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    GCS1 GTPase-activating protein GCS1 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 851372, updated on 2-Nov-2024

    Summary

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    Official Symbol
    GCS1
    Official Full Name
    GTPase-activating protein GCS1
    Primary source
    SGD:S000002385
    Locus tag
    YDL226C
    See related
    AllianceGenome:SGD:S000002385; FungiDB:YDL226C; VEuPathDB:YDL226C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables GTPase activator activity and actin binding activity. Involved in Golgi vesicle transport; actin filament organization; and mitotic cell cycle. Located in cytoskeleton; endosome; and trans-Golgi network. Orthologous to human ARFGAP1 (ADP ribosylation factor GTPase activating protein 1). [provided by Alliance of Genome Resources, Nov 2024]
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    Genomic context

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    See GCS1 in Genome Data Viewer
    Location:
    chromosome: IV
    Exon count:
    1
    Sequence:
    Chromosome: IV; NC_001136.10 (51115..52173, complement)

    Chromosome IV - NC_001136.10Genomic Context describing neighboring genes Neighboring gene Hsp70 family ATPase SSB1 Neighboring gene Hop Neighboring gene septin SHS1 Neighboring gene Whi4p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001136.10

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. A member of a novel family of yeast 'zn-finger' proteins mediates the transition from stationary phase to cell proliferation. Ireland LS, et al. EMBO J, 1994 Aug 15. PMID 8070409, Free PMC Article
    2. Candida albicans AGE3, the ortholog of the S. cerevisiae ARF-GAP-encoding gene GCS1, is required for hyphal growth and drug resistance. Lettner T, et al. PLoS One, 2010 Aug 5. PMID 20700541, Free PMC Article
    3. Expression, analysis, and properties of yeast ADP-ribosylation factor (ARF) GTPase activating proteins (GAPs) Gcs1 and Glo3. Poon PP, et al. Methods Enzymol, 2001. PMID 11210551
    4. Mitochondrial DNA loss by yeast reentry-mutant cells conditionally unable to proliferate from stationary phase. Filipak M, et al. Curr Genet, 1992 Dec. PMID 1473178
    5. A yeast mutant conditionally defective only for reentry into the mitotic cell cycle from stationary phase. Drebot MA, et al. Proc Natl Acad Sci U S A, 1987 Nov. PMID 3317397, Free PMC Article

    See all (102) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. ALPS and the predicted AH region are involved in the regulation and function of Gcs1p by interacting with membrane phospholipids and vesicle proteins.
      Title: Identification of a second amphipathic lipid-packing sensor-like motif that contributes to Gcs1p function in the early endosome-to-TGN pathway.
    2. The ArfGAP Gcs1p accelerates vesicle (v)-target membrane (t)-SNARE complex formation in vitro, indicating that ArfGAPs may act as folding chaperones.
      Title: Interaction of SNAREs with ArfGAPs precedes recruitment of Sec18p/NSF.
    3. Taken together, these results indicate that GCS1 is essential for sporulation and suggest that GCS1 positively regulates SPO14.
      Title: The Arf-GTPase-activating protein Gcs1p is essential for sporulation and regulates the phospholipase D Spo14p.
    4. These results suggest that Cdc50p-Drs2p plays an important role in the Arf1p/Gcs1-mediated formation of clathrin-coated vesicles for the retrieval pathway from early endosomes to the trans-Golgi network.
      Title: The functional relationship between the Cdc50p-Drs2p putative aminophospholipid translocase and the Arf GAP Gcs1p in vesicle formation in the retrieval pathway from yeast early endosomes to the TGN.
    5. Release of Arl1p from membranes in cells requires the hydrolysis of bound GTP, which is accelerated by Gcs1p.
      Title: Role for Gcs1p in regulation of Arl1p at trans-Golgi compartments.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables GTPase activator activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables GTPase activator activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables actin binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables zinc ion binding RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in COPI coating of Golgi vesicle IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in Golgi to plasma membrane protein transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in actin filament organization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in endoplasmic reticulum to Golgi vesicle-mediated transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mitotic cell cycle IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mitotic cell cycle process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in Golgi apparatus IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in Golgi membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytoskeleton IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    located_in cytosol HDA PubMed 
    located_in endosome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in mitochondrion IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in perinuclear region of cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in trans-Golgi network IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    GTPase-activating protein GCS1
    NP_010055.1
    • ADP-ribosylation factor GTPase activating protein (ARF GAP); involved in ER-Golgi transport; required for prospore membrane formation; regulates phospholipase Spo14p; shares functional similarity with Glo3p; GCS1 has a paralog, SPS18, that arose from the whole genome duplication

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001136.10 Reference assembly

      Range
      51115..52173 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001180286.1 → NP_010055.1  TPA: GTPase-activating protein GCS1 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_010055.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VRC9, P35197
      UniProtKB/TrEMBL
      A6ZXA8, C7GSV2, C8Z6P3, G2WBU2, N1P5U7
      Conserved Domains (1) summary
      COG5347
      Location:4 → 350
      COG5347; GTPase-activating protein that regulates ARFs (ADP-ribosylation factors), involved in ARF-mediated vesicular transport [Intracellular trafficking and secretion]
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P35197.1

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