Me1 malic enzyme 1, NADP(+)-dependent, cytosolic [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Me1provided by MGI
Official Full Name: malic enzyme 1, NADP(+)-dependent, cytosolicprovided by MGI
Primary source: MGI:MGI:97043
See related: Ensembl:ENSMUSG00000032418 AllianceGenome:MGI:97043
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Mod1; Mdh-1; Mod-1; D9Ertd267e
Summary: Enables malate dehydrogenase (decarboxylating) (NADP+) activity. Predicted to be involved in several processes, including carboxylic acid metabolic process; nicotinamide nucleotide metabolic process; and protein homotetramerization. Located in mitochondrion. Is expressed in several structures, including alimentary system; cardiovascular system; genitourinary system; hemolymphoid system gland; and nervous system. Orthologous to human ME1 (malic enzyme 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Broad expression in subcutaneous fat pad adult (RPKM 140.3), mammary gland adult (RPKM 130.2) and 16 other tissues See more
Orthologs: human all
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Genomic context

Location:: 9 E3.1; 9 46.58 cM

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	9	NC_000075.7 (86463416..86577967, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	9	NC_000075.6 (86581363..86695914, complement)

Chromosome 9 - NC_000075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Malic enzyme 1 knockout has no deleterious phenotype and is favored in the male germline under standard laboratory conditions.
Title: Malic enzyme 1 knockout has no deleterious phenotype and is favored in the male germline under standard laboratory conditions.
Malic Enzyme 1 (ME1) Promotes Adiposity and Hepatic Steatosis and Induces Circulating Insulin and Leptin in Obese Female Mice.
Title: Malic Enzyme 1 (ME1) Promotes Adiposity and Hepatic Steatosis and Induces Circulating Insulin and Leptin in Obese Female Mice.
Malic enzyme 1 is important for uterine decidualization in response to progesterone/cAMP/PKA/HB-EGF pathway.
Title: Malic enzyme 1 is important for uterine decidualization in response to progesterone/cAMP/PKA/HB-EGF pathway.
Data suggest that Ad4BP plays role in regulation of intracellular NADPH concentration via transcription of Me1 and Mthfd2 genes in adrenocortical cells. (Ad4BP = nuclear receptor subfamily 5 group A member 1; Me1 = malic enzyme 1; Mthfd2 = bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase)
Title: Role of Ad4-binding protein/steroidogenic factor 1 in regulating NADPH production in adrenocortical Y-1 cells.
This study showed that (2)H-labeled tracers enable dissection of NADPH production routes across cell types and environmental conditions.
Title: Malic enzyme tracers reveal hypoxia-induced switch in adipocyte NADPH pathway usage.
ME1 expression was found to be mutant-KRAS-associated in an NSCLC mouse model.
Title: Mutant KRAS associated malic enzyme 1 expression is a predictive marker for radiation therapy response in non-small cell lung cancer.
p53 represses the expression of the tricarboxylic-acid-cycle-associated malic enzymes ME1 and ME2 in human and mouse cells
Title: Reciprocal regulation of p53 and malic enzymes modulates metabolism and senescence.
although ME1 overexpression augments anaplerosis and glucose stimulated insulin secretion in INS-1 832/13 cells, it is not likely involved in methyl succinate and glucose stimulated insulin secretion in pancreatic islets
Title: Role for malic enzyme, pyruvate carboxylation, and mitochondrial malate import in glucose-stimulated insulin secretion.
cytosolic or mitochondrial isoforms of malic enzyme do not affect glucose-stimulated insulin secretion from rodent islets
Title: Silencing of cytosolic or mitochondrial isoforms of malic enzyme has no effect on glucose-stimulated insulin secretion from rodent islets.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (4)
Spontaneous (1) 1 citation
Targeted (2) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Mod1 (e-PCR), detects polymorphism
- UniSTS:143891

