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    CRN1 Crn1p [ Saccharomyces cerevisiae S288C ]

    Gene ID: 851148, updated on 2-Nov-2024

    Summary

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    Official Symbol
    CRN1
    Official Full Name
    Crn1p
    Primary source
    SGD:S000004421
    Locus tag
    YLR429W
    See related
    AllianceGenome:SGD:S000004421; FungiDB:YLR429W; VEuPathDB:YLR429W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables Arp2/3 complex binding activity; cytoskeletal protein binding activity; and protein-macromolecule adaptor activity. Involved in several processes, including actin cortical patch localization; actin filament organization; and regulation of Arp2/3 complex-mediated actin nucleation. Located in actin cortical patch. Human ortholog(s) of this gene implicated in coronin-1A deficiency. Orthologous to several human genes including CORO6 (coronin 6). [provided by Alliance of Genome Resources, Nov 2024]
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    Genomic context

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    See CRN1 in Genome Data Viewer
    Location:
    chromosome: XII
    Exon count:
    1
    Sequence:
    Chromosome: XII; NC_001144.5 (990777..992732)

    Chromosome XII - NC_001144.5Genomic Context describing neighboring genes Neighboring gene Tda5p Neighboring gene Mag2p Neighboring gene DNA/RNA helicase SEN1 Neighboring gene Atg23p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001144.5

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The role of Saccharomyces cerevisiae coronin in the actin and microtubule cytoskeletons. Heil-Chapdelaine RA, et al. Curr Biol, 1998 Nov 19. PMID 9822583
    2. The intrinsically disordered region of coronins fine-tunes oligomerization and actin polymerization. Han X, et al. Cell Rep, 2023 Jun 27. PMID 37269287
    3. Cryo-EM reveals different coronin binding modes for ADP- and ADP-BeFx actin filaments. Ge P, et al. Nat Struct Mol Biol, 2014 Dec. PMID 25362487, Free PMC Article
    4. Overlapping and distinct functions for cofilin, coronin and Aip1 in actin dynamics in vivo. Lin MC, et al. J Cell Sci, 2010 Apr 15. PMID 20332110, Free PMC Article
    5. Coronin promotes the rapid assembly and cross-linking of actin filaments and may link the actin and microtubule cytoskeletons in yeast. Goode BL, et al. J Cell Biol, 1999 Jan 11. PMID 9885246, Free PMC Article

    See all (75) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Using multi-color total internal reflection fluorescence microscopy, the study shows that Crn1 enhances Cof1-mediated severing by accelerating Cof1 binding to actin filament sides.
      Title: Coronin Enhances Actin Filament Severing by Recruiting Cofilin to Filament Sides and Altering F-Actin Conformation.
    2. The study reports cryo-EM structures for ADP-bound and ADP-beryllium fluoride (ADP-BeFx, an ADP-Pi mimic)-bound actin filaments in complex with the beta-propeller domain of yeast coronin 1 (crn1), at 8.6-A resolution.
      Title: Cryo-EM reveals different coronin binding modes for ADP- and ADP-BeFx actin filaments.
    3. development of a direct tethering recruitment model for activation of Arp2/3 complex by Crn1
      Title: Mechanism of a concentration-dependent switch between activation and inhibition of Arp2/3 complex by coronin.
    4. Conservation of the actin-binding surfaces across distant species and in all three major classes of coronin isoforms suggests that the nature of the coronin-actin association may be similar in other family members.
      Title: Functional surfaces on the actin-binding protein coronin revealed by systematic mutagenesis.
    5. Results suggest that that coronin polarizes the spatial distribution and activity of cofilin to promote selective disassembly of older actin filaments.
      Title: Coronin switches roles in actin disassembly depending on the nucleotide state of actin.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables Arp2/3 complex binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables actin binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables actin filament binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables actin filament binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables microtubule binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein-macromolecule adaptor activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in actin cortical patch localization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in actin filament organization IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in actin filament organization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in actin filament organization IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    involved_in microtubule-based process IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    involved_in negative regulation of Arp2/3 complex-mediated actin nucleation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in negative regulation of Arp2/3 complex-mediated actin nucleation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of Arp2/3 complex-mediated actin nucleation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in positive regulation of Arp2/3 complex-mediated actin nucleation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in actin cortical patch IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    Crn1p
    NP_013533.1
    • Coronin; cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity; plays a role in regulation of actin patch assembly

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001144.5 Reference assembly

      Range
      990777..992732
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001182317.1 → NP_013533.1  TPA: Crn1p [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_013533.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VZ64, Q06440
      UniProtKB/TrEMBL
      B3RHT8, N1NYV0
      Conserved Domains (4) summary
      sd00039
      Location:85 → 138
      7WD40; WD40 repeat [structural motif]
      NF033645
      Location:428 → 600
      pilus_FilE; putative pilus assembly protein FilE
      NF038014
      Location:521 → 641
      Chlamy_inclu_1; inclusion-associated protein
      cl29593
      Location:9 → 424
      WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q06440.1

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