosomes and other protein roadblocks, but maintains sufficient contact with DNA to recognize a single lesion. Msh2-Msh6 slides without hopping and is largely blocked by protein roadblocks.
Title: Dynamic DNA binding licenses a repair factor to bypass roadblocks in search of DNA lesions.
By detecting Pol2 and Msh2 dynamics within the same strain, we established that the mismatch recognition complex binds origins and spreads to adjacent regions with the replisome.
Title: The Eukaryotic Mismatch Recognition Complexes Track with the Replisome during DNA Synthesis.
Data suggest that bot hATP and structure-specific repair substrates cooperate to direct Msh2-Msh3-mediated repair and provide an explanation for the msh3Y942A separation-of-function phenotype.
Title: ATP binding and hydrolysis by Saccharomyces cerevisiae Msh2-Msh3 are differentially modulated by mismatch and double-strand break repair DNA substrates.
Msh2-Msh6 localizes PCNA to repair sites after mispair recognition.
Title: PCNA and Msh2-Msh6 activate an Mlh1-Pms1 endonuclease pathway required for Exo1-independent mismatch repair.

Submit: New GeneRIF Correction See all GeneRIFs (28)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP-dependent DNA damage sensor activity	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
contributes_to DNA insertion or deletion binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to Y-form DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables double-strand/single-strand DNA junction binding	IDA Inferred from Direct Assay more info	PubMed
enables four-way junction DNA binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to guanine/thymine mispair binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to heteroduplex DNA loop binding	IDA Inferred from Direct Assay more info	PubMed
enables mismatched DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables mismatched DNA binding	IEA Inferred from Electronic Annotation more info
contributes_to single base insertion or deletion binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to single base insertion or deletion binding	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA metabolic process	IEA Inferred from Electronic Annotation more info
involved_in DNA recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in interstrand cross-link repair	IGI Inferred from Genetic Interaction more info	PubMed
involved_in meiotic gene conversion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic mismatch repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mismatch repair	IBA Inferred from Biological aspect of Ancestor more info
involved_in mismatch repair	IEA Inferred from Electronic Annotation more info
involved_in mismatch repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in removal of nonhomologous ends	IGI Inferred from Genetic Interaction more info	PubMed
involved_in removal of nonhomologous ends	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in replication fork arrest	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in silent mating-type cassette heterochromatin formation	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
part_of MutSalpha complex	IBA Inferred from Biological aspect of Ancestor more info
part_of MutSalpha complex	IPI Inferred from Physical Interaction more info	PubMed
part_of MutSbeta complex	IPI Inferred from Physical Interaction more info	PubMed
located_in nuclear chromosome	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: mismatch repair ATPase MSH2

NP_014551.1

Protein that binds to DNA mismatches; forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP; required for silencing at the silent mating-type loci and telomeres

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6 Reference assembly

Range

147382..150276

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183344.1 → NP_014551.1 TPA: mismatch repair ATPase MSH2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014551.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W1X8, P25847, Q12423

UniProtKB/TrEMBL

B3LIX3, N1P404

Conserved Domains (1) summary

COG0249
Location:11 → 929

MutS; DNA mismatch repair ATPase MutS [Replication, recombination and repair]