NEK2 NIMA related kinase 2 [Homo sapiens (human)] - Gene

Summary

Official Symbol: NEK2provided by HGNC
Official Full Name: NIMA related kinase 2provided by HGNC
Primary source: HGNC:HGNC:7745
See related: Ensembl:ENSG00000117650 MIM:604043; AllianceGenome:HGNC:7745
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: NLK1; RP67; NEK2A; HsPK21; PPP1R111
Summary: This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene. [provided by RefSeq, Feb 2011]
Expression: Biased expression in testis (RPKM 28.1), bone marrow (RPKM 3.8) and 6 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 1q32.3

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	1	NC_000001.11 (211658256..211675621, complement)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	1	NC_060925.1 (210904362..210921749, complement)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	1	NC_000001.10 (211831598..211848963, complement)

Chromosome 1 - NC_000001.11 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Function of NEK2 in clear cell renal cell carcinoma and its effect on the tumor microenvironment.
Title: Function of NEK2 in clear cell renal cell carcinoma and its effect on the tumor microenvironment.
NEK2 promotes TP53 ubiquitination to enhance the proliferation and migration of TP53 wild-type glioblastoma cells.
Title: NEK2 promotes TP53 ubiquitination to enhance the proliferation and migration of TP53 wild-type glioblastoma cells.
Abnormal methylation mediated upregulation of LINC00857 boosts malignant progression of lung adenocarcinoma by modulating the miR-486-5p/NEK2 axis.
Title: Abnormal methylation mediated upregulation of LINC00857 boosts malignant progression of lung adenocarcinoma by modulating the miR-486-5p/NEK2 axis.
NEK2 promotes the development of ovarian endometriosis and impairs decidualization by phosphorylating FOXO1.
Title: NEK2 promotes the development of ovarian endometriosis and impairs decidualization by phosphorylating FOXO1.
[Expression and clinical significance of NEK2 and EMT related molecules in oral squamous cell carcinoma].
Title: [Expression and clinical significance of NEK2 and EMT related molecules in oral squamous cell carcinoma].
Nek2A prevents centrosome clustering and induces cell death in cancer cells via KIF2C interaction.
Title: Nek2A prevents centrosome clustering and induces cell death in cancer cells via KIF2C interaction.
Inhibition of the YAP-MMB interaction and targeting NEK2 as potential therapeutic strategies for YAP-driven cancers.
Title: Inhibition of the YAP-MMB interaction and targeting NEK2 as potential therapeutic strategies for YAP-driven cancers.
Molecular characteristics, oncogenic roles, and relevant immune and pharmacogenomic features of NEK2 in gastric cancer.
Title: Molecular characteristics, oncogenic roles, and relevant immune and pharmacogenomic features of NEK2 in gastric cancer.
NEK2 is associated with poor prognosis of clear cell renal cell carcinoma and promotes tumor cell growth and metastasis.
Title: NEK2 is associated with poor prognosis of clear cell renal cell carcinoma and promotes tumor cell growth and metastasis.
NEK2 inactivates the Hippo pathway to advance the proliferation of cervical cancer cells by cooperating with STRIPAK complexes.
Title: NEK2 inactivates the Hippo pathway to advance the proliferation of cervical cancer cells by cooperating with STRIPAK complexes.

Submit: New GeneRIF Correction See all GeneRIFs (128)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Associated conditions

Description	Tests
Retinitis pigmentosa 67 MedGen: C3809954 OMIM: 615565 GeneReviews: Not available	Compare labs

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Protein interactions

Protein	Gene	Interaction	Pubs
Pr55(Gag)	gag	The amplified luminescent proximity homogeneous assay (AlphaScreen) identifies the interaction of HIV-1 Gag with NEK2	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

