PHO89 Pho89p [Saccharomyces cerevisiae S288C] - Gene

Summary

Official Symbol: PHO89
Official Full Name: Pho89p
Primary source: SGD:S000000500
Locus tag: YBR296C
See related: AllianceGenome:SGD:S000000500; FungiDB:YBR296C; VEuPathDB:YBR296C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: ITN1
Summary: Enables sodium:phosphate symporter activity. Involved in phosphate ion transmembrane transport. Located in plasma membrane. Human ortholog(s) of this gene implicated in basal ganglia calcification. Orthologous to human SLC20A1 (solute carrier family 20 member 1) and SLC20A2 (solute carrier family 20 member 2). [provided by Alliance of Genome Resources, Oct 2024]
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Chromosome II - NC_001134.8 Genomic Context describing neighboring genes

Go to nucleotide: Graphics FASTA GenBank

Data highlight a scenario in which the activities of Pho89 and Ena1 are functionally coordinated to sustain growth in an alkaline environment.
Title: Coregulated expression of the Na+/phosphate Pho89 transporter and Ena1 Na+-ATPase allows their functional coupling under high-pH stress.
Pho89 protein undergoes conformational change upon addition of substrate.
Title: Characterization of the biochemical and biophysical properties of the Saccharomyces cerevisiae phosphate transporter Pho89.
Data indicate that purified and reconstituted Pho89 showed Na(+)-dependent Pi transport activity driven by an artificially imposed electrochemical Na(+) gradient.
Title: Functional expression, purification and reconstitution of the recombinant phosphate transporter Pho89 of Saccharomyces cerevisiae.
Even if Pho89 is synthesized in cells grown at pH 4.5-8.0, the transporter is functionally active under alkaline conditions only
Title: Characterization of the Pho89 phosphate transporter by functional hyperexpression in Saccharomyces cerevisiae.

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

Function	Evidence Code	Pubs
enables phosphate transmembrane transporter activity	IEA Inferred from Electronic Annotation more info
enables sodium:phosphate symporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables sodium:phosphate symporter activity	IDA Inferred from Direct Assay more info	PubMed
enables sodium:phosphate symporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables symporter activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in phosphate ion transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in phosphate ion transmembrane transport	IDA Inferred from Direct Assay more info	PubMed
involved_in phosphate ion transport	IEA Inferred from Electronic Annotation more info
involved_in phosphate ion transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sodium ion transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in transmembrane transport	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in endoplasmic reticulum	HDA more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IMP Inferred from Mutant Phenotype more info	PubMed

NP_009855.1

Plasma membrane Na+/Pi cotransporter; active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification