EDE1 Ede1p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: EDE1
Official Full Name: Ede1p
Primary source: SGD:S000000143
Locus tag: YBL047C
See related: AllianceGenome:SGD:S000000143; FungiDB:YBL047C; VEuPathDB:YBL047C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: BUD15
Summary: Enables ubiquitin binding activity. Involved in several processes, including actin cortical patch organization; endoplasmic reticulum unfolded protein response; and positive regulation of cytokinesis. Located in actin cortical patch; cellular bud neck; and cellular bud tip. Orthologous to human EPS15 (epidermal growth factor receptor pathway substrate 15) and EPS15L1 (epidermal growth factor receptor pathway substrate 15 like 1). [provided by Alliance of Genome Resources, Oct 2024]
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Genomic context

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See EDE1 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 1

Sequence:: Chromosome: II; NC_001134.8 (127895..132040, complement)

Chromosome II - NC_001134.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The conserved protein adaptors CALM/AP180 and FCHo1/2 cooperatively recruit Eps15 to promote the initiation of clathrin-mediated endocytosis in yeast.
Title: The conserved protein adaptors CALM/AP180 and FCHo1/2 cooperatively recruit Eps15 to promote the initiation of clathrin-mediated endocytosis in yeast.
Condensation of Ede1 promotes the initiation of endocytosis.
Title: Condensation of Ede1 promotes the initiation of endocytosis.
When expressed in yeast, human Eps15 localized to the plasma membrane, where it recruited late-phase CME proteins and supported productive endocytosis, identifying it as an Ede1 functional homologue.
Title: Selection and stabilization of endocytic sites by Ede1, a yeast functional homologue of human Eps15.
Hrr25 kinase and Ede1 cooperate to initiate and stabilize endocytic sites
Title: Casein kinase 1 promotes initiation of clathrin-mediated endocytosis.
a new and widely applicable tool to generate conditionally phosphorylated proteins to identify those whose dephosphorylation is required for cytokinesis. This approach identifies Aip1, Ede1 and Inn1 as cytokinetic regulators.
Title: Identification of Cdk targets that control cytokinesis.
Results show that oligomerization of Ede1 is essential for its localization and function as an early endocytic scaffold protein.
Title: Quantification of cytosolic interactions identifies Ede1 oligomers as key organizers of endocytosis.
Deubiquitination regulates formation of endocytic sites and stability of the endocytic coat and occurs through Ede1, because permanently ubiquitinated Ede1 phenocopies deletion of UBP2 and UBP7.
Title: Regulation of clathrin-mediated endocytosis by dynamic ubiquitination and deubiquitination.
Results suggest that Ede1p and Syp1p are conserved, early-arriving endocytic proteins with roles in the formation and placement of endocytic sites, respectively.
Title: Early-arriving Syp1p and Ede1p function in endocytic site placement and formation in budding yeast.
Epsin/Yap180 adaptor interaction with the scaffolds Ede1/Pan1 is required for endocytosis
Title: Interaction between Epsin/Yap180 adaptors and the scaffolds Ede1/Pan1 is required for endocytosis.
Ede1 ubiquitin associated domain-ubiquitin interaction reveal several key affinity determinants and, unexpectedly, a negative affinity determinant in the wild-type Ede1 protein
Title: Structural basis for monoubiquitin recognition by the Ede1 UBA domain.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables ubiquitin binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in actin cortical patch organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in endocytosis	IEA Inferred from Electronic Annotation more info
involved_in endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endoplasmic reticulum unfolded protein response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endosomal transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of cytokinesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of protein localization	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in actin cortical patch	IDA Inferred from Direct Assay more info	PubMed
located_in cellular bud neck	HDA more info	PubMed
located_in cellular bud neck	IDA Inferred from Direct Assay more info	PubMed
located_in cellular bud tip	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in mating projection tip	HDA more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: Ede1p

NP_009506.1

Endocytic adaptor; K63-specific Ub-chain binding protein involved in receptor endocytosis; scaffold protein that localizes to nascent endocytic sites after homo-oligomerization and organizes the endocytic machinery; role in autophagy of aberrant clathrin-mediated endocytosis protein condensates; binds membranes in a Ub-dependent manner; interacts with and functions upstream of Cmk2p to suppress stress-induced non-apoptotic cell death; putative cytokinesis regulator; homolog of mammalian Eps15

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001134.8 Reference assembly

Range

127895..132040 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178287.1 → NP_009506.1 TPA: Ede1p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009506.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VPV1, P34216

UniProtKB/TrEMBL

A6ZKQ0, C7GNB6, G2W8W0

Conserved Domains (5) summary

smart00027
Location:270 → 363

EH; Eps15 homology domain

COG1196
Location:595 → 877

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

PRK08581
Location:903 → 1111

PRK08581; amidase domain-containing protein

cd14285
Location:1345 → 1379

UBA_scEDE1_like; UBA domain found in Saccharomyces cerevisiae EH domain-containing and endocytosis protein 1 (Ede1) and similar proteins

pfam03154
Location:355 → 528

Atrophin-1; Atrophin-1 family