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    SEC17 Sec17p [ Saccharomyces cerevisiae S288C ]

    Gene ID: 852230, updated on 28-Oct-2024

    Summary

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    Official Symbol
    SEC17
    Official Full Name
    Sec17p
    Primary source
    SGD:S000000146
    Locus tag
    YBL050W
    See related
    AllianceGenome:SGD:S000000146; FungiDB:YBL050W; VEuPathDB:YBL050W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    RNS3
    Summary
    Enables ATPase activator activity and soluble NSF attachment protein activity. Involved in SNARE complex disassembly; autophagy; and organelle fusion. Located in cytosol. Part of SNARE complex. Human ortholog(s) of this gene implicated in developmental and epileptic encephalopathy 107. Orthologous to several human genes including NAPB (NSF attachment protein beta). [provided by Alliance of Genome Resources, Oct 2024]
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    Genomic context

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    See SEC17 in Genome Data Viewer
    Location:
    chromosome: II
    Exon count:
    2
    Sequence:
    Chromosome: II; NC_001134.8 (125125..126119)

    Chromosome II - NC_001134.8Genomic Context describing neighboring genes Neighboring gene histone acetyltransferase Neighboring gene Pin4p Neighboring gene Moh1p Neighboring gene Rrt1p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001134.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Biosynthesis of mannosylinositolphosphoceramide in Saccharomyces cerevisiae is dependent on genes controlling the flow of secretory vesicles from the endoplasmic reticulum to the Golgi. Puoti A, et al. J Cell Biol, 1991 May. PMID 2016333, Free PMC Article
    2. SNAPs, a family of NSF attachment proteins involved in intracellular membrane fusion in animals and yeast. Clary DO, et al. Cell, 1990 May 18. PMID 2111733
    3. Derepression of high-affinity glucose uptake requires a functional secretory system in Saccharomyces cerevisiae. Bisson LF. J Bacteriol, 1988 Jun. PMID 3286616, Free PMC Article
    4. A TPR domain in the SNAP secretory proteins. Ordway RW, et al. Trends Biochem Sci, 1994 Dec. PMID 7846761
    5. Inhibition of endoplasmic reticulum (ER)-to-Golgi transport induces relocalization of binding protein (BiP) within the ER to form the BiP bodies. Nishikawa S, et al. Mol Biol Cell, 1994 Oct. PMID 7865879, Free PMC Article

    See all (139) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Fusion with wild-type SNARE domains is controlled by juxtamembrane domains, transmembrane anchors, and Sec17.
      Title: Fusion with wild-type SNARE domains is controlled by juxtamembrane domains, transmembrane anchors, and Sec17.
    2. Fusion of tethered membranes can be driven by Sec18/NSF and Sec17/alphaSNAP without HOPS.
      Title: Fusion of tethered membranes can be driven by Sec18/NSF and Sec17/αSNAP without HOPS.
    3. SNARE-only fusion is abolished by Sec17 and Sec18. Efficient fusion with Sec17 and Sec18 requires a tripartite match between the organellar identities of the R-SNARE, the Q-SNAREs, and the SM protein/complex. Sec17 and Sec18 are not simply negative regulators; they stimulate fusion with either vacuolar SNAREs and their SM protein complex HOPS or endoplasmic reticulum/cis-Golgi SNAREs and their SM protein Sly1.
      Title: Sec17 (α-SNAP) and Sec18 (NSF) restrict membrane fusion to R-SNAREs, Q-SNAREs, and SM proteins from identical compartments.
    4. When SNAREs are more disassembled, as by Sec17, Sec18, and ATP (adenosine triphosphate), HOPS is required, and GST-PX does not suffice.
      Title: Tethering guides fusion-competent trans-SNARE assembly.
    5. Thus, Vps33/HOPS promotes productive SNARE assembly in the presence of otherwise inhibitory Sec17.
      Title: Sec17 (α-SNAP) and an SM-tethering complex regulate the outcome of SNARE zippering in vitro and in vivo.
    6. Sec17 and Sec18 act twice in the fusion cycle, binding to trans-SNARE complexes to accelerate fusion, then hydrolyzing ATP to disassemble cis-SNARE complexes.
      Title: Sec17/Sec18 act twice, enhancing membrane fusion and then disassembling cis-SNARE complexes.
    7. Sec17 binds to trans-SNARE complexes, oligomerizes, and inserts apolar loops into the apposed membranes, locally disturbing the lipid bilayer and lowering the energy barrier for fusion without Sec18
      Title: Sec17 can trigger fusion of trans-SNARE paired membranes without Sec18.
    8. Using a reconstituted proteoliposome fusion system, the authors show that trans-SNARE complex, like cis-SNARE complex, is sensitive to Sec17p/Sec18p mediated disassembly.
      Title: HOPS prevents the disassembly of trans-SNARE complexes by Sec17p/Sec18p during membrane fusion.
    9. Data show that the ternary synergy of phosphoinositides and 2 SNARE chaperone systems (SNARE Vam7p and Sec17p/Sec18p/ATP
      Title: Phosphoinositides and SNARE chaperones synergistically assemble and remodel SNARE complexes for membrane fusion.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables ATPase activator activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables soluble NSF attachment protein activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables soluble NSF attachment protein activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables soluble NSF attachment protein activity IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables syntaxin binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Process Evidence Code Pubs
    involved_in SNARE complex disassembly IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in SNARE complex disassembly IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in autophagy IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in intracellular protein transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in intracellular protein transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in protein transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in vacuole fusion, non-autophagic IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in vesicle fusion with Golgi apparatus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in vesicle-mediated transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of SNARE complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of SNARE complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in fungal-type vacuole membrane HDA PubMed 
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    Sec17p
    NP_009503.1
    • Alpha-SNAP cochaperone; SNARE-complex adaptor for Sec18 (NSF) during the disassembly of postfusion cis-SNARE complexes; stimulates the ATPase activity of Sec18p; peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, and autophagy; similar to mammalian alpha-SNAP

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001134.8 Reference assembly

      Range
      125125..126119
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001178290.1 → NP_009503.1  TPA: Sec17p [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_009503.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VPU9, P32602
      UniProtKB/TrEMBL
      A6ZKP8, B3LNI7, C7GNB4, C8Z3P9, G2W8V8, N1PAX3
      Conserved Domains (1) summary
      pfam14938
      Location:10 → 284
      SNAP; Soluble NSF attachment protein, SNAP
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P32602.4

    Gene LinkOut

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