RAD5 DNA helicase RAD5 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RAD5
Official Full Name: DNA helicase RAD5
Primary source: SGD:S000004022
Locus tag: YLR032W
See related: AllianceGenome:SGD:S000004022; FungiDB:YLR032W; VEuPathDB:YLR032W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: REV2; SNM2
Summary: Enables Y-form DNA binding activity; four-way junction DNA binding activity; and four-way junction helicase activity. Involved in DNA repair; free ubiquitin chain polymerization; and protein polyubiquitination. Located in chromatin and chromosome, telomeric region. Used to study gastrointestinal system cancer. Orthologous to human HLTF (helicase like transcription factor). [provided by Alliance of Genome Resources, Oct 2024]
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Genomic context

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See RAD5 in Genome Data Viewer

Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (204991..208500)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Spatial regulation of DNA damage tolerance protein Rad5 interconnects genome stability maintenance and proteostasis networks.
Title: Spatial regulation of DNA damage tolerance protein Rad5 interconnects genome stability maintenance and proteostasis networks.
Helicase activities of Rad5 and Rrm3 genetically interact in the prevention of recombinogenic DNA lesions in Saccharomyces cerevisiae.
Title: Helicase activities of Rad5 and Rrm3 genetically interact in the prevention of recombinogenic DNA lesions in Saccharomyces cerevisiae.
Fork-Remodeling Helicase Rad5 Preferentially Reverses Replication Forks with Gaps in the Leading Strand.
Title: Fork-Remodeling Helicase Rad5 Preferentially Reverses Replication Forks with Gaps in the Leading Strand.
The Rad5 Helicase and RING Domains Contribute to Genome Stability through their Independent Catalytic Activities.
Title: The Rad5 Helicase and RING Domains Contribute to Genome Stability through their Independent Catalytic Activities.
Contractions of the C-Terminal Domain of Saccharomyces cerevisiae Rpb1p Are Mediated by Rad5p.
Title: Contractions of the C-Terminal Domain of Saccharomyces cerevisiae Rpb1p Are Mediated by Rad5p.
The Zn-finger of Saccharomyces cerevisiae Rad18 and its adjacent region mediate interaction with Rad5.
Title: The Zn-finger of Saccharomyces cerevisiae Rad18 and its adjacent region mediate interaction with Rad5.
Prevention of unwanted recombination at damaged replication forks.
Title: Prevention of unwanted recombination at damaged replication forks.
MutSalpha deficiency increases tolerance to DNA damage in yeast lacking postreplication repair.
Title: MutSα deficiency increases tolerance to DNA damage in yeast lacking postreplication repair.
The direct effect of Rad5 on replication forks in vivo, increased recombination, and cisplatin sensitivity predicts similar consequences for dysregulated HLTF in cancer.
Title: Rad5 dysregulation drives hyperactive recombination at replication forks resulting in cisplatin sensitivity and genome instability.
Rad5 binds to the sites of stressed DNA replication forks, where it recruits TLS polymerase, DNA polymerase zeta, to repair single-stranded DNA (ssDNA) gaps, preventing mitotic defects and chromosome breaks.
Title: Rad5 Recruits Error-Prone DNA Polymerases for Mutagenic Repair of ssDNA Gaps on Undamaged Templates.

Submit: New GeneRIF Correction See all GeneRIFs (29)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP-dependent activity, acting on DNA	IBA Inferred from Biological aspect of Ancestor more info
enables ATP-dependent activity, acting on DNA	IDA Inferred from Direct Assay more info	PubMed
enables ATP-dependent chromatin remodeler activity	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables Y-form DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables four-way junction DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables four-way junction helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA duplex unwinding	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair	IGI Inferred from Genetic Interaction more info	PubMed
involved_in double-strand break repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in error-free postreplication DNA repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in error-free translesion synthesis	IDA Inferred from Direct Assay more info	PubMed
involved_in error-free translesion synthesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in error-prone translesion synthesis	IDA Inferred from Direct Assay more info	PubMed
involved_in error-prone translesion synthesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in free ubiquitin chain polymerization	IDA Inferred from Direct Assay more info	PubMed
involved_in postreplication repair	IDA Inferred from Direct Assay more info	PubMed
involved_in protein polyubiquitination	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in chromatin	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome, telomeric region	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: DNA helicase RAD5

NP_013132.1

DNA helicase/Ubiquitin ligase; involved in error-free DNA damage tolerance (DDT), replication fork regression during postreplication repair by template switching, and error-prone translesion synthesis; required for UV and MMS-associated unequal sister chromatid exchange (SCE) but not for double-strand break associated unequal SCE; promotes synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; human homolog HLTF can complement yeast null mutant

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001144.5 Reference assembly

Range

204991..208500

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181919.1 → NP_013132.1 TPA: DNA helicase RAD5 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013132.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VY34, P32849

UniProtKB/TrEMBL

B3LT04

Conserved Domains (2) summary

COG0553
Location:116 → 1169

HepA; Superfamily II DNA or RNA helicase, SNF2 family [Transcription, Replication, recombination and repair]

pfam00097
Location:914 → 960

zf-C3HC4; Zinc finger, C3HC4 type (RING finger)