THI3 branched-chain-2-oxoacid decarboxylase THI3 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: THI3
Official Full Name: branched-chain-2-oxoacid decarboxylase THI3
Primary source: SGD:S000002238
Locus tag: YDL080C
See related: AllianceGenome:SGD:S000002238; FungiDB:YDL080C; VEuPathDB:YDL080C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: KID1
Summary: Enables RNA polymerase II-specific DNA-binding transcription factor binding activity. Involved in positive regulation of thiamine biosynthetic process and positive regulation of transcription by RNA polymerase II. Located in nucleus. [provided by Alliance of Genome Resources, Oct 2024]
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Genomic context

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See THI3 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (310642..312471, complement)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

THI3 contributes to isoamyl alcohol biosynthesis through thiamine diphosphate homeostasis.
Title: THI3 contributes to isoamyl alcohol biosynthesis through thiamine diphosphate homeostasis.
Thi3p causes a conformational change in Pdc2p leading to full transactivation activity under favorable conditions.
Title: Thiamin-dependent transactivation activity of PDC2 in Saccharomyces cerevisiae.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables RNA polymerase II-specific DNA-binding transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed
enables branched-chain-2-oxoacid decarboxylase activity	IEA Inferred from Electronic Annotation more info
enables carboxy-lyase activity	IEA Inferred from Electronic Annotation more info
enables magnesium ion binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables thiamine pyrophosphate binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in amino acid catabolic process via Ehrlich pathway	IEA Inferred from Electronic Annotation more info
involved_in branched-chain amino acid catabolic process	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of thiamine biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of thiamine biosynthetic process	IPI Inferred from Physical Interaction more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: branched-chain-2-oxoacid decarboxylase THI3

NP_010203.1

EC 4.1.1.72

Regulatory protein that binds Pdc2p and Thi2p transcription factors; activates thiamine biosynthesis transcription factors Pdc2p and Thi2p by binding to them, but releases and de-activates them upon binding to thiamine pyrophosphate (TPP), the end product of the pathway; has similarity to decarboxylases but enzymatic activity is not detected

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

310642..312471 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180139.1 → NP_010203.1 TPA: branched-chain-2-oxoacid decarboxylase THI3 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010203.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VRR9, P89098, Q07471

UniProtKB/TrEMBL

A6ZXP8, C8Z4J0, N1P457

Conserved Domains (1) summary

COG3961
Location:12 → 579

PDC1; TPP-dependent 2-oxoacid decarboxylase, includes indolepyruvate decarboxylase [Carbohydrate transport and metabolism, Coenzyme transport and metabolism, General function prediction only]