NOB1 rRNA-binding endoribonuclease [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: NOB1
Official Full Name: rRNA-binding endoribonuclease
Primary source: SGD:S000005582
Locus tag: YOR056C
See related: AllianceGenome:SGD:S000005582; FungiDB:YOR056C; VEuPathDB:YOR056C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: YOR29-07
Summary: Enables RNA endonuclease activity and small ribosomal subunit rRNA binding activity. Involved in maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) and proteasome assembly. Located in nucleus. Part of preribosome, small subunit precursor. Used to study colorectal cancer and osteosarcoma. Orthologous to human NOB1 (NIN1 (RPN12) binding protein 1 homolog). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See NOB1 in Genome Data Viewer

Location:: chromosome: XV

Exon count:: 1

Sequence:: Chromosome: XV; NC_001147.6 (430247..431626, complement)

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data report that cleavage of pre-rRNA at the 3'-end of 18 S rRNA, requires functional interactions between Nob1 and Prp43, and the cofactor Pfa1.
Title: RNA helicase Prp43 and its co-factor Pfa1 promote 20 to 18 S rRNA processing catalyzed by the endonuclease Nob1.
Data demonstrate that Nob1's binding site centers around the 3'-end of 18S rRNA and also locate Nob1's suggested active site.
Title: Nob1 binds the single-stranded cleavage site D at the 3'-end of 18S rRNA with its PIN domain.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables RNA endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables RNA endonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables RNA endonuclease activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nuclease activity	IEA Inferred from Electronic Annotation more info
enables small ribosomal subunit rRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in maturation of SSU-rRNA	IBA Inferred from Biological aspect of Ancestor more info
involved_in maturation of SSU-rRNA	IDA Inferred from Direct Assay more info	PubMed
involved_in maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteasome assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteasome assembly	IPI Inferred from Physical Interaction more info	PubMed
involved_in ribosomal small subunit biogenesis	IEA Inferred from Electronic Annotation more info
involved_in ribosome biogenesis	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in nucleolus	IEA Inferred from Electronic Annotation more info
located_in nucleus	HDA more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of preribosome, small subunit precursor	IBA Inferred from Biological aspect of Ancestor more info
part_of preribosome, small subunit precursor	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: rRNA-binding endoribonuclease

NP_014699.1

Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6 Reference assembly

Range

430247..431626 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183475.1 → NP_014699.1 TPA: rRNA-binding endoribonuclease [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014699.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W2B9, O00021, Q08444

UniProtKB/TrEMBL

A6ZNQ8, B3LJA5, C8ZI17, G2WMT4, N1NWH4

Conserved Domains (2) summary

COG1439
Location:102 → 346

Nob1; rRNA maturation endonuclease Nob1 [Translation, ribosomal structure and biogenesis]

pfam17146
Location:12 → 102

PIN_6; PIN domain of ribonuclease