aak-2 5'-AMP-activated protein kinase catalytic subunit alpha-2 [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: aak-2
Official Full Name: 5'-AMP-activated protein kinase catalytic subunit alpha-2
Primary source: WormBase:WBGene00020142
Locus tag: CELE_T01C8.1
See related: AllianceGenome:WB:WBGene00020142
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables AMP-activated protein kinase activity. Involved in several processes, including determination of adult lifespan; nematode pharyngeal pumping; and regulation of protein secretion. Located in axon; cytoplasm; and neuronal cell body. Is expressed in several structures, including body wall musculature; excretory cell; nervous system; pharynx; and reproductive system. Human ortholog(s) of this gene implicated in Huntington's disease; breast cancer; colon cancer; and type 2 diabetes mellitus. Orthologous to human PRKAA1 (protein kinase AMP-activated catalytic subunit alpha 1) and PRKAA2 (protein kinase AMP-activated catalytic subunit alpha 2). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See aak-2 in Genome Data Viewer

Location:: chromosome: X

Exon count:: 10

Sequence:: Chromosome: X; NC_003284.9 (16796087..16803398)

Chromosome X - NC_003284.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003284.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

ROS-mediated relationships between metabolism and DAF-16 subcellular localization in Caenorhabditis elegans revealed by a novel fluorometric method.
Title: ROS-mediated relationships between metabolism and DAF-16 subcellular localization in Caenorhabditis elegans revealed by a novel fluorometric method.
AMPK functions downstream of, or in parallel with DAF-18/PTEN and insulin/IGF-1 signaling to inhibit PP2A function.
Title: C. elegans PTEN and AMPK block neuroblast divisions by inhibiting a BMP-insulin-PP2A-MAPK pathway.
mir-259 functioned in pharynx-intestinal valve and RSKS-1 functioned in pharynx to regulate MWCNTs toxicity. Furthermore, RSKS-1 regulated MWCNTs toxicity by suppressing the function of AAK-2-DAF-16 signaling cascade. Our results will strengthen our understanding the microRNAs mediated protection mechanisms for animals against the toxicity from certain nanomaterials.
Title: Function of RSKS-1-AAK-2-DAF-16 signaling cascade in enhancing toxicity of multi-walled carbon nanotubes can be suppressed by mir-259 activation in Caenorhabditis elegans.
Transcription factors CEP-1 and CEH-23 collaborate with AAK-2 to modulate longevity in Caenorhabditis elegans.
Title: Transcription factors CEP-1/p53 and CEH-23 collaborate with AAK-2/AMPK to modulate longevity in Caenorhabditis elegans.
he results revealed that RNAi-mediated inactivation of several genes restores gonad elongation, including aak-2, which encodes the catalytic subunit of AMP-activated protein kinase (AMPK). We then generated triple mutant worms for cnnm-1; cnnm-3; aak-2 and confirmed that the aak-2 mutation also suppressed the defective gonadal elongation in cnnm-1; cnnm-3 mutant worms.
Title: Mg2+ Extrusion from Intestinal Epithelia by CNNM Proteins Is Essential for Gonadogenesis via AMPK-TORC1 Signaling in Caenorhabditis elegans.
AAK-2 regulates oxidative metabolism in Caenorhabditis elegans through the NHR-49 and MDT-15 transcriptional regulators.
Title: AMP-Activated Protein Kinase Regulates Oxidative Metabolism in Caenorhabditis elegans through the NHR-49 and MDT-15 Transcriptional Regulators.
Knockdown of Indy/CeNAC2 increased life span by 22+/-3 % compared to controls, together with a decrease in whole body fat content by ~50%. Indy/CeNAC2 reduction also increased the activation of the intracellular energy sensor AMPK/aak2.
Title: Knockdown of Indy/CeNac2 extends Caenorhabditis elegans life span by inducing AMPK/aak-2.
As in elevated serotonin signaling, inactivation of AAK-2 in the ASI neurons caused enhanced secretion of dense core vesicles from these neurons.
Title: Loss of a neural AMP-activated kinase mimics the effects of elevated serotonin on fat, movement, and hormonal secretions.
AMPK and HIF-1 may control immunity and longevity tightly by acting as feedback regulators of ROS
Title: Feedback regulation via AMPK and HIF-1 mediates ROS-dependent longevity in Caenorhabditis elegans.
An essential output of RSKS-1 in axon regrowth is the metabolic sensor AMP kinase, AAK-2.
Title: S6 kinase inhibits intrinsic axon regeneration capacity via AMP kinase in Caenorhabditis elegans.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

