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    ENT3 Ent3p [ Saccharomyces cerevisiae S288C ]

    Gene ID: 853589, updated on 2-Nov-2024

    Summary

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    Official Symbol
    ENT3
    Official Full Name
    Ent3p
    Primary source
    SGD:S000003886
    Locus tag
    YJR125C
    See related
    AllianceGenome:SGD:S000003886; FungiDB:YJR125C; VEuPathDB:YJR125C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables clathrin binding activity; phosphatidylinositol-3,5-bisphosphate binding activity; and phosphatidylinositol-3-phosphate binding activity. Involved in actin cytoskeleton organization; cytosolic transport; and late endosome to vacuole transport via multivesicular body sorting pathway. Located in endosome. Part of clathrin vesicle coat. Orthologous to several human genes including CLINT1 (clathrin interactor 1). [provided by Alliance of Genome Resources, Nov 2024]
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    Genomic context

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    See ENT3 in Genome Data Viewer
    Location:
    chromosome: X
    Exon count:
    1
    Sequence:
    Chromosome: X; NC_001142.9 (654737..655963, complement)

    Chromosome X - NC_001142.9Genomic Context describing neighboring genes Neighboring gene ribosomal 40S subunit protein S5 Neighboring gene uncharacterized protein Neighboring gene putative zinc metalloprotease Neighboring gene Rsf2p Neighboring gene uncharacterized protein

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001142.9

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Ent3p Is a PtdIns(3,5)P2 effector required for protein sorting to the multivesicular body. Friant S, et al. Dev Cell, 2003 Sep. PMID 12967568
    2. Epsin N-terminal homology domains bind on opposite sides of two SNAREs. Wang J, et al. Proc Natl Acad Sci U S A, 2011 Jul 26. PMID 21746902, Free PMC Article
    3. Distinct functional domains of the epsin-related Ent5p, a cargo adaptor for the SNARE Tlg2p in transport between endosomes and Golgi. Petersen L, et al. Traffic, 2023 Oct. PMID 37434343
    4. Ent5p is required with Ent3p and Vps27p for ubiquitin-dependent protein sorting into the multivesicular body. Eugster A, et al. Mol Biol Cell, 2004 Jul. PMID 15107463, Free PMC Article
    5. Structural basis for the specificity of the GAE domain of yGGA2 for its accessory proteins Ent3 and Ent5 . Fang P, et al. Biochemistry, 2010 Sep 14. PMID 20704189

    See all (57) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Here we show not all acidic-phenylalanine motifs in Ent3 are active for yGGA2_GAE domain binding
      Title: Structural basis for the specificity of the GAE domain of yGGA2 for its accessory proteins Ent3 and Ent5 .
    2. Ent3p is a connector between PtdIns(3,5)P(2)- and the Vps27p-ubiquitin-driven sorting machinery at the multivesicular body.
      Title: Ent5p is required with Ent3p and Vps27p for ubiquitin-dependent protein sorting into the multivesicular body.
    3. the clathrin adaptors AP-1, Ent3p, Ent5p, and the Ggas cooperate in different ways to sort proteins between the TGN and the endosomes
      Title: Ent3p and Ent5p exhibit cargo-specific functions in trafficking proteins between the trans-Golgi network and the endosomes in yeast.
    4. These results differentiate Ent3p and Ent5p function in vivo, suggesting that Ent3p acts primarily with Gga proteins, whereas Ent5p acts with both AP-1 and Gga proteins but is more critical for AP-1-mediated transport.
      Title: Distinct roles for TGN/endosome epsin-like adaptors Ent3p and Ent5p.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables clathrin binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables clathrin binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables lipid binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables phosphatidylinositol-3,5-bisphosphate binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables phosphatidylinositol-3-phosphate binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables phospholipid binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in Golgi to endosome transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in Golgi to endosome transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in actin cytoskeleton organization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in early endosome to Golgi transport IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in endocytosis IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in late endosome to vacuole transport via multivesicular body sorting pathway IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in multivesicular body sorting pathway IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in Golgi apparatus IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of clathrin vesicle coat IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of clathrin vesicle coat IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in clathrin-coated vesicle membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytoplasmic vesicle IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in endosome IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in endosome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  

    General protein information

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    Preferred Names
    Ent3p
    NP_012659.1
    • Protein containing an N-terminal epsin-like domain; involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001142.9 Reference assembly

      Range
      654737..655963 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001181783.1 → NP_012659.1  TPA: Ent3p [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_012659.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VWU4, P47160
      UniProtKB/TrEMBL
      A6ZQ81, B3LQK9, B5VLR7, C7GM87, C8ZBS3, G2WHC8, N1P1S3
      Conserved Domains (1) summary
      cd16992
      Location:32 → 152
      ENTH_Ent3; Epsin N-Terminal Homology (ENTH) domain of Yeast Ent3 and similar proteins
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P47160.1

    Gene LinkOut

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