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    DGA1 diacylglycerol O-acyltransferase [ Saccharomyces cerevisiae S288C ]

    Gene ID: 854419, updated on 2-Nov-2024

    Summary

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    Official Symbol
    DGA1
    Official Full Name
    diacylglycerol O-acyltransferase
    Primary source
    SGD:S000005771
    Locus tag
    YOR245C
    See related
    AllianceGenome:SGD:S000005771; FungiDB:YOR245C; VEuPathDB:YOR245C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables diacylglycerol O-acyltransferase activity. Involved in several processes, including intracellular triglyceride homeostasis; lipid storage; and triglyceride biosynthetic process. Located in lipid droplet and membrane. Human ortholog(s) of this gene implicated in metabolic dysfunction-associated steatotic liver disease. Orthologous to several human genes including MOGAT3 (monoacylglycerol O-acyltransferase 3). [provided by Alliance of Genome Resources, Nov 2024]
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    Genomic context

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    See DGA1 in Genome Data Viewer
    Location:
    chromosome: XV
    Exon count:
    1
    Sequence:
    Chromosome: XV; NC_001147.6 (794076..795332, complement)

    Chromosome XV - NC_001147.6Genomic Context describing neighboring genes Neighboring gene pseudouridine synthase PUS7 Neighboring gene NuA4 histone acetyltransferase complex catalytic subunit ESA1 Neighboring gene Env9p Neighboring gene Srl1p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001147.6

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Role of cysteine residues in thiol modification of acyl-CoA:diacylglycerol acyltransferase 2 from yeast. Liu Q, et al. Biochemistry, 2010 Apr 20. PMID 20225889
    2. Enhanced production of fatty alcohols by engineering the TAGs synthesis pathway in Saccharomyces cerevisiae. Tang X, et al. Biotechnol Bioeng, 2015 Feb. PMID 25116045
    3. Engineering Saccharomyces cerevisiae for high yield production of α-amyrin via synergistic remodeling of α-amyrin synthase and expanding the storage pool. Yu Y, et al. Metab Eng, 2020 Nov. PMID 32841679
    4. Cloning and characterization of an acyl-CoA-dependent diacylglycerol acyltransferase 1 (DGAT1) gene from Tropaeolum majus, and a study of the functional motifs of the DGAT protein using site-directed mutagenesis to modify enzyme activity and oil content. Xu J, et al. Plant Biotechnol J, 2008 Oct. PMID 18631243
    5. Improved squalene production through increasing lipid contents in Saccharomyces cerevisiae. Wei LJ, et al. Biotechnol Bioeng, 2018 Jul. PMID 29573412

    See all (116) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Saccharomyces cerevisiae Delta9-desaturase Ole1 forms a supercomplex with Slc1 and Dga1.
      Title: Saccharomyces cerevisiae Δ9-desaturase Ole1 forms a supercomplex with Slc1 and Dga1.
    2. Triglyceride deficiency and diacylglycerol kinase1 activity lead to the upregulation of mevalonate pathway in yeast: A study for the development of potential yeast platform for improved production of triterpenoid.
      Title: Triglyceride deficiency and diacylglycerol kinase1 activity lead to the upregulation of mevalonate pathway in yeast: A study for the development of potential yeast platform for improved production of triterpenoid.
    3. Dga1 deficiency triggers fatty acid-mediated cell death.
      Title: Sterol and diacylglycerol acyltransferase deficiency triggers fatty acid-mediated cell death.
    4. Diacylglycerol acyltransferase activity was significantly increased in the Deltasnf2 disruptant strain overexpressing DGA1 as compared with the wild-type strain overexpressing DGA1
      Title: DGA1 (diacylglycerol acyltransferase gene) overexpression and leucine biosynthesis significantly increase lipid accumulation in the Deltasnf2 disruptant of Saccharomyces cerevisiae.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables 2-acylglycerol O-acyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables O-acyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables acyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables diacylglycerol O-acyltransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables diacylglycerol O-acyltransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables diacylglycerol O-acyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables diacylglycerol O-acyltransferase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in ceramide metabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in ceramide metabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in glycerol metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in intracellular triglyceride homeostasis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in lipid metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in lipid storage IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in triglyceride biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in triglyceride biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in triglyceride biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in triglyceride biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in endoplasmic reticulum HDA PubMed 
    located_in endoplasmic reticulum IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in endoplasmic reticulum membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in endoplasmic reticulum membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in lipid droplet IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in lipid droplet IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in membrane ISM
    Inferred from Sequence Model
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    diacylglycerol O-acyltransferase
    NP_014888.1
    • Diacylglycerol acyltransferase; catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor; Lro1p and Dga1p can O-acylate ceramides; localized to lipid particles

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001147.6 Reference assembly

      Range
      794076..795332 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001183664.1 → NP_014888.1  TPA: diacylglycerol O-acyltransferase [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_014888.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6W2U7, Q08650
      UniProtKB/TrEMBL
      A6ZP84, B3LJR4, C8ZGZ7, N1NVW7
      Conserved Domains (1) summary
      cl17185
      Location:80 → 410
      LPLAT; Lysophospholipid acyltransferases (LPLATs) of glycerophospholipid biosynthesis
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q08650.1

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