USP20 ubiquitin specific peptidase 20 [Homo sapiens (human)] - Gene

Summary

Official Symbol: USP20provided by HGNC
Official Full Name: ubiquitin specific peptidase 20provided by HGNC
Primary source: HGNC:HGNC:12619
See related: Ensembl:ENSG00000136878 MIM:615143; AllianceGenome:HGNC:12619
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: VDU2; hVDU2; LSFR3A
Summary: This gene encodes a ubiquitin specific processing protease that was first identified as a substrate of the VHL (von Hippel-Lindau disease) protein E3 ubiquitin ligase complex. In addition to being ubiquitinated by the VHL-E3 ligase complex, this enzyme deubiquitinates hypoxia-inducible factor (HIF)-1 alpha and thereby causes increased expression of HIF-1alpha targeted genes which play a role in angiogenesis, glucose metabolism, cell proliferation and metastasis. The enzyme encoded by this gene also regulates G-protein coupled receptor signaling by mediating the deubiquitination of beta-2 adrenergic receptor (ADRB2). This enzyme is a ubiquitously expressed thiolester hydrolase. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jan 2013]
Expression: Ubiquitous expression in lymph node (RPKM 8.9), bone marrow (RPKM 8.3) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 9q34.11

Exon count:: 26

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	9	NC_000009.12 (129835458..129881828)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	9	NC_060933.1 (142040029..142086556)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	9	NC_000009.11 (132597737..132644107)

Chromosome 9 - NC_000009.12 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Phosphorylation of USP20 on Ser334 by IRAK1 promotes IL-1beta-evoked signaling in vascular smooth muscle cells and vascular inflammation.
Title: Phosphorylation of USP20 on Ser334 by IRAK1 promotes IL-1β-evoked signaling in vascular smooth muscle cells and vascular inflammation.
Expression, purification and characterization of the second DUSP domain of deubiquitinase USP20/VDU2.
Title: Expression, purification and characterization of the second DUSP domain of deubiquitinase USP20/VDU2.
Deubiquitinases USP20/33 promote the biogenesis of tail-anchored membrane proteins.
Title: Deubiquitinases USP20/33 promote the biogenesis of tail-anchored membrane proteins.
Deubiquitinase USP20 promotes breast cancer metastasis by stabilizing SNAI2.
Title: Deubiquitinase USP20 promotes breast cancer metastasis by stabilizing SNAI2.
The Deubiquitinating Enzyme USP20 Regulates the TNFalpha-Induced NF-kappaB Signaling Pathway through Stabilization of p62.
Title: The Deubiquitinating Enzyme USP20 Regulates the TNFα-Induced NF-κB Signaling Pathway through Stabilization of p62.
Study revealed the molecular basis for the weak binding of USP20 N-terminal zinc-finger ubiquitin binding domain (ZnF-UBP) to mono-ubiquitin and poly-ubiquitin. The solution structure of USP20 ZnF-UBP domain consists of a central beta-sheet with either one alpha-helix or two alpha-helices packed on each side of the sheet. The overall structure of USP20 ZnF-UBP domain is stabilized by three coordinated zinc ions.
Title: Structural and functional studies of USP20 ZnF-UBP domain by NMR.
USP20 is a positive regulator of beta-catenin in cancer cell migration, invasion and tumor growth in vivo. Mechanistically, USP20 deubiquitinates and stabilizes beta-catenin and is a potential biomarker for predicting chemoresistance in cancer.
Title: USP20 positively regulates tumorigenesis and chemoresistance through β-catenin stabilization.
our results demonstrate a role of the USP20 deubiquitinating enzyme in acting as a critical regulator of ULK1 and show a novel mechanism of the autophagy process in terms of ULK1 stability.
Title: The deubiquitinating enzyme USP20 stabilizes ULK1 and promotes autophagy initiation.
USP20 regulates actin cytoskeleton organization and cell migration in a manner dependent on ERK3 expression. The results identify USP20 as a bona fide regulator of ERK3 stability and physiological functions.
Title: Deubiquitinating Enzyme USP20 Regulates Extracellular Signal-Regulated Kinase 3 Stability and Biological Activity.
we investigated the inter-action between PKM2 and USP20. Our results suggest a new molecular pathway in cancer metabolism through the regulation of PKM2.
Title: Regulation of pyruvate kinase isozyme M2 is mediated by the ubiquitin-specific protease 20.