SHGC-76300 (e-PCR)
- UniSTS:41245

D8S2279 (e-PCR)
- UniSTS:473907

RH92597 (e-PCR)
- UniSTS:84313

D1S1423 (e-PCR)
- UniSTS:149619

D14S1215 (e-PCR)
- UniSTS:74372

SHGC-67307 (e-PCR)
- UniSTS:89991

NEK2__6227 (e-PCR)
- UniSTS:464083

RH68293 (e-PCR)
- UniSTS:76502

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables histone H2AS1 kinase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein phosphatase binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
involved_in blastocyst development	IEA Inferred from Electronic Annotation more info
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in centrosome separation	IDA Inferred from Direct Assay more info	PubMed
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in chromosome segregation	IBA Inferred from Biological aspect of Ancestor more info
involved_in chromosome segregation	IDA Inferred from Direct Assay more info	PubMed
involved_in meiotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in mitotic cell cycle	TAS Traceable Author Statement more info	PubMed
involved_in mitotic spindle assembly	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of centriole-centriole cohesion	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of telomere maintenance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein autophosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein autophosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of attachment of spindle microtubules to kinetochore	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of mitotic centrosome separation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of mitotic centrosome separation	TAS Traceable Author Statement more info	PubMed
involved_in regulation of mitotic nuclear division	TAS Traceable Author Statement more info	PubMed
involved_in spindle assembly	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
is_active_in centrosome	IBA Inferred from Biological aspect of Ancestor more info
located_in centrosome	IDA Inferred from Direct Assay more info	PubMed
located_in condensed nuclear chromosome	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	TAS Traceable Author Statement more info
colocalizes_with kinetochore	IDA Inferred from Direct Assay more info	PubMed
located_in kinetochore	IDA Inferred from Direct Assay more info	PubMed
located_in microtubule	IEA Inferred from Electronic Annotation more info
located_in midbody	IEA Inferred from Electronic Annotation more info
located_in nucleolus	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	IDA Inferred from Direct Assay more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
part_of protein-containing complex	IMP Inferred from Mutant Phenotype more info	PubMed
located_in spindle pole	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: serine/threonine-protein kinase Nek2

Names: NIMA (never in mitosis gene a)-related kinase 2; nimA-like protein kinase 1; nimA-related protein kinase 2; protein phosphatase 1, regulatory subunit 111

NP_001191111.1

EC 2.7.11.1

NP_001191112.1

EC 2.7.11.1

NP_002488.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

NG_029112.1 RefSeqGene

Range

5010..17859

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001204182.2 → NP_001191111.1 serine/threonine-protein kinase Nek2 isoform 3

Status: REVIEWED

Description

Transcript Variant: This variant (3) contains an alternate 3' terminal exon compared to variant 1. This results in a frame-shift and a shorter isoform (3) with a distinct C-terminus compared to isoform 1.

Source sequence(s)

AK225522, BT019729, DC407297

Consensus CDS

CCDS73024.1

UniProtKB/TrEMBL

B4DZU0, F6U4U2

Related

ENSP00000440237.2, ENST00000540251.5

Conserved Domains (2) summary

smart00220
Location:8 → 271

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd08217
Location:7 → 271

STKc_Nek2; Catalytic domain of the Serine/Threonine Kinase, Never In Mitosis gene A (NIMA)-related kinase 2
NM_001204183.2 → NP_001191112.1 serine/threonine-protein kinase Nek2 isoform 2

See identical proteins and their annotated locations for NP_001191112.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) differs at the 3' end compared to variant 1. This results in a frame-shift and a shorter isoform (2, also known as NEK2B) with a distinct C-terminus compared to isoform 1. Isoforms 1 and 2 have been reported to exhibit distinct pattern of expression during mitosis (PMID:11742531).

Source sequence(s)

AC096637, AY045701, BT019729, BU517064, DC407297

Consensus CDS

CCDS55682.1

UniProtKB/TrEMBL

B4DZU0

Related

ENSP00000355965.3, ENST00000366998.4

Conserved Domains (2) summary

smart00220
Location:8 → 271

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd08217
Location:7 → 271

STKc_Nek2; Catalytic domain of the Serine/Threonine Kinase, Never In Mitosis gene A (NIMA)-related kinase 2
NM_002497.4 → NP_002488.1 serine/threonine-protein kinase Nek2 isoform 1

See identical proteins and their annotated locations for NP_002488.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longest isoform (1, also known as NEK2A). The C-terminus (aa 399-445) of this isoform was shown to be responsible for its nucleolar localization (PMID:15161910). Isoforms 1 and 2 (which differ at the C-terminus) have also been reported to exhibit distinct pattern of expression during mitosis (PMID:11742531).

Source sequence(s)

AK223353, BC043502, BT019729, DC407297

Consensus CDS

CCDS1500.1

UniProtKB/Swiss-Prot

P51955, Q53FD6, Q5I1Z9, Q5VXZ1, Q6NZX8, Q7Z634, Q86XH2, Q96QN9

UniProtKB/TrEMBL

B4DZU0

Related

ENSP00000355966.4, ENST00000366999.9

Conserved Domains (1) summary

cd08217
Location:7 → 271

STKc_Nek2; Catalytic domain of the Serine/Threonine Kinase, Never In Mitosis gene A (NIMA)-related kinase 2