MEP2 ammonium permease MEP2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: MEP2
Official Full Name: ammonium permease MEP2
Primary source: SGD:S000005086
Locus tag: YNL142W
See related: AllianceGenome:SGD:S000005086; FungiDB:YNL142W; VEuPathDB:YNL142W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables ammonium channel activity. Involved in several processes, including ammonium transmembrane transport; nitrogen utilization; and pseudohyphal growth. Located in plasma membrane. Used to study renal tubular acidosis. [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See MEP2 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (357453..358952)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Coexistence of Ammonium Transporter and Channel Mechanisms in Amt-Mep-Rh Twin-His Variants Impairs the Filamentation Signaling Capacity of Fungal Mep2 Transceptors.
Title: Coexistence of Ammonium Transporter and Channel Mechanisms in Amt-Mep-Rh Twin-His Variants Impairs the Filamentation Signaling Capacity of Fungal Mep2 Transceptors.
Study shows that S. cerevisiae filamentation is intimately related to Mep2 transport activity. Mep2 variants lacking the C-terminal domain but containing specific pore mutations enabling transport can still allow filamentation induction. Mep2H194E pore variant shows increased competence to acidify the submembrane pH while losing the signaling capability.
Title: Yeast filamentation signaling is connected to a specific substrate translocation mechanism of the Mep2 transceptor.
X-ray crystal structures are being reported of the Mep2 orthologues from Saccharomyces cerevisiae and Candida albicans showing that under nitrogen-sufficient conditions the transporters are not phosphorylated and present in closed, inactive conformations.
Title: Structural basis for Mep2 ammonium transceptor activation by phosphorylation.
Mep2-like proteins are nutrient sensing transceptors that govern cellular differentiation
Title: A Mep2-dependent transcriptional profile links permease function to gene expression during pseudohyphal growth in Saccharomyces cerevisiae.
an ammonium permease MEP2-based sensing mechanism for rapid activation of the protein kinase A pathway
Title: Ammonium permease-based sensing mechanism for rapid ammonium activation of the protein kinase A pathway in yeast.
The growth impairment of cells lacking Npr1 on many nitrogen sources is shared by cells deprived of the three Mep proteins and is a consequence of deficient ammonium retrieval.
Title: The yeast ammonium transport protein Mep2 and its positive regulator, the Npr1 kinase, play an important role in normal and pseudohyphal growth on various nitrogen media through retrieval of excreted ammonium.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ammonium channel activity	IBA Inferred from Biological aspect of Ancestor more info
enables ammonium channel activity	IDA Inferred from Direct Assay more info	PubMed
enables ammonium channel activity	IEA Inferred from Electronic Annotation more info
enables ammonium channel activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in ammonium transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in ammonium transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in ammonium transmembrane transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nitrogen utilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of filamentous growth of a population of unicellular organisms	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pseudohyphal growth	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: ammonium permease MEP2

NP_014257.1

Ammonium permease involved in regulation of pseudohyphal growth; belongs to Mep-Amt-Rh family of well-conserved ammonium (NH4+) transporters that includes human Rh factors; expression is under nitrogen catabolite repression regulation; activity is controlled by phospho-silencing; phosphorylation of Mep2 mediated by Npr1; dephosphorylation involves Psr1p and Psr2p; Twin-His motif ensures transport specificity

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

357453..358952

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182980.1 → NP_014257.1 TPA: ammonium permease MEP2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014257.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W140, P41948

UniProtKB/TrEMBL

A6ZRU1, B3LNZ8, B5VQQ7, C7GKA6, N1P4A3

Conserved Domains (1) summary

COG0004
Location:27 → 447

AmtB; Ammonia channel protein AmtB [Inorganic ion transport and metabolism]