T01C8.1 (e-PCR)
- UniSTS:314836

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables AMP-activated protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables AMP-activated protein kinase activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cellular response to glucose starvation	IBA Inferred from Biological aspect of Ancestor more info
involved_in determination of adult lifespan	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of TOR signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of protein secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nematode pharyngeal pumping	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of dauer larval development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of protein secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in serotonin receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in axon	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
part_of nucleotide-activated protein kinase complex	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: 5'-AMP-activated protein kinase catalytic subunit alpha-2

NP_001024868.1

Confirmed by transcript evidence

NP_510710.2

Confirmed by transcript evidence

NP_510711.2

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003284.9 Reference assembly

Range

16796087..16803398

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_078310.8 → NP_510711.2 5'-AMP-activated protein kinase catalytic subunit alpha-2 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_510711.2

Status: REVIEWED

UniProtKB/Swiss-Prot

Q95ZQ4

Conserved Domains (4) summary

cd12122
Location:460 → 622

AMPKA_C; C-terminal regulatory domain of 5'-AMP-activated protein kinase (AMPK) alpha catalytic subunit

smart00220
Location:87 → 339

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd14336
Location:356 → 417

UBA_AID_AMPKalpha; UBA-like autoinhibitory domain (AID) found in vertebrate 5'-AMP-activated protein kinase catalytic alpha (AMPKalpha) subunits

cd14079
Location:84 → 339

STKc_AMPK_alpha; Catalytic domain of the Alpha subunit of the Serine/Threonine Kinase, AMP-activated protein kinase
NM_078309.7 → NP_510710.2 5'-AMP-activated protein kinase catalytic subunit alpha-2 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_510710.2

Status: REVIEWED

UniProtKB/Swiss-Prot

Q22068, Q86FL6, Q95ZQ4

Conserved Domains (4) summary

cd12122
Location:462 → 624

AMPKA_C; C-terminal regulatory domain of 5'-AMP-activated protein kinase (AMPK) alpha catalytic subunit

smart00220
Location:87 → 339

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd14336
Location:356 → 419

UBA_AID_AMPKalpha; UBA-like autoinhibitory domain (AID) found in vertebrate 5'-AMP-activated protein kinase catalytic alpha (AMPKalpha) subunits

cd14079
Location:84 → 339

STKc_AMPK_alpha; Catalytic domain of the Alpha subunit of the Serine/Threonine Kinase, AMP-activated protein kinase
NM_001029697.6 → NP_001024868.1 5'-AMP-activated protein kinase catalytic subunit alpha-2 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001024868.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q95ZQ4

Conserved Domains (4) summary

cd12122
Location:398 → 560

AMPKA_C; C-terminal regulatory domain of 5'-AMP-activated protein kinase (AMPK) alpha catalytic subunit

smart00220
Location:25 → 277

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd14336
Location:294 → 355

UBA_AID_AMPKalpha; UBA-like autoinhibitory domain (AID) found in vertebrate 5'-AMP-activated protein kinase catalytic alpha (AMPKalpha) subunits

cd14079
Location:22 → 277

STKc_AMPK_alpha; Catalytic domain of the Alpha subunit of the Serine/Threonine Kinase, AMP-activated protein kinase