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    WSS1 metalloendopeptidase WSS1 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 856536, updated on 2-Nov-2024

    Summary

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    Official Symbol
    WSS1
    Official Full Name
    metalloendopeptidase WSS1
    Primary source
    SGD:S000001176
    Locus tag
    YHR134W
    See related
    AllianceGenome:SGD:S000001176; FungiDB:YHR134W; VEuPathDB:YHR134W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables SUMO binding activity; SUMO ligase activity; and metalloendopeptidase activity. Involved in DNA repair; histone catabolic process; and protein sumoylation. Located in fungal-type vacuole and nuclear envelope. [provided by Alliance of Genome Resources, Nov 2024]
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    Genomic context

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    See WSS1 in Genome Data Viewer
    Location:
    chromosome: VIII
    Exon count:
    1
    Sequence:
    Chromosome: VIII; NC_001140.6 (371749..372558)

    Chromosome VIII - NC_001140.6Genomic Context describing neighboring genes Neighboring gene phosphatase regulator Neighboring gene Nsg1p Neighboring gene serine/threonine protein kinase YCK1 Neighboring gene Spl2p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001140.6

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Structural analysis of Wss1 protein from saccharomyces cerevisiae. Yang X, et al. Sci Rep, 2017 Aug 15. PMID 28811590, Free PMC Article
    2. The Saccharomyces cerevisiae Wss1 protein is only present in mother cells. van Heusden GP, et al. FEMS Microbiol Lett, 2008 May. PMID 18336552
    3. The yeast proteases Ddi1 and Wss1 are both involved in the DNA replication stress response. Svoboda M, et al. DNA Repair (Amst), 2019 Aug. PMID 31276951
    4. DNA-protein crosslink repair: proteases as DNA repair enzymes. Stingele J, et al. Trends Biochem Sci, 2015 Feb. PMID 25496645
    5. Cleavage-defective Topoisomerase I mutants sharply increase G-quadruplex-associated genomic instability. Berroyer A, et al. Microb Cell, 2022 Mar 7. PMID 35291312, Free PMC Article

    See all (68) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The yeast proteases Ddi1 and Wss1 are both involved in the DNA replication stress response.
      Title: The yeast proteases Ddi1 and Wss1 are both involved in the DNA replication stress response.
    2. e show that SPRTN is a DNA-dependent mammalian protease required for resolving cytotoxic DNA-protein crosslinks (DPCs)- a function that had only been attributed to the metalloprotease Wss1 in budding yeast. We provide genetic evidence that SPRTN and Wss1 function distinctly in vivo to resolve DPCs
      Title: SPRTN is a mammalian DNA-binding metalloprotease that resolves DNA-protein crosslinks.
    3. Upon DNA damage Wss1/Cdc48/Doa1 ternary complex is recruited to sumoylated targets and catalyzes SUMO chain extension through a newly recognized SUMO ligase activity.
      Title: Wss1 metalloprotease partners with Cdc48/Doa1 in processing genotoxic SUMO conjugates.
    4. Data suggest that Wss1 contributes to survival of DNA-protein crosslink (DPC)-harboring cells by acting on DPCs proteolytically. Wss1 enables replication of DPC-containing DNA and thus promotes genome integrity.
      Title: A DNA-dependent protease involved in DNA-protein crosslink repair.
    5. In dividing cells, Wss1 is exclusively present in the mother cell.
      Title: The Saccharomyces cerevisiae Wss1 protein is only present in mother cells.
    6. Involement of WSS1 in the DNA damage response.
      Title: Coordinated functions of WSS1, PSY2 and TOF1 in the DNA damage response.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables SUMO binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables SUMO ligase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metalloendopeptidase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metalloendopeptidase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables metallopeptidase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables metallopeptidase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metallopeptidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables zinc ion binding RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA damage response IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA repair IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in histone catabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein autosumoylation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein sumoylation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein sumoylation IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in proteolysis IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in replication-born double-strand break repair via sister chromatid exchange IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in translesion synthesis IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in translesion synthesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in fungal-type vacuole IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nuclear envelope IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    metalloendopeptidase WSS1
    NP_012002.1
    • SUMO-ligase and SUMO-targeted metalloprotease; involved in DNA repair; removes DNA-protein crosslinks at stalled replication forks during replication of damaged DNA; clears chromatin-bound sumoylated proteins; localizes to single spot on nuclear periphery of mother cells but not daughters; exhibits vacuolar localization upon genotoxic stress; activated by DNA binding; member of minigluzincins protease family with mammalian DVC1/Spartan

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001140.6 Reference assembly

      Range
      371749..372558
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001179264.1 → NP_012002.1  TPA: metalloendopeptidase WSS1 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_012002.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D3DL83, P38838
      UniProtKB/TrEMBL
      B3LSP8, C7GTW1, C8Z9S9, N1P2U5
      Conserved Domains (1) summary
      pfam08325
      Location:27 → 221
      WLM; WLM domain
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P38838.1

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