SEC24 COPII subunit SEC24 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SEC24
Official Full Name: COPII subunit SEC24
Primary source: SGD:S000001371
Locus tag: YIL109C
See related: AllianceGenome:SGD:S000001371; FungiDB:YIL109C; VEuPathDB:YIL109C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: ANU1
Summary: Enables signal sequence binding activity. Involved in COPII-coated vesicle cargo loading and macroautophagy. Located in fungal-type vacuole membrane. Part of COPII vesicle coat. Orthologous to human SEC24A (SEC24 homolog A, COPII coat complex component) and SEC24B (SEC24 homolog B, COPII coat complex component). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See SEC24 in Genome Data Viewer

Location:: chromosome: IX

Exon count:: 1

Sequence:: Chromosome: IX; NC_001141.2 (157385..160165, complement)

Chromosome IX - NC_001141.2 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001141.2

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

We identified two amino acid polymorphisms in SEC24 that cause strong sensitivity to ethanol at high temperature and more limited sensitivity to temperature in the absence of ethanol.
Title: Genetic Basis of Variation in Heat and Ethanol Tolerance in Saccharomyces cerevisiae.
Here the authors show that phosphorylation of conserved amino acids on the membrane-distal surface of the Saccharomyces cerevisiae COPII cargo adaptor, Sec24, reprograms COPII vesicles for autophagy.
Title: Sec24 phosphorylation regulates autophagosome abundance during nutrient deprivation.
Erv14 interaction with a novel cargo-binding surface on Sec24 is needed for efficient trafficking of all its clients. Some clients also engage an adjacent cargo-binding domain of Sec24, suggesting a novel mode of dual interaction between cargo and coat.
Title: Sec24 is a coincidence detector that simultaneously binds two signals to drive ER export.
Distribution of Sec24 isoforms to each ER exit site is dynamically regulated in Saccharomyces cerevisiae.
Title: Distribution of Sec24 isoforms to each ER exit site is dynamically regulated in Saccharomyces cerevisiae.
the cytoplasmic N-terminal domain of Chs2p, which contains the CDK1 phosphorylation sites, interacts with the COPII component Sec24p in a yeast two-hybrid assay and that phosphomimetic substitutions of serines at the CDK1 consensus sites reduces the interaction.
Title: Phosphorylation of Chs2p regulates interaction with COPII.
association of Sec24p with Sec16p creates a regulatory complex that retards the GTPase activity of the COPII coat to prevent premature vesicle scission
Title: Sec24p and Sec16p cooperate to regulate the GTP cycle of the COPII coat.
Results describe a novel set of mutations in Sec24p that distinguish two adjacent cargo-binding sites and examine the ability of Sec22p to rescue these mutations.
Title: Genetic analysis of yeast Sec24p mutants suggests cargo binding is not co-operative during ER export.
only Sed5p was depleted in vesicles made with Sec24p A-site mutants
Title: ER-Golgi transport defects are associated with mutations in the Sed5p-binding domain of the COPII coat subunit, Sec24p.
a soluble cargo protein, Hsp150, is selected actively and specifically to budding sites lacking normal Sec24p by a signature residing in its C-terminal domain
Title: Active and specific recruitment of a soluble cargo protein for endoplasmic reticulum exit in the absence of functional COPII component Sec24p.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables SNARE binding	IBA Inferred from Biological aspect of Ancestor more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables signal sequence binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables zinc ion binding	IBA Inferred from Biological aspect of Ancestor more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in COPII-coated vesicle cargo loading	IBA Inferred from Biological aspect of Ancestor more info
involved_in COPII-coated vesicle cargo loading	IDA Inferred from Direct Assay more info	PubMed
involved_in endoplasmic reticulum to Golgi vesicle-mediated transport	IEA Inferred from Electronic Annotation more info
involved_in intracellular protein transport	IEA Inferred from Electronic Annotation more info
involved_in macroautophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein transport	IEA Inferred from Electronic Annotation more info
involved_in vesicle-mediated transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of COPII vesicle coat	IBA Inferred from Biological aspect of Ancestor more info
part_of COPII vesicle coat	IDA Inferred from Direct Assay more info	PubMed
part_of COPII vesicle coat	IEA Inferred from Electronic Annotation more info
located_in ER to Golgi transport vesicle membrane	IEA Inferred from Electronic Annotation more info
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
located_in Golgi membrane	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic vesicle	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
is_active_in endoplasmic reticulum exit site	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
located_in fungal-type vacuole membrane	HDA more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: COPII subunit SEC24

NP_012157.3

Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat; required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb3p; SEC24 has a paralog, SFB2, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001141.2 Reference assembly

Range

157385..160165 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179457.3 → NP_012157.3 TPA: COPII subunit SEC24 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012157.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VVH8, P40482

UniProtKB/TrEMBL

A6ZVG8, B3LTV6, C7GKN5, N1P9B0

Conserved Domains (1) summary

COG5028
Location:1 → 926

COG5028; Vesicle coat complex COPII, subunit SEC24/subunit SFB2/subunit SFB3 [Intracellular trafficking and secretion]