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    CEP3 Cep3p [ Saccharomyces cerevisiae S288C ]

    Gene ID: 855204, updated on 29-Jun-2024

    Summary

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    Gene symbol
    CEP3
    Gene description
    Cep3p
    Primary source
    FungiDB:YMR168C
    Locus tag
    YMR168C
    See related
    SGD:S000004778; AllianceGenome:SGD:S000004778; VEuPathDB:YMR168C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    CBF3; CSL1
    Summary
    Enables DNA binding activity, bending and centromeric DNA binding activity. Involved in mitotic spindle assembly checkpoint signaling and septin ring assembly. Located in kinetochore. Part of CBF3 complex. [provided by Alliance of Genome Resources, Apr 2022]
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    Genomic context

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    See CEP3 in Genome Data Viewer
    Location:
    chromosome: XIII
    Exon count:
    1
    Sequence:
    Chromosome: XIII; NC_001145.3 (597332..599158, complement)

    Chromosome XIII - NC_001145.3Genomic Context describing neighboring genes Neighboring gene Mme1p Neighboring gene mismatch repair ATPase MLH1 Neighboring gene aldehyde dehydrogenase (NAD(+)) ALD3 Neighboring gene aldehyde dehydrogenase (NAD(+)) ALD2

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001145.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Insights into kinetochore-DNA interactions from the structure of Cep3Delta. Purvis A, et al. EMBO Rep, 2008 Jan. PMID 18064045, Free PMC Article
    2. Crystal structure of the yeast inner kinetochore subunit Cep3p. Bellizzi JJ 3rd, et al. Structure, 2007 Nov. PMID 17997968, Free PMC Article
    3. Insights from modeling the 3D structure of DNA-CBF3b complex. Chou KC. J Proteome Res, 2005 Sep-Oct. PMID 16212418
    4. Probing the architecture of a simple kinetochore using DNA-protein crosslinking. Espelin CW, et al. J Cell Biol, 1997 Dec 15. PMID 9396745, Free PMC Article
    5. Factors required for the binding of reassembled yeast kinetochores to microtubules in vitro. Sorger PK, et al. J Cell Biol, 1994 Nov. PMID 7962081, Free PMC Article

    See all (87) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Data suggest that the binding sites for Cbf1 and CEP3 (CBF3 subunit) are included within the centromeric nucleosome has important implications for models of the centromeric nucleosome and for kinetochore function.
      Title: The centromeric nucleosome of budding yeast is perfectly positioned and covers the entire centromere.
    2. The crystal structure of a large section of the protein Cep3 from CBF3, which is the only component with obvious DNA-binding motifs, was determined.
      Title: Insights into kinetochore-DNA interactions from the structure of Cep3Delta.
    3. the crystal structure, determined at 2.8 A resolution by multiple isomorphous replacement with anomalous scattering, of a truncated Cep3p
      Title: Crystal structure of the yeast inner kinetochore subunit Cep3p.
    4. Data show that the amino terminus of Sgt1p interacts with CBF3 subunits Skp1p and Ctf13p.
      Title: The interaction between Sgt1p and Skp1p is regulated by HSP90 chaperones and is required for proper CBF3 assembly.
    5. Three-dimensional structural model of the DNA-CBF3b complex. [CBF3B]
      Title: Insights from modeling the 3D structure of DNA-CBF3b complex.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA binding, bending IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA-binding transcription factor activity, RNA polymerase II-specific IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables centromeric DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables zinc ion binding RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in mitotic spindle assembly checkpoint signaling IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of DNA-templated transcription IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of transcription by RNA polymerase II IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in septin ring assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of CBF3 complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in chromosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in chromosome, centromeric region IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in kinetochore IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    Cep3p
    NP_013891.1
    • Essential kinetochore protein; component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001145.3 Reference assembly

      Range
      597332..599158 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001182672.1 → NP_013891.1  TPA: Cep3p [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_013891.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VZZ0, P40969
      UniProtKB/TrEMBL
      A0A8H4BXG6, B3LM55, C7GU54, C8ZF28, N1P0F3
      Conserved Domains (2) summary
      cd12147
      Location:55 → 606
      Cep3_C; C-terminal domain of the Cep3, a subunit of the yeast centromere-binding factor 3
      pfam00172
      Location:12 → 48
      Zn_clus; Fungal Zn(2)-Cys(6) binuclear cluster domain
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P40969.1

    Gene LinkOut

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