SPA2 Spa2p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: SPA2
Gene description: Spa2p
Primary source: FungiDB:YLL021W
Locus tag: YLL021W
See related: SGD:S000003944; AllianceGenome:SGD:S000003944; VEuPathDB:YLL021W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: FUS6; PEA1; PPF1
Summary: Enables MAP-kinase scaffold activity. Involved in several processes, including bipolar cellular bud site selection; budding cell apical bud growth; and regulation of mating projection assembly. Located in several cellular components, including cellular bud neck; cellular bud tip; and mating projection tip. Part of incipient cellular bud site and polarisome. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See SPA2 in Genome Data Viewer

Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (100947..105347)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Spa2 remodels ADP-actin via molecular condensation under glucose starvation.
Title: Spa2 remodels ADP-actin via molecular condensation under glucose starvation.
A positive feedback loop involving the Spa2 SHD domain contributes to focal polarization.
Title: A positive feedback loop involving the Spa2 SHD domain contributes to focal polarization.
The molecular dynamics and reversibility of Aip5 condensates are regulated by scaffolding protein Spa2 via liquid-liquid phase separation both in vitro and in vivo. In the absence of Spa2, Aip5 condensates hamper cell growth and actin cable structures under stress treatment.
Title: Polarisome scaffolder Spa2-mediated macromolecular condensation of Aip5 for actin polymerization.
data indicate that Spa2 is recruited to the cleavage site to co-operate with the secretory vesicle system and particular actomyosin ring components to promote the incorporation of Chs2 into the so-called 'ingression progression complexes' during cytokinesis in budding yeast
Title: Cell polarity protein Spa2 coordinates Chs2 incorporation at the division site in budding yeast.
the pea2-M409L mutation results in decreased invasive filamentation and elongation, diminished activity of a Kss1p MAPK pathway reporter, decreased unipolar budding, and diminished binding of the polarisome protein Spa2p.
Title: Pooled segregant sequencing reveals genetic determinants of yeast pseudohyphal growth.
Therefore, we suggest that Mid1 function is influenced by Spa2 function through polarized morphogenesis.
Title: Polarized morphogenesis regulator Spa2 is required for the function of putative stretch-activated Ca2+-permeable channel component Mid1 in Saccharomyces cerevisiae.
findings show Cdc42 serves an essential function in the establishment of bud site by catalyzing translocation of bud-site components such as Spa2 to the cell cortex; role of Cdc42 in the cortical localization of Spa2 appears to be independent of Cdc24
Title: Cdc42-dependent localization of polarisome component Spa2 to the incipient bud site is independent of the GDP/GTP exchange factor Cdc24.
Spa2p is involved in cytokinesis and cell wall morphogenesis.
Title: Cell polarity protein Spa2P associates with proteins involved in actin function in Saccharomyces cerevisiae.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables MAP-kinase scaffold activity	IBA Inferred from Biological aspect of Ancestor more info
enables MAP-kinase scaffold activity	IDA Inferred from Direct Assay more info	PubMed
enables MAP-kinase scaffold activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in MAPK cascade	IEA Inferred from Electronic Annotation more info
involved_in bipolar cellular bud site selection	IBA Inferred from Biological aspect of Ancestor more info
involved_in bipolar cellular bud site selection	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in budding cell apical bud growth	IGI Inferred from Genetic Interaction more info	PubMed
involved_in budding cell apical bud growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular hyperosmotic response	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular hyperosmotic response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to acidic pH	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of cell polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in invasive filamentous growth	IBA Inferred from Biological aspect of Ancestor more info
involved_in invasive filamentous growth	IGI Inferred from Genetic Interaction more info	PubMed
involved_in pseudohyphal growth	IBA Inferred from Biological aspect of Ancestor more info
involved_in pseudohyphal growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of actin cytoskeleton organization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell shape	IEA Inferred from Electronic Annotation more info
involved_in regulation of protein localization	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in actomyosin contractile ring	IBA Inferred from Biological aspect of Ancestor more info
located_in cell cortex	IDA Inferred from Direct Assay more info	PubMed
is_active_in cell cortex of growing cell tip	IBA Inferred from Biological aspect of Ancestor more info
located_in cell tip	IEA Inferred from Electronic Annotation more info
is_active_in cellular bud neck	IBA Inferred from Biological aspect of Ancestor more info
located_in cellular bud neck	IDA Inferred from Direct Assay more info	PubMed
is_active_in cellular bud tip	IBA Inferred from Biological aspect of Ancestor more info
located_in cellular bud tip	IDA Inferred from Direct Assay more info	PubMed
is_active_in cellular bud tip	IMP Inferred from Mutant Phenotype more info	PubMed
located_in incipient cellular bud site	IBA Inferred from Biological aspect of Ancestor more info
located_in incipient cellular bud site	IDA Inferred from Direct Assay more info	PubMed
is_active_in mating projection tip	IBA Inferred from Biological aspect of Ancestor more info
located_in mating projection tip	IDA Inferred from Direct Assay more info	PubMed
part_of polarisome	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Spa2p

NP_013079.1

Component of the polarisome; functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate; coding sequence contains length polymorphisms in different strains; SPA2 has a paralog, SPH1, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001144.5 Reference assembly

Range

100947..105347

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181841.1 → NP_013079.1 TPA: Spa2p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013079.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VXY3, P23201

UniProtKB/TrEMBL

A0A8H4BXU1, N1P021

Conserved Domains (2) summary

pfam08518
Location:94 → 122

GIT_SHD; Spa2 homology domain (SHD) of GIT

pfam15140
Location:876 → 1042

DUF4573; Domain of unknown function (DUF4573)