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    mad3 mitotic spindle checkpoint protein Mad3 [ Schizosaccharomyces pombe (fission yeast) ]

    Gene ID: 2539219, updated on 18-Sep-2024

    Summary

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    Official Symbol
    mad3
    Official Full Name
    mitotic spindle checkpoint protein Mad3
    Primary source
    PomBase:SPCC1795.01c
    Locus tag
    SPOM_SPCC1795.01C
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Schizosaccharomyces pombe (strain: 972h-)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces
    Also known as
    SPCC895.02
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    Genomic context

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    See mad3 in Genome Data Viewer
    Location:
    chromosome: III
    Exon count:
    1
    Sequence:
    Chromosome: III; NC_003421.2 (997416..998839)

    Chromosome III - NC_003421.2Genomic Context describing neighboring genes Neighboring gene Golgi UDP-galactose transmembrane transporter Gms1 Neighboring gene ncRNA Neighboring gene CaCA proton/calcium exchanger Neighboring gene ncRNA Neighboring gene ncRNA Neighboring gene ncRNA Neighboring gene SUA5/yciO/yrdC family protein Sua5 Neighboring gene SNARE protein Ufe1

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003421.2

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Structure of the mitotic checkpoint complex. Chao WC, et al. Nature, 2012 Mar 21. PMID 22437499
    2. The A78V mutation in the Mad3-like domain of Schizosaccharomyces pombe Bub1p perturbs nuclear accumulation and kinetochore targeting of Bub1p, Bub3p, and Mad3p and spindle assembly checkpoint function. Kadura S, et al. Mol Biol Cell, 2005 Jan. PMID 15525673, Free PMC Article
    3. S. pombe aurora kinase/survivin is required for chromosome condensation and the spindle checkpoint attachment response. Petersen J, et al. Curr Biol, 2003 Apr 1. PMID 12676091
    4. Deletion of Mia1/Alp7 activates Mad2-dependent spindle assembly checkpoint in fission yeast. Sato M, et al. Nat Cell Biol, 2003 Sep. PMID 12951601
    5. The Bub1-TPR Domain Interacts Directly with Mad3 to Generate Robust Spindle Checkpoint Arrest. Leontiou I, et al. Curr Biol, 2019 Jul 22. PMID 31257143, Free PMC Article

    See all (31) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. crystal structure of Schizosaccharomyces pombe MCC (a complex of mitotic spindle assembly checkpoint proteins Mad2, Mad3 and APC/C co-activator protein Cdc20), we reveal the molecular basis of MCC-mediated APC/C inhibition and the regulation of MCC assembly
      Title: Structure of the mitotic checkpoint complex.
    2. The Bub1 TPR domain is both necessary and sufficient to bind and recruit Mad3.
      Title: The Bub1-TPR Domain Interacts Directly with Mad3 to Generate Robust Spindle Checkpoint Arrest.
    3. A kinase-dead allele of the fission yeast MPS1 homolog (Mph1) is checkpoint defective and levels of anaphase promoting complex-associated Mad2 and Mad3 are dramatically reduced in this mutant.
      Title: Kinase activity of fission yeast Mph1 is required for Mad2 and Mad3 to stably bind the anaphase promoting complex.
    4. the Mad3 KEN box is a critical mediator of Cdc20-APC/C inhibition, without which neither Mad3 nor Mad2 can associate with the APC/C or inhibit anaphase onset
      Title: The spindle checkpoint functions of Mad3 and Mad2 depend on a Mad3 KEN box-mediated interaction with Cdc20-anaphase-promoting complex (APC/C).
    5. Bub1p-A78V is defective in both nuclear accumulation and kinetochore targeting; a threshold level of nuclear Bub1p is necessary for the nuclear accumulation of Bub3p and Mad3p
      Title: The A78V mutation in the Mad3-like domain of Schizosaccharomyces pombe Bub1p perturbs nuclear accumulation and kinetochore targeting of Bub1p, Bub3p, and Mad3p and spindle assembly checkpoint function.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by PomBase

    Function Evidence Code Pubs
    enables anaphase-promoting complex binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables ubiquitin ligase inhibitor activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in mitotic spindle assembly checkpoint signaling IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in mitotic spindle assembly checkpoint signaling IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of mitotic metaphase/anaphase transition IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in cytosol HDA PubMed 
    is_active_in kinetochore NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    part_of mitotic checkpoint complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in nucleus HDA PubMed 
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  

    General protein information

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    Preferred Names
    mitotic spindle checkpoint protein Mad3

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003421.2 Reference assembly

      Range
      997416..998839
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001023035.3 → NP_588043.2  mitotic spindle checkpoint protein Mad3 [Schizosaccharomyces pombe]

      See identical proteins and their annotated locations for NP_588043.2

      Status: PROVISIONAL

      UniProtKB/Swiss-Prot
      O59767
      Conserved Domains (1) summary
      smart00777
      Location:52 → 176
      Mad3_BUB1_I; Mad3/BUB1 hoMad3/BUB1 homology region 1
    Nucleotide Protein
    Heading Accession and Version

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