ORC4 origin recognition complex subunit 4 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: ORC4
Gene description: origin recognition complex subunit 4
Primary source: FungiDB:YPR162C
Locus tag: YPR162C
See related: SGD:S000006366; AllianceGenome:SGD:S000006366; VEuPathDB:YPR162C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables DNA replication origin binding activity. Involved in DNA replication initiation; pre-replicative complex assembly involved in nuclear cell cycle DNA replication; and silent mating-type cassette heterochromatin assembly. Part of DNA replication preinitiation complex; nuclear origin of replication recognition complex; and nuclear pre-replicative complex. Used to study Meier-Gorlin syndrome. Human ortholog(s) of this gene implicated in Meier-Gorlin syndrome 2. Orthologous to human ORC4 (origin recognition complex subunit 4). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See ORC4 in Genome Data Viewer

Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (866715..868304, complement)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Evolution of DNA replication origin specification and gene silencing mechanisms.
Title: Evolution of DNA replication origin specification and gene silencing mechanisms.
Since the site of one of the missense mutations in the human ORC4 alleles is conserved between humans and yeast, we sought to determine in what way this single amino acid change affects the process of chromosome replication, by introducing the comparable mutation into yeast (orc4Y232C).
Title: Defective replication initiation results in locus specific chromosome breakage and a ribosomal RNA deficiency in yeast.
interaction is mediated by the C-terminal region of Orc4p, and the N-terminal region of Orc5p
Title: Mechanism for the degradation of origin recognition complex containing Orc5p with a defective Walker A motif and its suppression by over-production of Orc4p in yeast cells.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA replication origin binding	IBA Inferred from Biological aspect of Ancestor more info
enables DNA replication origin binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA replication initiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA replication initiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pre-replicative complex assembly involved in nuclear cell cycle DNA replication	IDA Inferred from Direct Assay more info	PubMed
involved_in silent mating-type cassette heterochromatin formation	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of DNA replication preinitiation complex	IDA Inferred from Direct Assay more info	PubMed
part_of nuclear origin of replication recognition complex	IBA Inferred from Biological aspect of Ancestor more info
part_of nuclear origin of replication recognition complex	IDA Inferred from Direct Assay more info	PubMed
part_of nuclear origin of replication recognition complex	IMP Inferred from Mutant Phenotype more info	PubMed
part_of nuclear pre-replicative complex	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of origin recognition complex	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: origin recognition complex subunit 4

NP_015488.1

Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; ORC4 has a paralog, RIF2, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001148.4 Reference assembly

Range

866715..868304 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001184259.1 → NP_015488.1 TPA: origin recognition complex subunit 4 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_015488.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W4G3, P54791

UniProtKB/TrEMBL

A0A6A5Q2Y6, A6ZX38, B3LKC3, C7GP45, C8ZJI8, N1NWJ7

Conserved Domains (2) summary

COG1474
Location:95 → 276

CDC6; Cdc6-related protein, AAA superfamily ATPase [Replication, recombination and repair]

pfam14629
Location:286 → 517

ORC4_C; Origin recognition complex (ORC) subunit 4 C-terminus