Mod1 (e-PCR), detects polymorphism
- UniSTS:143892

D9Mit135 (e-PCR), detects polymorphism
- UniSTS:116772

Me1 (e-PCR), detects polymorphism
- UniSTS:525990

RH126686 (e-PCR)
- UniSTS:167602

D9Ertd305e (e-PCR), detects polymorphism
- UniSTS:167607

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables NAD binding	IEA Inferred from Electronic Annotation more info
enables NADP binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables magnesium ion binding	ISO Inferred from Sequence Orthology more info
enables magnesium ion binding	ISS Inferred from Sequence or Structural Similarity more info
enables malate dehydrogenase (decarboxylating) (NADP+) activity	IBA Inferred from Biological aspect of Ancestor more info
enables malate dehydrogenase (decarboxylating) (NADP+) activity	IDA Inferred from Direct Assay more info	PubMed
enables malate dehydrogenase (decarboxylating) (NADP+) activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables malate dehydrogenase (decarboxylating) (NADP+) activity	ISO Inferred from Sequence Orthology more info
enables malate dehydrogenase (decarboxylating) (NADP+) activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables malic enzyme activity	ISO Inferred from Sequence Orthology more info
enables malic enzyme activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables manganese ion binding	ISO Inferred from Sequence Orthology more info
enables manganese ion binding	ISS Inferred from Sequence or Structural Similarity more info
enables oxaloacetate decarboxylase activity	ISO Inferred from Sequence Orthology more info
enables oxaloacetate decarboxylase activity	ISS Inferred from Sequence or Structural Similarity more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in NADH metabolic process	IEA Inferred from Electronic Annotation more info
involved_in NADH metabolic process	ISO Inferred from Sequence Orthology more info
involved_in NADP metabolic process	IEA Inferred from Electronic Annotation more info
involved_in NADP metabolic process	ISO Inferred from Sequence Orthology more info
involved_in malate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in malate metabolic process	ISO Inferred from Sequence Orthology more info
involved_in malate metabolic process	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in protein homotetramerization	ISO Inferred from Sequence Orthology more info
involved_in protein homotetramerization	ISS Inferred from Sequence or Structural Similarity more info
involved_in pyruvate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of NADP metabolic process	IEA Inferred from Electronic Annotation more info
involved_in regulation of NADP metabolic process	ISO Inferred from Sequence Orthology more info
involved_in response to hormone	ISO Inferred from Sequence Orthology more info
involved_in response to hormone	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISS Inferred from Sequence or Structural Similarity more info
located_in mitochondrion	HDA more info	PubMed
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: NADP-dependent malic enzyme

Names: NADP-ME; malic enzyme, supernatant

NP_001185862.1

EC 1.1.1.40

NP_032641.2

EC 1.1.1.40

XP_011240968.1

EC 1.1.1.40

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001198933.1 → NP_001185862.1 NADP-dependent malic enzyme isoform 2

See identical proteins and their annotated locations for NP_001185862.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) has an alternate exon at its 5' UTR and 5' coding region, compared to variant 1, which results in a protein (isoform 2) with a shorter and distinct N-terminus, compared to isoform 1.

Source sequence(s)

AK077968, AK163403, AV363687

Consensus CDS

CCDS81047.1

UniProtKB/TrEMBL

Q3TQP6, Q921S3

Related

ENSMUSP00000140887.2, ENSMUST00000185374.2

Conserved Domains (3) summary

cd05312
Location:250 → 527

NAD_bind_1_malic_enz; NAD(P) binding domain of malic enzyme (ME), subgroup 1

PLN03129
Location:7 → 531

PLN03129; NADP-dependent malic enzyme; Provisional

pfam00390
Location:59 → 240

malic; Malic enzyme, N-terminal domain
NM_008615.2 → NP_032641.2 NADP-dependent malic enzyme isoform 1

See identical proteins and their annotated locations for NP_032641.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longer isoform (1).

Source sequence(s)

AK077968, AV363687, CJ070715

Consensus CDS

CCDS23383.1

UniProtKB/Swiss-Prot

P06801, Q9DBF9

UniProtKB/TrEMBL

Q921S3

Related

ENSMUSP00000034989.9, ENSMUST00000034989.15

Conserved Domains (3) summary

cd05312
Location:270 → 547

NAD_bind_1_malic_enz; NAD(P) binding domain of malic enzyme (ME), subgroup 1

PLN03129
Location:2 → 551

PLN03129; NADP-dependent malic enzyme; Provisional

pfam00390
Location:79 → 260

malic; Malic enzyme, N-terminal domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000075.7 Reference GRCm39 C57BL/6J

Range

86463416..86577967 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011242666.2 → XP_011240968.1 NADP-dependent malic enzyme isoform X1

UniProtKB/TrEMBL

Q921S3

Conserved Domains (1) summary

PLN03129
Location:1 → 476

PLN03129; NADP-dependent malic enzyme; Provisional