PAN1 Pan1p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: PAN1
Official Full Name: Pan1p
Primary source: SGD:S000001445
Locus tag: YIR006C
See related: AllianceGenome:SGD:S000001445; FungiDB:YIR006C; VEuPathDB:YIR006C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: DIM2; MDP3; MIP3
Summary: Enables Arp2/3 complex binding activity. Involved in several processes, including actin cortical patch assembly; cellular bud site selection; and positive regulation of Arp2/3 complex-mediated actin nucleation. Acts upstream of or within reticulophagy. Located in actin cortical patch and nucleus. Part of actin cytoskeleton-regulatory complex. Human ortholog(s) of this gene implicated in neurodegeneration with brain iron accumulation. Orthologous to human REPS1 (RALBP1 associated Eps domain containing 1). [provided by Alliance of Genome Resources, Oct 2024]
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Genomic context

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See PAN1 in Genome Data Viewer

Location:: chromosome: IX

Exon count:: 1

Sequence:: Chromosome: IX; NC_001141.2 (365466..369908, complement)

Chromosome IX - NC_001141.2 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001141.2

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Eps15/Pan1p is a master regulator of the late stages of the endocytic pathway.
Title: Eps15/Pan1p is a master regulator of the late stages of the endocytic pathway.
Cooperative regulation of endocytic vesicle transport by yeast Eps15-like protein Pan1p and epsins.
Title: Cooperative regulation of endocytic vesicle transport by yeast Eps15-like protein Pan1p and epsins.
TORC2-dependent phosphorylation of the coat protein Pan1 by Akl1 controls endocytosis dynamics in Saccharomyces cerevisiae.
Title: Target of rapamycin complex 2-dependent phosphorylation of the coat protein Pan1 by Akl1 controls endocytosis dynamics in Saccharomyces cerevisiae.
Thus Pan1 phosphorylation is part of a novel mechanism that regulates endocytic compartment interactions with each other and with actin cables.
Title: Yeast Eps15-like endocytic protein Pan1p regulates the interaction between endocytic vesicles, endosomes and the actin cytoskeleton.
A region of Pan1 spanning the second EH domain and central region was critical for viability and endocytosis. Other regions of Pan1 supported viability and not endocytosis, suggesting that Pan1 may have an additional role independent of endocytosis.
Title: Pan1 regulates transitions between stages of clathrin-mediated endocytosis.
N-terminal part of Pan1's central coiled-coil region binds End3 C-terminus.
Title: Targeted disruption of an EH-domain protein endocytic complex, Pan1-End3.
Pan1 contains functional nuclear localization signals (NLS) and a NES and appears to shuttle in certain circumstances
Title: Characterization of nuclear localization and nuclear export signals of yeast actin-binding protein Pan1.
Pan1p: an actin director of endocytosis in yeast.
Title: Pan1p: an actin director of endocytosis in yeast.
Pan1 and the type I myosins interactions promote an actin activity important at a late stage in endocytic internalization.
Title: Interaction of the endocytic scaffold protein Pan1 with the type I myosins contributes to the late stages of endocytosis.
Pan1p, and possibly other components of the actin cytoskeleton, are necessary to properly regulate growth of dividing cells in response to the presence of some alternative carbon sources (oleate) in the medium.
Title: Pan1p, an actin cytoskeleton-associated protein, is required for growth of yeast on oleate medium.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables Arp2/3 complex binding	IDA Inferred from Direct Assay more info	PubMed
enables actin binding	IEA Inferred from Electronic Annotation more info
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in actin cortical patch assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in axial cellular bud site selection	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in bipolar cellular bud site selection	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in endocytosis	IEA Inferred from Electronic Annotation more info
involved_in endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endosomal transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of Arp2/3 complex-mediated actin nucleation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within reticulophagy	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in actin cortical patch	IDA Inferred from Direct Assay more info	PubMed
located_in actin cortical patch	IEA Inferred from Electronic Annotation more info
part_of actin cytoskeleton-regulatory complex	IDA Inferred from Direct Assay more info	PubMed
part_of actin cytoskeleton-regulatory complex	IPI Inferred from Physical Interaction more info	PubMed
located_in cellular bud neck	HDA more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in endosome	IEA Inferred from Electronic Annotation more info
located_in endosome membrane	IEA Inferred from Electronic Annotation more info
located_in mating projection tip	HDA more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	HDA more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Pan1p

NP_012271.3

Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p; associates with actin patches on cell cortex; promotes protein-protein interactions essential for endocytosis; regulates late stages of endocytosis; binds to and activates Arp2/3 complex in vitro; phosphorylation of Thr-1225 is regulated by MAPK Hog1p in response to osmotic stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001141.2 Reference assembly

Range

365466..369908 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179528.3 → NP_012271.3 TPA: Pan1p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012271.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VVT6, P32521

UniProtKB/TrEMBL

C7GJG6, N1P975

Conserved Domains (4) summary

smart00027
Location:595 → 688

EH; Eps15 homology domain

pfam08226
Location:459 → 553

DUF1720; Domain of unknown function (DUF1720)

NF012221
Location:884 → 1033

MARTX_Nterm; MARTX multifunctional-autoprocessing repeats-in-toxin holotoxin N-terminal region

pfam02948
Location:1056 → 1125

Amelogenin