RIF2 Rif2p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RIF2
Official Full Name: Rif2p
Primary source: SGD:S000004445
Locus tag: YLR453C
See related: AllianceGenome:SGD:S000004445; FungiDB:YLR453C; VEuPathDB:YLR453C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables telomeric DNA binding activity. Involved in telomere capping and telomere maintenance via telomerase. Located in chromosome, telomeric region. Part of shelterin complex. [provided by Alliance of Genome Resources, Oct 2024]
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Genomic context

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See RIF2 in Genome Data Viewer

Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (1041799..1042986, complement)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Rif2 interaction with Rad50 counteracts Tel1 functions in checkpoint signalling and DNA tethering by releasing Tel1 from MRX binding.
Title: Rif2 interaction with Rad50 counteracts Tel1 functions in checkpoint signalling and DNA tethering by releasing Tel1 from MRX binding.
Rif2 protects Rap1-depleted telomeres from MRX-mediated degradation in Saccharomyces cerevisiae.
Title: Rif2 protects Rap1-depleted telomeres from MRX-mediated degradation in Saccharomyces cerevisiae.
Mechanism of MRX inhibition by Rif2 at telomeres.
Title: Mechanism of MRX inhibition by Rif2 at telomeres.
Rif2 role in the telomere protection at uncapped telomeres.
Title: Tel1 and Rif2 oppositely regulate telomere protection at uncapped telomeres in Saccharomyces cerevisiae.
We identified an N-terminal domain in Rif2 that is essential for length regulation, which we have termed BAT domain for Blocks Addition of Telomeres
Title: Regulation of Telomere Length Requires a Conserved N-Terminal Domain of Rif2 in Saccharomyces cerevisiae.
The role of Tel1 in promoting MRX accumulation to double-strand breaks (DSBs)is counteracted by Rif2, which is recruited to DSBs. We also found that Rif2 enhances ATP hydrolysis by MRX and attenuates MRX function in end-tethering.
Title: Tel1 and Rif2 Regulate MRX Functions in End-Tethering and Repair of DNA Double-Strand Breaks.
The X-ray structures of Rif1 and Rif2 bound to the Rap1 C-terminal domain and that of the Rif1 C terminus are presented. Both Rif1 and Rif2 have separable and independent Rap1-binding epitopes, allowing Rap1 binding over large distances (42-110 A).
Title: Rif1 and Rif2 shape telomere function and architecture through multivalent Rap1 interactions.
The telomeric-bound protein, Rif2, was also found to promote a telomere fold-back through the recruitment of Rpd3L to telomeres
Title: Rif2 promotes a telomere fold-back structure through Rpd3L recruitment in budding yeast.
Rif2 is unable to negatively regulate processing and lengthening at TEL1-hy909 telomeres, indicating that the Tel1-hy909 variant overcomes the inhibitory activity exerted by Rif2 on Mre11-Rad50-Xrs2
Title: A balance between Tel1 and Rif2 activities regulates nucleolytic processing and elongation at telomeres.
Data show that DNA ends that are protected by Yku and Rif2 involves multiple functions of the MRX complex that do not necessarily require its nuclease activity.
Title: The MRX complex plays multiple functions in resection of Yku- and Rif2-protected DNA ends.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables telomeric DNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in telomere capping	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in telomere maintenance via telomerase	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in chromosome, telomeric region	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome, telomeric region	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of shelterin complex	IPI Inferred from Physical Interaction more info	PubMed

General protein information

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Preferred Names: Rif2p

NP_013558.3

Protein that binds to the Rap1p C-terminus; inhibits MRX-dependent Tel1p activity by acting directly on Rad50p and discharging its activated ATP-bound state, thereby rendering the MRX complex incompetent for Tel1p activation; acts synergistically with Rif1p to help control telomere length, establish telomeric silencing; deletion results in telomere elongation; RIF2 has paralog ORC4 that arose from the whole genome duplication