SEC14 phosphatidylinositol/phosphatidylcholine transfer protein SEC14 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: SEC14
Gene description: phosphatidylinositol/phosphatidylcholine transfer protein SEC14
Primary source: FungiDB:YMR079W
Locus tag: YMR079W
See related: SGD:S000004684; AllianceGenome:SGD:S000004684; VEuPathDB:YMR079W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: PIT1
Summary: Enables phosphatidylcholine transporter activity and phosphatidylinositol transfer activity. Involved in several processes, including Golgi vesicle transport; ascospore formation; and negative regulation of phospholipid biosynthetic process. Located in Golgi membrane and cytosol. Used to study breast cancer. Orthologous to several human genes including SEC14L2 (SEC14 like lipid binding 2) and SEC14L3 (SEC14 like lipid binding 3). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See SEC14 in Genome Data Viewer

Location:: chromosome: XIII

Exon count:: 2

Sequence:: Chromosome: XIII; NC_001145.3 (424989..426059)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Biophysical parameters of the Sec14 phospholipid exchange cycle - Effect of lipid packing in membranes.
Title: Biophysical parameters of the Sec14 phospholipid exchange cycle - Effect of lipid packing in membranes.
Noncanonical regulation of phosphatidylserine metabolism by a Sec14-like protein and a lipid kinase.
Title: Noncanonical regulation of phosphatidylserine metabolism by a Sec14-like protein and a lipid kinase.
yeast Sec14-like PITP lipid-binding pockets indeed define diverse chemical microenvironments that translate into differential ligand-binding specificities across this protein family
Title: Functional diversification of the chemical landscapes of yeast Sec14-like phosphatidylinositol transfer protein lipid-binding cavities.
Data suggest that SEC14L and SFH1 undergo conformational changes as phosphatidylinositol/phosphatidylcholine bind to hydrophobic pockets/ligand binding sites. [review-like article]
Title: Sec14-like phosphatidylinositol-transfer proteins and diversification of phosphoinositide signalling outcomes.
Sec14 and Sec14 like proteins form central conduits to integrate multiple aspects of lipid metabolism with productive phosphoinositide signaling. (Review)
Title: Sec14 like PITPs couple lipid metabolism with phosphoinositide synthesis to regulate Golgi functionality.
The crystal structure of Sec14 family homologue (Sfh)3 is the first report of a dimeric Sec14 protein and provides clues for its functional study.
Title: Dimeric Sfh3 has structural changes in its binding pocket that are associated with a dimer-monomer state transformation induced by substrate binding.
Localization of lipid raft proteins to the plasma membrane is a major function of the phospholipid transfer protein Sec14.
Title: Localization of lipid raft proteins to the plasma membrane is a major function of the phospholipid transfer protein Sec14.
To launch a detailed structural and functional characterization of Sfh3, the recombinant protein was purified to homogeneity, diffraction-quality crystals were produced and a native X-ray data set was collected to 2.2 A resolution
Title: Crystallization and preliminary X-ray diffraction analysis of Sfh3, a member of the Sec14 protein superfamily.
Sec14 function was required for the route followed by Bgl2, whereas trafficking of other secreted proteins, including Hsp150, Cts1, Scw4, Scw10, Exg1, Cis3, and Ygp1, still occurred, indicating Sec14 regulates specific trans-Golgi export pathways.
Title: Phospholipid transfer protein Sec14 is required for trafficking from endosomes and regulates distinct trans-Golgi export pathways.
a regulatory role for Sec14 is a primary counter to action of the Kes1 sterol-binding protein that antagonizes PtdIns 4-OH kinase activity in vivo
Title: Functional anatomy of phospholipid binding and regulation of phosphoinositide homeostasis by proteins of the sec14 superfamily.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables phosphatidylcholine transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylcholine transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables phosphatidylinositol transfer activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol transfer activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in Golgi to plasma membrane protein transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in Golgi to vacuole transport	IGI Inferred from Genetic Interaction more info	PubMed
involved_in Golgi to vacuole transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in Golgi vesicle budding	IDA Inferred from Direct Assay more info	PubMed
involved_in ascospore formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intermembrane lipid transfer	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of phosphatidylcholine biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of phosphatidylcholine biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of phosphatidylglycerol biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phosphatidylinositol metabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in phosphatidylinositol metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phospholipid transport	IDA Inferred from Direct Assay more info	PubMed
involved_in phospholipid transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	IDA Inferred from Direct Assay more info	PubMed
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
located_in Golgi membrane	IDA Inferred from Direct Assay more info	PubMed
located_in Golgi membrane	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	HDA more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: phosphatidylinositol/phosphatidylcholine transfer protein SEC14

NP_013796.1

Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs; SEC14 has a paralog, YKL091C, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001145.3 Reference assembly

Range

424989..426059

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182578.1 → NP_013796.1 TPA: phosphatidylinositol/phosphatidylcholine transfer protein SEC14 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013796.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VZQ3, P24280

UniProtKB/TrEMBL

A6ZMD8, B3LLX2, C7GL45, C8ZET2, G2WKC7, N1NXA1

Conserved Domains (2) summary

smart00516
Location:99 → 269

SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)

smart01100
Location:34 → 79

CRAL_TRIO_N; CRAL/TRIO, N-terminal domain