BUD27 Bud27p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: BUD27
Official Full Name: Bud27p
Primary source: SGD:S000001871
Locus tag: YFL023W
See related: AllianceGenome:SGD:S000001871; FungiDB:YFL023W; VEuPathDB:YFL023W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: URI1
Summary: Enables mRNA binding activity. Involved in protein-containing complex assembly. Acts upstream of or within with a positive effect on 5S class rRNA transcription by RNA polymerase III and tRNA transcription by RNA polymerase III. Acts upstream of or within positive regulation of transcription elongation by RNA polymerase I and rRNA processing. Located in cytoplasm. [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See BUD27 in Genome Data Viewer

Location:: chromosome: VI

Exon count:: 1

Sequence:: Chromosome: VI; NC_001138.5 (90986..93376)

Chromosome VI - NC_001138.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001138.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The association of the RSC remodeler complex with chromatin is influenced by the prefoldin-like Bud27 and determines nucleosome positioning and polyadenylation sites usage in Saccharomyces cerevisiae.
Title: The association of the RSC remodeler complex with chromatin is influenced by the prefoldin-like Bud27 and determines nucleosome positioning and polyadenylation sites usage in Saccharomyces cerevisiae.
A High-Copy Suppressor Screen Reveals a Broad Role of Prefoldin-like Bud27 in the TOR Signaling Pathway in Saccharomyces cerevisiae.
Title: A High-Copy Suppressor Screen Reveals a Broad Role of Prefoldin-like Bud27 in the TOR Signaling Pathway in Saccharomyces cerevisiae.
Yeast Bud27 modulates the biogenesis of Rpc128 and Rpc160 subunits and the assembly of RNA polymerase III.
Title: Yeast Bud27 modulates the biogenesis of Rpc128 and Rpc160 subunits and the assembly of RNA polymerase III.
The data suggest that Bud27 contributes to the folding of Rpb5 within the RNA polymerase II thereby modulating its activity, and participates in the correct assembly of other chromatin-associated factors, such as the RSC complex.
Title: The yeast prefoldin-like URI-orthologue Bud27 associates with the RSC nucleosome remodeler and modulates transcription.
Our data also suggest that the role of Bud27 in RNA pols biogenesis is independent of the chaperone prefoldin (PFD) complex and of Iwr1
Title: The prefoldin bud27 mediates the assembly of the eukaryotic RNA polymerases in an rpb5-dependent manner.
Together with genetic data, these interactions indicate that Uri1p may coordinate translation initiation and cotranslational quality control.
Title: Yeast Uri1p promotes translation initiation and may provide a link to cotranslational quality control.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables mRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables unfolded protein binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within_positive_effect 5S class rRNA transcription by RNA polymerase III	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in RNA polymerase I assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in RNA polymerase I assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in RNA polymerase II core complex assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in RNA polymerase II core complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in RNA polymerase III assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in RNA polymerase III assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in formation of translation preinitiation complex	IGI Inferred from Genetic Interaction more info	PubMed
involved_in formation of translation preinitiation complex	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of transcription elongation by RNA polymerase I	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein folding	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within rRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within_positive_effect tRNA transcription by RNA polymerase III	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
part_of prefoldin complex	IBA Inferred from Biological aspect of Ancestor more info
part_of prefoldin complex	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Bud27p

NP_683715.1

Unconventional prefoldin protein involved in translation initiation; required for correct assembly of RNAP I, II, and III in an Rpb5p-dependent manner; shuttles between nucleus and cytoplasm; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; mutations affect TOR signaling; ortholog of human URI/RMP

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001138.5 Reference assembly

Range

90986..93376

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179943.1 → NP_683715.1 TPA: Bud27p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_683715.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VTK7, P43573

UniProtKB/TrEMBL

C7GY88

Conserved Domains (2) summary

pfam02996
Location:8 → 121

Prefoldin; Prefoldin subunit

pfam12927
Location:714 → 788

DUF3835; Domain of unknown function (DUF3835)