CHS2 chitin synthase CHS2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: CHS2
Official Full Name: chitin synthase CHS2
Primary source: SGD:S000000242
Locus tag: YBR038W
See related: AllianceGenome:SGD:S000000242; FungiDB:YBR038W; VEuPathDB:YBR038W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables chitin synthase activity. Involved in chitin biosynthetic process and mitotic actomyosin contractile ring maintenance. Located in cellular bud neck. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See CHS2 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 1

Sequence:: Chromosome: II; NC_001134.8 (311898..314789)

Chromosome II - NC_001134.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

data indicate that Spa2 is recruited to the cleavage site to co-operate with the secretory vesicle system and particular actomyosin ring components to promote the incorporation of Chs2 into the so-called 'ingression progression complexes' during cytokinesis in budding yeast
Title: Cell polarity protein Spa2 coordinates Chs2 incorporation at the division site in budding yeast.
the mitotic spindle breakage observed in endocytosis mutants can be rescued either by deleting or inhibiting the activities of, CHS2, CHS3 and FKS1, which are involved in septum formation. The findings from our study highlight the importance of timely endocytosis of cytokinetic enzymes at the division site in safeguarding mitotic spindle integrity during mitotic exit.
Title: Timely Endocytosis of Cytokinetic Enzymes Prevents Premature Spindle Breakage during Mitotic Exit.
the cytoplasmic N-terminal domain of Chs2p, which contains the CDK1 phosphorylation sites, interacts with the COPII component Sec24p in a yeast two-hybrid assay and that phosphomimetic substitutions of serines at the CDK1 consensus sites reduces the interaction.
Title: Phosphorylation of Chs2p regulates interaction with COPII.
Dbf2, a conserved mitotic exit kinase, localizes to the division site after Chs2 and directly phosphorylates Chs2 on several residues
Title: Mitotic exit kinase Dbf2 directly phosphorylates chitin synthase Chs2 to regulate cytokinesis in budding yeast.
Chs2 endoplasmic reticulum export requires the direct reversal of the inhibitory Cdk1 phosphorylation sites by Cdc14 phosphatase
Title: Dependence of Chs2 ER export on dephosphorylation by cytoplasmic Cdc14 ensures that septum formation follows mitosis.
phosphorylation of the N-terminal domain is important for Chs2 stability, these sites might play an important role in the cell cycle-dependent degradation of the enzyme, and thus in cell division
Title: Yeast chitin synthase 2 activity is modulated by proteolysis and phosphorylation.
Chs2, as the chitin synthase that produces the primary septum, couples the assembly of the extracellular matrix with the dynamics of the contractile ring during cytokinesis.
Title: Cell cycle-regulated trafficking of Chs2 controls actomyosin ring stability during cytokinesis.
The dependence of Chs2p exit from the ER followed by its transport to the neck upon mitotic exit ensures that septum formation occurs only after the completion of mitotic events.
Title: Exit from mitosis triggers Chs2p transport from the endoplasmic reticulum to mother-daughter neck via the secretory pathway in budding yeast.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables chitin synthase activity	IBA Inferred from Biological aspect of Ancestor more info
enables chitin synthase activity	IDA Inferred from Direct Assay more info	PubMed
enables chitin synthase activity	IEA Inferred from Electronic Annotation more info
enables glycosyltransferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cell wall organization	IEA Inferred from Electronic Annotation more info
involved_in chitin biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in chitin biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in chitin biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic actomyosin contractile ring contraction	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cell periphery	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cell septum	IBA Inferred from Biological aspect of Ancestor more info
located_in cellular bud neck	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: chitin synthase CHS2

NP_009594.1

EC 2.4.1.16

Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001134.8 Reference assembly

Range

311898..314789

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178386.1 → NP_009594.1 TPA: chitin synthase CHS2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009594.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VQ38, P14180

UniProtKB/TrEMBL

A6ZKY2, B3LNA7, B5VE04, C7GXX4, D3UED4, G2W943, N1P6F8

Conserved Domains (2) summary

cd04190
Location:294 → 612

Chitin_synth_C; C-terminal domain of Chitin Synthase catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin

pfam08407
Location:237 → 297

Chitin_synth_1N; Chitin synthase N-terminal