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    DPM1 dolichyl-phosphate beta-D-mannosyltransferase [ Saccharomyces cerevisiae S288C ]

    Gene ID: 856313, updated on 28-Oct-2024

    Summary

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    Official Symbol
    DPM1
    Official Full Name
    dolichyl-phosphate beta-D-mannosyltransferase
    Primary source
    SGD:S000006387
    Locus tag
    YPR183W
    See related
    AllianceGenome:SGD:S000006387; FungiDB:YPR183W; VEuPathDB:YPR183W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    SED3
    Summary
    Enables dolichyl-phosphate beta-D-mannosyltransferase activity. Involved in GPI anchor biosynthetic process and dolichol-linked oligosaccharide biosynthetic process. Located in endoplasmic reticulum and nuclear outer membrane-endoplasmic reticulum membrane network. Used to study breast cancer. Human ortholog(s) of this gene implicated in congenital disorder of glycosylation Ie. Orthologous to human DPM1 (dolichyl-phosphate mannosyltransferase subunit 1, catalytic). [provided by Alliance of Genome Resources, Oct 2024]
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    Genomic context

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    See DPM1 in Genome Data Viewer
    Location:
    chromosome: XVI
    Exon count:
    1
    Sequence:
    Chromosome: XVI; NC_001148.4 (900755..901558)

    Chromosome XVI - NC_001148.4Genomic Context describing neighboring genes Neighboring gene GTPase-activating protein SEC23 Neighboring gene mRNA splicing protein SMX3 Neighboring gene bifunctional 4-alpha-glucanotransferase/amylo-alpha-1,6-glucosidase Neighboring gene serine/threonine protein kinase regulatory subunit ATG13

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001148.4

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The isolation of a Dol-P-Man synthase from Ustilago maydis that functions in Saccharomyces cerevisiae. Zimmerman JW, et al. Yeast, 1996 Jun 30. PMID 8813763
    2. A single point mutation resulting in an adversely reduced expression of DPM2 in the Lec15.1 cells. Pu L, et al. Biochem Biophys Res Commun, 2003 Dec 19. PMID 14680801
    3. Dolichol phosphate mannose synthase from the filamentous fungus Trichoderma reesei belongs to the human and Schizosaccharomyces pombe class of the enzyme. Kruszewska JS, et al. Glycobiology, 2000 Oct. PMID 11030744
    4. Investigations of the active site of Saccharomyces cerevisiae dolichyl-phosphate-mannose synthase using fluorescent labeled dolichyl-phosphate derivatives. Xing J, et al. Biochemistry, 2000 Jul 11. PMID 10891068
    5. Regulation of the biosynthesis of N-acetylglucosaminylpyrophosphoryldolichol, feedback and product inhibition. Kean EL, et al. J Biol Chem, 1999 Nov 26. PMID 10567375

    See all (109) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Two temperature-sensitive Saccharomyces cerevisiae mutants, sec59-1 and dpm1-6, impaired, respectively, in dolichol kinase (Sec59p) and dolichyl phosphate mannose (DolPMan) synthase (Dpm1p), have an aberrant cell wall structure and composition.
      Title: Defect in dolichol-dependent glycosylation increases sensitivity of Saccharomyces cerevisiae towards anti-fungal drugs.
    2. S. cerevisiae DPMS activity is regulated by the cAMP-dependent protein phosphorylation signal, and the phosphorylation target is serine 141
      Title: In vitro phosphorylation by cAMP-dependent protein kinase up-regulates recombinant Saccharomyces cerevisiae mannosylphosphodolichol synthase.
    3. A three-dimensional (3D) model of the enzyme was produced with bound substrates, Dol-P, GDP-Man, and divalent cations to delineate the binding sites for these substrates as well as the catalytic site.
      Title: Structural studies and mechanism of Saccharomyces cerevisiae dolichyl-phosphate-mannose synthase: insights into the initial step of synthesis of dolichyl-phosphate-linked oligosaccharide chains in membranes of endoplasmic reticulum.
    4. Interaction of Dpm1p with Sac1p persists during exponential cell division but is rapidly abolished when cell growth slows.
      Title: Cell growth-dependent coordination of lipid signaling and glycosylation is mediated by interactions between Sac1p and Dpm1p.
    5. Evidence is presented that temperature-sensitive Saccharomyces cerevisiae mutants, impaired in dolichol kinase (Sec59p) or dolichyl phosphate mannose synthase (Dpm1p) activity have an aberrant cell wall composition and ultrastructure.
      Title: Dissecting the role of dolichol in cell wall assembly in the yeast mutants impaired in early glycosylation reactions.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables dolichyl-phosphate beta-D-mannosyltransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables dolichyl-phosphate beta-D-mannosyltransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables dolichyl-phosphate beta-D-mannosyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables glycosyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in GPI anchor biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in GPI anchor biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in dolichol-linked oligosaccharide biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in dolichol-linked oligosaccharide biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein O-linked mannosylation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in protein glycosylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cell periphery HDA PubMed 
    located_in endoplasmic reticulum HDA PubMed 
    located_in endoplasmic reticulum IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in endoplasmic reticulum IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in endoplasmic reticulum membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in endoplasmic reticulum membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in mitochondrial outer membrane HDA PubMed 
    located_in mitochondrion HDA PubMed 
    located_in nuclear outer membrane-endoplasmic reticulum membrane network IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    dolichyl-phosphate beta-D-mannosyltransferase
    NP_015509.1
    • Dolichol phosphate mannose (Dol-P-Man) synthase of ER membrane; catalyzes formation of Dol-P-Man from Dol-P and GDP-Man; required for biosynthesis of glycosyl phosphatidylinositol (GPI) membrane anchor, as well as O-mannosylation and protein N- and O-linked glycosylation; human homolog DPM1 can complement yeast mutant strains

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001148.4 Reference assembly

      Range
      900755..901558
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001184280.1 → NP_015509.1  TPA: dolichyl-phosphate beta-D-mannosyltransferase [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_015509.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6W4I3, P14020
      UniProtKB/TrEMBL
      A6ZX57, B3LKE2, C7GP65, C8ZJL2, G2WPZ4, N1NW70
      Conserved Domains (1) summary
      cd06442
      Location:7 → 234
      DPM1_like; DPM1_like represents putative enzymes similar to eukaryotic DPM1
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P14020.3

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