GLN3 nitrogen-responsive transcriptional regulator GLN3 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: GLN3
Official Full Name: nitrogen-responsive transcriptional regulator GLN3
Primary source: SGD:S000000842
Locus tag: YER040W
See related: AllianceGenome:SGD:S000000842; FungiDB:YER040W; VEuPathDB:YER040W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables DNA-binding transcription factor activity and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Involved in regulation of protein localization by the Cvt pathway and regulation of transcription by RNA polymerase II. Located in cytosol and nucleus. Human ortholog(s) of this gene implicated in several diseases, including Down syndrome; X-linked recessive disease (multiple); adenocarcinoma (multiple); congenital heart disease (multiple); and hematologic cancer (multiple). Orthologous to several human genes including GATA1 (GATA binding protein 1); GATA2 (GATA binding protein 2); and GATA3 (GATA binding protein 3). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See GLN3 in Genome Data Viewer

Location:: chromosome: V

Exon count:: 1

Sequence:: Chromosome: V; NC_001137.3 (229795..231987)

Chromosome V - NC_001137.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001137.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

N- and C-terminal Gln3-Tor1 interaction sites: one acting negatively and the other positively to regulate nuclear Gln3 localization.
Title: N- and C-terminal Gln3-Tor1 interaction sites: one acting negatively and the other positively to regulate nuclear Gln3 localization.
Aminoacyl-tRNA quality control is required for efficient activation of the TOR pathway regulator Gln3p.
Title: Aminoacyl-tRNA quality control is required for efficient activation of the TOR pathway regulator Gln3p.
Performed a genome-wide screen in Saccharomyces cerevisiae to identify genes linked to high-fidelity DNA replication. Found URA7 or GLN3 inactivation had a role in imbalanced dNTP pools and increased mutagenesis.
Title: Alterations in cellular metabolism triggered by URA7 or GLN3 inactivation cause imbalanced dNTP pools and increased mutagenesis.
two different ways in which intracellular Gln3 localization is regulated
Title: Nuclear Gln3 Import Is Regulated by Nitrogen Catabolite Repression Whereas Export Is Specifically Regulated by Glutamine.
nuclear localization domains of GATA activator Gln3 are required for transcription of target genes, presumably through dephosphorylation
Title: Nuclear localization domains of GATA activator Gln3 are required for transcription of target genes through dephosphorylation in Saccharomyces cerevisiae.
we conclude that Siw14 functions upstream of Pph21 and Pph22 as an inhibitor of the phosphorylation and localization of Gln3, and that Sit4 acts independently of Siw14
Title: The protein phosphatase Siw14 controls caffeine-induced nuclear localization and phosphorylation of Gln3 via the type 2A protein phosphatases Pph21 and Pph22 in Saccharomyces cerevisiae.
Rapamycin-dependent Gat1 and Gln3 binding to the Aro80 target promoters is not affected by tryptophan availability, suggesting that transactivation activity of Aro80 is not necessary for the recruitment of GATA factors.
Title: Interplay of Aro80 and GATA activators in regulation of genes for catabolism of aromatic amino acids in Saccharomyces cerevisiae.
gln3 mutations dissociate responses to nitrogen limitation (nitrogen catabolite repression) and rapamycin inhibition of TorC1
Title: gln3 mutations dissociate responses to nitrogen limitation (nitrogen catabolite repression) and rapamycin inhibition of TorC1.
Gln3, Gat1, and Ure2 respond to TorC1 and nitrogen availability via distinct regulatory pathways.
Title: Alterations in the Ure2 αCap domain elicit different GATA factor responses to rapamycin treatment and nitrogen limitation.
Nitrogen-responsive regulation of GATA protein family activators Gln3 and Gat1 occurs by two distinct pathways, one inhibited by rapamycin and the other by methionine sulfoximine.
Title: Nitrogen-responsive regulation of GATA protein family activators Gln3 and Gat1 occurs by two distinct pathways, one inhibited by rapamycin and the other by methionine sulfoximine.

Submit: New GeneRIF Correction See all GeneRIFs (26)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables sequence-specific DNA binding	HDA more info	PubMed
enables sequence-specific DNA binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in negative regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in nitrate assimilation	IEA Inferred from Electronic Annotation more info
involved_in nitrogen catabolite activation of transcription from RNA polymerase II promoter	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: nitrogen-responsive transcriptional regulator GLN3

NP_010958.3

Transcriptional activator in nitrogen catabolite repression system; localization and activity regulated by quality of nitrogen source and Ure2p; full-length protein forms prion-like aggregates when overproduced

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001137.3 Reference assembly

Range

229795..231987

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178931.3 → NP_010958.3 TPA: nitrogen-responsive transcriptional regulator GLN3 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010958.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DLU0, P18494

UniProtKB/TrEMBL

C7GSI7, C8Z743

Conserved Domains (1) summary

COG5641
Location:56 → 715

GAT1; GATA Zn-finger-containing transcription factor [Transcription]