RPB8 DNA-directed RNA polymerase core subunit RPB8 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RPB8
Official Full Name: DNA-directed RNA polymerase core subunit RPB8
Primary source: SGD:S000005750
Locus tag: YOR224C
See related: AllianceGenome:SGD:S000005750; FungiDB:YOR224C; VEuPathDB:YOR224C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Contributes to 5'-3' RNA polymerase activity. Involved in tRNA transcription by RNA polymerase III; transcription by RNA polymerase I; and transcription by RNA polymerase II. Part of RNA polymerase I complex; RNA polymerase II, core complex; and RNA polymerase III complex. Orthologous to human POLR2H (RNA polymerase II, I and III subunit H). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See RPB8 in Genome Data Viewer

Location:: chromosome: XV

Exon count:: 1

Sequence:: Chromosome: XV; NC_001147.6 (760825..761265, complement)

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data show that Top2 is required redundantly with Top1 for optimal recruitment of RNA polymerase II at their promoters.
Title: Topoisomerase II binds nucleosome-free DNA and acts redundantly with topoisomerase I to enhance recruitment of RNA Pol II in budding yeast.

Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-directed 5'-3' RNA polymerase activity	IEA Inferred from Electronic Annotation more info
contributes_to RNA polymerase I activity	IBA Inferred from Biological aspect of Ancestor more info
contributes_to RNA polymerase I activity	IDA Inferred from Direct Assay more info	PubMed
contributes_to RNA polymerase II activity	IBA Inferred from Biological aspect of Ancestor more info
contributes_to RNA polymerase II activity	IDA Inferred from Direct Assay more info	PubMed
contributes_to RNA polymerase III activity	IBA Inferred from Biological aspect of Ancestor more info
contributes_to RNA polymerase III activity	IDA Inferred from Direct Assay more info	PubMed
contributes_to RNA-dependent RNA polymerase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in RNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in ribosome biogenesis	IEA Inferred from Electronic Annotation more info
involved_in tRNA transcription by RNA polymerase III	IDA Inferred from Direct Assay more info	PubMed
involved_in transcription by RNA polymerase I	IDA Inferred from Direct Assay more info	PubMed
involved_in transcription by RNA polymerase I	IPI Inferred from Physical Interaction more info	PubMed
involved_in transcription by RNA polymerase II	IPI Inferred from Physical Interaction more info	PubMed
involved_in transcription by RNA polymerase III	IGI Inferred from Genetic Interaction more info	PubMed
involved_in transcription by RNA polymerase III	IPI Inferred from Physical Interaction more info	PubMed

Component	Evidence Code	Pubs
part_of DNA-directed RNA polymerase complex	IEA Inferred from Electronic Annotation more info
part_of RNA polymerase I complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RNA polymerase I complex	IDA Inferred from Direct Assay more info	PubMed
part_of RNA polymerase II, core complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RNA polymerase II, core complex	IDA Inferred from Direct Assay more info	PubMed
part_of RNA polymerase III complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RNA polymerase III complex	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: DNA-directed RNA polymerase core subunit RPB8

NP_014867.1

RNA polymerase subunit ABC14.5; common to RNA polymerases I, II, and III

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6 Reference assembly

Range

760825..761265 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183643.1 → NP_014867.1 TPA: DNA-directed RNA polymerase core subunit RPB8 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014867.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W2S9, P20436

UniProtKB/TrEMBL

A6ZP66, B3LJP7, C7GWF9, C8ZGX8, G2WN95, N1NVW0

Conserved Domains (1) summary

pfam03870
Location:10 → 145

RNA_pol_Rpb8; RNA polymerase Rpb8