TOP3 DNA topoisomerase 3 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: TOP3
Official Full Name: DNA topoisomerase 3
Primary source: SGD:S000004224
Locus tag: YLR234W
See related: AllianceGenome:SGD:S000004224; FungiDB:YLR234W; VEuPathDB:YLR234W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: EDR1
Summary: Enables DNA topoisomerase type I (single strand cut, ATP-independent) activity. Involved in chromosome organization; reciprocal meiotic recombination; and regulation of DNA recombination. Part of RecQ family helicase-topoisomerase III complex. Human ortholog(s) of this gene implicated in autosomal recessive progressive external ophthalmoplegia with mitochondrial DNA deletions 5. Orthologous to human TOP3A (DNA topoisomerase III alpha). [provided by Alliance of Genome Resources, Oct 2024]
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Genomic context

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See TOP3 in Genome Data Viewer

Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (609783..611753)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Deletion of ULS1 confers damage tolerance in sgs1 mutants through a Top3-dependent D-loop mediated fork restart pathway
Title: Deletion of ULS1 confers damage tolerance in sgs1 mutants through a Top3-dependent D-loop mediated fork restart pathway.
Although Top3 and Sgs1 interact without sumoylation, sumoylated forms of Sgs1, particularly those containing K621 conjugates, are preferentially enriched in complexes with Top3.
Title: Smc5/6 Mediated Sumoylation of the Sgs1-Top3-Rmi1 Complex Promotes Removal of Recombination Intermediates.
Our data are consistent with a redundant role of Top2 and Sgs1 together with Top3 (Sgs1-Top3) in replication fork merging at rDNA barriers.
Title: Top2 and Sgs1-Top3 Act Redundantly to Ensure rDNA Replication Termination.
The strand-passage activity of Top3-Rmi1 is required for all known functions of Sgs1 in meiotic recombination, including channeling JMs into physiological crossover and noncrossover pathways, and suppression of non-allelic recombination.
Title: Pervasive and essential roles of the Top3-Rmi1 decatenase orchestrate recombination and facilitate chromosome segregation in meiosis.
Top3 specifically disrupts D loops mediated by yeast Rad51/Rad54.
Title: Top3-Rmi1 dissolve Rad51-mediated D loops by a topoisomerase-based mechanism.
Top3-Rmi1 acts in all meiotic recombination functions previously associated with Sgs1, most notably as an early recombination intermediate chaperone, promoting regulated crossover and noncrossover recombination.
Title: Top3-Rmi1 DNA single-strand decatenase is integral to the formation and resolution of meiotic recombination intermediates.
the roles played by Top3 and the STR complex as a whole during the resolution of replication-associated recombination intermediates.
Title: Resolution by unassisted Top3 points to template switch recombination intermediates during DNA replication.
Rmi1 has a unique regulatory capacity that slows DNA relaxation by Top3 but stimulates DNA decatenation.
Title: Decatenation of DNA by the S. cerevisiae Sgs1-Top3-Rmi1 and RPA complex: a mechanism for disentangling chromosomes.
HJ resolvases and Sgs1-Top3-Rmi1 comprise two independent processes to deal with HJ-containing DNA intermediates arising during HRR in S-phase.
Title: Holliday junction-containing DNA structures persist in cells lacking Sgs1 or Top3 following exposure to DNA damage.
Study established that Dna2, Sgs1 and RPA constitute a minimal protein complex capable of DNA resection in vitro; in addition it was found that both the Top3 and Rmi1 complex and the Mre11-Rad50-Xrs2 complex have important roles as stimulatory components.
Title: DNA end resection by Dna2-Sgs1-RPA and its stimulation by Top3-Rmi1 and Mre11-Rad50-Xrs2.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA topoisomerase activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA topoisomerase activity	IEA Inferred from Electronic Annotation more info
enables DNA topoisomerase type I (single strand cut, ATP-independent) activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA topoisomerase type I (single strand cut, ATP-independent) activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA topoisomerase type I (single strand cut, ATP-independent) activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA repair	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA topological change	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA topological change	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA topological change	IEA Inferred from Electronic Annotation more info
involved_in mitotic sister chromatid cohesion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in reciprocal meiotic recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in telomere maintenance via recombination	IGI Inferred from Genetic Interaction more info	PubMed
involved_in telomere maintenance via telomerase	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of RecQ family helicase-topoisomerase III complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RecQ family helicase-topoisomerase III complex	IDA Inferred from Direct Assay more info	PubMed
part_of RecQ family helicase-topoisomerase III complex	IPI Inferred from Physical Interaction more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: DNA topoisomerase 3

NP_013335.1

EC 5.6.2.1

DNA Topoisomerase III; conserved protein that functions in a complex with Sgs1p and Rmi1p to relax single-stranded negatively-supercoiled DNA preferentially; DNA catenation/decatenation activity is stimulated by RPA and Sgs1p-Top3p-Rmi1p; involved in telomere stability and regulation of mitotic recombination

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001144.5 Reference assembly

Range

609783..611753

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182121.1 → NP_013335.1 TPA: DNA topoisomerase 3 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013335.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VYN4, P13099

UniProtKB/TrEMBL

B3RHC1, C7GR00, C8ZDJ8, N1NZV2

Conserved Domains (1) summary

COG0550
Location:2 → 640

TopA; DNA topoisomerase IA [Replication, recombination and repair]