MAF1 RNA polymerase III-inhibiting protein MAF1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: MAF1
Gene description: RNA polymerase III-inhibiting protein MAF1
Primary source: FungiDB:YDR005C
Locus tag: YDR005C
See related: SGD:S000002412; AllianceGenome:SGD:S000002412; VEuPathDB:YDR005C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables RNA polymerase III core binding activity. Involved in negative regulation of transcription by RNA polymerase III and transfer RNA gene-mediated silencing. Located in cytoplasm and nucleolus. Orthologous to human MAF1 (MAF1 homolog, negative regulator of RNA polymerase III). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See MAF1 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 2

Sequence:: Chromosome: IV; NC_001136.10 (456836..458103, complement)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Structural basis for RNA polymerase III transcription repression by Maf1 in humans and yeast has been uncovered.
Title: Structural basis for RNA polymerase III transcription repression by Maf1.
In a mutant compromised in RNAP III activity, there is a repartitioning towards amino acids synthesis de novo at the expense of glycolytic throughput. Conversely, cells lacking Maf1 protein have greater potential for glycolytic flux.
Title: Glycolytic flux in Saccharomyces cerevisiae is dependent on RNA polymerase III and its negative regulator Maf1.
correlation between CCA synthesis and Pol III regulation by Maf1 could be important in coordination of tRNA transcription, processing and regulation of translation
Title: Maf1-mediated regulation of yeast RNA polymerase III is correlated with CCA addition at the 3' end of tRNA precursors.
Sub1 and Maf1 also exhibit an opposite role on yeast chronological life span
Title: Sub1 and Maf1, two effectors of RNA polymerase III, are involved in the yeast quiescence cycle.
PKA modulates Maf1 protein abundance for stress and lifespan but not growth
Title: Distinct regulation of Maf1 for lifespan extension by Protein kinase A and Sch9.
Hypothesized that decreased FBP1 mRNA level could be due to the effect of Maf1 on tgm silencing (tRNA gene mediated silencing).
Title: Maf1, repressor of tRNA transcription, is involved in the control of gluconeogenetic genes in Saccharomyces cerevisiae.
Maf1-mediated repression of RNA polymerase III transcription inhibits tRNA degradation via RTD pathway.
Title: Maf1-mediated repression of RNA polymerase III transcription inhibits tRNA degradation via RTD pathway.
Recovery of RNA polymerase III transcription from the glycerol-repressed state: revisiting the role of protein kinase CK2 in Maf1 phosphoregulation.
Title: Recovery of RNA polymerase III transcription from the glycerol-repressed state: revisiting the role of protein kinase CK2 in Maf1 phosphoregulation.
Maf1-mediated transcription control and nuclear export by Los1 are two major stages of tRNA biosynthesis that are regulated by environmental conditions in a coordinated manner.
Title: Maf1 protein, repressor of RNA polymerase III, indirectly affects tRNA processing.
an important role of CK2-mediated Maf1 phosphorylation in triggering Pol III activation.
Title: Casein kinase II-mediated phosphorylation of general repressor Maf1 triggers RNA polymerase III activation.

Submit: New GeneRIF Correction See all GeneRIFs (20)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables RNA polymerase III core binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase III core binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in negative regulation of transcription by RNA polymerase III	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of transcription by RNA polymerase III	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of transcription by RNA polymerase III	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of transcription by RNA polymerase III	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of transcription by RNA polymerase III	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase III	IPI Inferred from Physical Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleolus	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: RNA polymerase III-inhibiting protein MAF1

NP_010288.1

Highly conserved negative regulator of RNA polymerase III; involved in tRNA processing and stability; inhibits tRNA degradation via rapid tRNA decay (RTD) pathway; binds N-terminal domain of Rpc160p subunit of Pol III to prevent closed-complex formation; regulated by phosphorylation mediated by TORC1, protein kinase A, Sch9p, casein kinase 2; localizes to cytoplasm during vegetative growth and translocates to nucleus and nucleolus under stress conditions

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

456836..458103 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180313.1 → NP_010288.1 TPA: RNA polymerase III-inhibiting protein MAF1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010288.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VRZ3, P41910

UniProtKB/TrEMBL

A6ZXX1, B3LGQ3, C8Z4S5, G2WCF9, N1P6A6

Conserved Domains (1) summary

cl02103
Location:81 → 387

Maf1; Maf1 regulator