Submit: New GeneRIF Correction See all GeneRIFs (18)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Replication interactions

Interaction	Pubs
Knockdown of ubiquitin specific peptidase 20 (USP20) by siRNA inhibits HIV-1 replication in HeLa P4/R5 cells	PubMed

Protein interactions

Protein	Gene	Interaction	Pubs
Tat	tat	Expression of HIV-1 Tat downregulates the abundance of ubiquitin specific peptidase 20 (USP20) in the nucleoli of Jurkat T-cells	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

SHGC-147266 (e-PCR)
- UniSTS:175707

RH12701 (e-PCR)
- UniSTS:90490

D9S1766 (e-PCR)
- UniSTS:154238

SHGC-58438 (e-PCR)
- UniSTS:71903

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables G protein-coupled receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables cysteine-type deubiquitinase activity	IDA Inferred from Direct Assay more info	PubMed
enables cysteine-type deubiquitinase activity	TAS Traceable Author Statement more info
enables cysteine-type endopeptidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in antiviral innate immune response	IDA Inferred from Direct Assay more info	PubMed
involved_in endocytosis	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of canonical NF-kappaB signal transduction	IDA Inferred from Direct Assay more info	PubMed
involved_in nervous system development	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of autophagy	IDA Inferred from Direct Assay more info	PubMed
involved_in protein K48-linked deubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein K63-linked deubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein deubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein deubiquitination	TAS Traceable Author Statement more info
involved_in proteolysis	IEA Inferred from Electronic Annotation more info
involved_in regulation of G protein-coupled receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of G protein-coupled receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in centrosome	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IC Inferred by Curator more info	PubMed
located_in cytosol	TAS Traceable Author Statement more info
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: ubiquitin carboxyl-terminal hydrolase 20

Names: deubiquitinating enzyme 20; pVHL-interacting deubiquitinating enzyme 2; ubiquitin thioesterase 20; ubiquitin thiolesterase 20; ubiquitin-specific-processing protease 20

NP_001008563.2

EC 3.4.19.12

NP_001103773.2

EC 3.4.19.12

NP_006667.3

EC 3.4.19.12

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

NG_033097.2 RefSeqGene

Range

5030..51400

Download

GenBank, FASTA, Sequence Viewer (Graphics), LRG_711

mRNA and Protein(s)

NM_001008563.5 → NP_001008563.2 ubiquitin carboxyl-terminal hydrolase 20

See identical proteins and their annotated locations for NP_001008563.2

Status: REVIEWED

Description

Transcript Variant: This variant (2) has different 5' and 3' non-coding, terminal exons, compared to transcript variant 1. All three transcript variants encode the same protein.

Source sequence(s)

AL158207

Consensus CDS

CCDS43892.1

UniProtKB/Swiss-Prot

Q541F1, Q8IXQ1, Q96LG5, Q9UQN8, Q9UQP0, Q9Y2K6

Related

ENSP00000351122.1, ENST00000358355.5

Conserved Domains (5) summary

smart00695
Location:810 → 895

DUSP; Domain in ubiquitin-specific proteases

cd02674
Location:443 → 683

Peptidase_C19R; A subfamily of peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...

cl02553
Location:144 → 291

Peptidase_C19; Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyse bonds involving the carboxyl group of the C-terminal Gly ...

pfam00443
Location:144 → 682

UCH; Ubiquitin carboxyl-terminal hydrolase

pfam02148
Location:30 → 90

zf-UBP; Zn-finger in ubiquitin-hydrolases and other protein
NM_001110303.4 → NP_001103773.2 ubiquitin carboxyl-terminal hydrolase 20

See identical proteins and their annotated locations for NP_001103773.2

Status: REVIEWED

Description

Transcript Variant: This variant (3) has a different 3' non-coding, terminal exon, compared to transcript variant 1. All three transcript variants encode the same protein.

Source sequence(s)

AL158207

Consensus CDS

CCDS43892.1

UniProtKB/Swiss-Prot

Q541F1, Q8IXQ1, Q96LG5, Q9UQN8, Q9UQP0, Q9Y2K6

Related

ENSP00000361506.3, ENST00000372429.8

Conserved Domains (5) summary

smart00695
Location:810 → 895

DUSP; Domain in ubiquitin-specific proteases

cd02674
Location:443 → 683

Peptidase_C19R; A subfamily of peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...

cl02553
Location:144 → 291

Peptidase_C19; Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyse bonds involving the carboxyl group of the C-terminal Gly ...

pfam00443
Location:144 → 682

UCH; Ubiquitin carboxyl-terminal hydrolase

pfam02148
Location:30 → 90

zf-UBP; Zn-finger in ubiquitin-hydrolases and other protein
NM_006676.8 → NP_006667.3 ubiquitin carboxyl-terminal hydrolase 20

See identical proteins and their annotated locations for NP_006667.3

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longest transcript. All three transcript variants encode the same protein.

Source sequence(s)

AL158207

Consensus CDS

CCDS43892.1

UniProtKB/Swiss-Prot

Q541F1, Q8IXQ1, Q96LG5, Q9UQN8, Q9UQP0, Q9Y2K6

Related

ENSP00000313811.4, ENST00000315480.9

Conserved Domains (5) summary

smart00695
Location:810 → 895

DUSP; Domain in ubiquitin-specific proteases

cd02674
Location:443 → 683

Peptidase_C19R; A subfamily of peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...

cl02553
Location:144 → 291

Peptidase_C19; Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyse bonds involving the carboxyl group of the C-terminal Gly ...

pfam00443
Location:144 → 682

UCH; Ubiquitin carboxyl-terminal hydrolase

pfam02148
Location:30 → 90

zf-UBP; Zn-finger in ubiquitin-hydrolases and other protein