MCM7 mini-chromosome maintenance complex protein 7 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: MCM7
Official Full Name: mini-chromosome maintenance complex protein 7
Primary source: SGD:S000000406
Locus tag: YBR202W
See related: AllianceGenome:SGD:S000000406; FungiDB:YBR202W; VEuPathDB:YBR202W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CDC47
Summary: Enables several functions, including ATP binding activity; DNA replication origin binding activity; and MCM complex binding activity. Contributes to 3'-5' DNA helicase activity; four-way junction helicase activity; and single-stranded DNA binding activity. Involved in DNA metabolic process and constitutive heterochromatin formation. Located in cytoplasm and nucleus. Part of MCM complex; MCM core complex; and nuclear lumen. Human ortholog(s) of this gene implicated in hepatocellular carcinoma. Orthologous to human MCM7 (minichromosome maintenance complex component 7). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See MCM7 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 1

Sequence:: Chromosome: II; NC_001134.8 (625772..628309)

Chromosome II - NC_001134.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

An intact Mcm10 coiled-coil interaction surface is important for origin melting, helicase assembly and the recruitment of Pol-alpha to Mcm2-7.
Title: An intact Mcm10 coiled-coil interaction surface is important for origin melting, helicase assembly and the recruitment of Pol-α to Mcm2-7.
The tethering of SCF(Dia2) to the replisome progression complex increases the efficiency of ubiquitylation of the Mcm7 subunit of CMG. Correspondingly, loss of tethering is synthetic lethal in combination with a disassembly-defective allele of CDC48.
Title: Tethering of SCF(Dia2) to the Replisome Promotes Efficient Ubiquitylation and Disassembly of the CMG Helicase.
The data demonstrate that ORC/Cdc6 facilitates directly MCM2-7 dimerization and shows that productive MCM2-7 dimerization is essential for prereplicative complex formation.
Title: The ORC/Cdc6/MCM2-7 complex facilitates MCM2-7 dimerization during prereplicative complex formation.
origin ssDNA may stimulate the formation of the CMG complex by alleviating inhibitory interactions between Sld2 with Mcm2-7.
Title: Enabling association of the GINS protein tetramer with the mini chromosome maintenance (Mcm)2-7 protein complex by phosphorylated Sld2 protein and single-stranded origin DNA.
origin single-stranded DNA releases Sld3 from Mcm2-7, allowing GINS to bind Mcm2-7.
Title: Origin single-stranded DNA releases Sld3 protein from the Mcm2-7 complex, allowing the GINS tetramer to bind the Mcm2-7 complex.
The minimal functional unit of Mcm4/6/7 is a hexamer
Title: The effects of oligomerization on Saccharomyces cerevisiae Mcm4/6/7 function.
Analsis of the contribution of two structural motifs, the Walker B box and arginine finger, make to each Mcm2-7 ATPase active site.
Title: The Saccharomyces cerevisiae Mcm6/2 and Mcm5/3 ATPase active sites contribute to the function of the putative Mcm2-7 'gate'.
DNA runs through a central channel in the double hexamer, and, once loaded, Mcm2-7 can slide passively along double-stranded DNA.
Title: Concerted loading of Mcm2-7 double hexamers around DNA during DNA replication origin licensing.
Data indicate that a complex of the GINS and Ctf4 components of the replisome progression complex is crucial to couple MCM2-7 to DNA polymerase alpha.
Title: A key role for Ctf4 in coupling the MCM2-7 helicase to DNA polymerase alpha within the eukaryotic replisome.
incorporation of Mcm2-7 into the prereplicative complex increased the level and changes the specificity of Dbf4-dependent kinase phosphorylation of this complex
Title: Incorporation into the prereplicative complex activates the Mcm2-7 helicase for Cdc7-Dbf4 phosphorylation.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
contributes_to 3'-5' DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
contributes_to DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables DNA replication origin binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA replication origin binding	IEA Inferred from Electronic Annotation more info
enables MCM complex binding	IDA Inferred from Direct Assay more info	PubMed
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to four-way junction helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
contributes_to single-stranded DNA binding	IMP Inferred from Mutant Phenotype more info	PubMed
contributes_to single-stranded DNA helicase activity	IBA Inferred from Biological aspect of Ancestor more info
contributes_to single-stranded DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables single-stranded DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA duplex unwinding	IEA Inferred from Electronic Annotation more info
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA replication initiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA replication initiation	IEA Inferred from Electronic Annotation more info
involved_in DNA replication initiation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in DNA replication initiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA strand elongation involved in DNA replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA strand elongation involved in DNA replication	IGI Inferred from Genetic Interaction more info	PubMed
involved_in DNA strand elongation involved in DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA unwinding involved in DNA replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA unwinding involved in DNA replication	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA unwinding involved in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair via break-induced replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in double-strand break repair via break-induced replication	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair via break-induced replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nuclear DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pre-replicative complex assembly involved in nuclear cell cycle DNA replication	IDA Inferred from Direct Assay more info	PubMed
involved_in pre-replicative complex assembly involved in nuclear cell cycle DNA replication	IEA Inferred from Electronic Annotation more info
involved_in premeiotic DNA replication	IEA Inferred from Electronic Annotation more info
involved_in silent mating-type cassette heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in subtelomeric heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of CMG complex	IDA Inferred from Direct Assay more info	PubMed
part_of CMG complex	IEA Inferred from Electronic Annotation more info
part_of DNA replication preinitiation complex	IDA Inferred from Direct Assay more info	PubMed
part_of MCM complex	IBA Inferred from Biological aspect of Ancestor more info
part_of MCM complex	IDA Inferred from Direct Assay more info	PubMed
part_of MCM complex	IEA Inferred from Electronic Annotation more info
part_of MCM core complex	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome, telomeric region	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
part_of nuclear pre-replicative complex	IDA Inferred from Direct Assay more info	PubMed
part_of nuclear pre-replicative complex	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of replication fork protection complex	IDA Inferred from Direct Assay more info	PubMed
part_of replication fork protection complex	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: mini-chromosome maintenance complex protein 7

NP_009761.4

EC 3.6.4.12

Component of the Mcm2-7 hexameric helicase complex; MCM2-7 primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001134.8 Reference assembly

Range

625772..628309

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178550.4 → NP_009761.4 TPA: mini-chromosome maintenance complex protein 7 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009761.4

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VQK0, P38132

UniProtKB/TrEMBL

A6ZLE4, B3LMV6, C7GKG5, G2W9K7, N1P8F4

Conserved Domains (2) summary

smart00350
Location:223 → 724

MCM; minichromosome maintenance proteins

pfam14551
Location:52 → 123

MCM_N; MCM N-terminal domain