UBC5 E2 ubiquitin-conjugating protein UBC5 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: UBC5
Official Full Name: E2 ubiquitin-conjugating protein UBC5
Primary source: SGD:S000002466
Locus tag: YDR059C
See related: AllianceGenome:SGD:S000002466; FungiDB:YDR059C; VEuPathDB:YDR059C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables proteasome binding activity and ubiquitin-protein transferase activity. Involved in cellular response to heat and protein polyubiquitination. Predicted to be part of ubiquitin ligase complex. Predicted to be active in nucleus. Orthologous to human UBE2D1 (ubiquitin conjugating enzyme E2 D1). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See UBC5 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 2

Sequence:: Chromosome: IV; NC_001136.10 (569234..569770, complement)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Together, these data point to an important role for Tom1, Ubc4 and Ubc5 in normal physiology and prompt the authors to refer to this pathway as ERISQ, for excess ribosomal protein quality control.
Title: A conserved quality-control pathway that mediates degradation of unassembled ribosomal proteins.
Ubc4 and Ubc5 are 1) the primary E2s for Rsp5 in yeast and 2) act as monoubiquitinating E2s in RING E3-catalyzed pathways
Title: The essential Ubc4/Ubc5 function in yeast is HECT E3-dependent, and RING E3-dependent pathways require only monoubiquitin transfer by Ubc4.
Data demonstrated that excess histones associate with Rad53, and also identified Ubc4 and Ubc5, as well as Tom1 as enzymes involved in the ubiquitylation of excess histones.
Title: Histone levels are regulated by phosphorylation and ubiquitylation-dependent proteolysis.
These results show that Not4p functions as an E3 ligase by modulating Ubc4p/Ubc5p-mediated stress responses in vivo.
Title: Modulation of Ubc4p/Ubc5p-mediated stress responses by the RING-finger-dependent ubiquitin-protein ligase Not4p in Saccharomyces cerevisiae.
An Rsp5 dimer assembled on the RNA polymerase II (RNAP II) C-terminal domain; ubiquitylation sites bind Ubc5, which in turn binds Rsp5 to allow modification of RPB1 subunit of RNAP II.
Title: Communication between distant sites in RNA polymerase II through ubiquitylation factors and the polymerase CTD.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables proteasome binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin conjugating enzyme activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin conjugating enzyme activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin-protein transferase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in protein polyubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein polyubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
is_active_in cellular_component	ND No biological Data available more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: E2 ubiquitin-conjugating protein UBC5

NP_010344.1

EC 2.3.2.23

Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

569234..569770 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180367.1 → NP_010344.1 TPA: E2 ubiquitin-conjugating protein UBC5 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010344.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VS45, P15732

UniProtKB/TrEMBL

A6ZY24, B3LGL0, C8Z4X8, G2WAG3, N1PAC4

Conserved Domains (1) summary

cl00154
Location:3 → 147

UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...