DID2 Did2p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: DID2
Official Full Name: Did2p
Primary source: SGD:S000006435
Locus tag: YKR035W-A
See related: AllianceGenome:SGD:S000006435; FungiDB:YKR035W-A; VEuPathDB:YKR035W-A
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CHM1; FTI1; VPL30; VPS46
Summary: Involved in ESCRT III complex assembly; late endosome to vacuole transport via multivesicular body sorting pathway; and protein targeting to vacuole. Located in late endosome. Human ortholog(s) of this gene implicated in pontocerebellar hypoplasia type 8. Orthologous to several human genes including CHMP1B (charged multivesicular body protein 1B). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See DID2 in Genome Data Viewer

Location:: chromosome: XI

Exon count:: 1

Sequence:: Chromosome: XI; NC_001143.9 (507939..508553)

Chromosome XI - NC_001143.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001143.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

the Did2 binding does not result in Vta1NTD significant conformational changes, revealing that Did2, similar to Vps60, enhances Vta1 stimulation of Vps4 ATPase activity in an indirect manner.
Title: NMR studies on the interactions between yeast Vta1 and Did2 during the multivesicular bodies sorting pathway.
the ESCRT regulator Did2 coordinates precise maturation of endosomes and thus provides the correct membrane identity for efficient endosomal long-distance transport.
Title: The ESCRT regulator Did2 maintains the balance between long-distance endosomal transport and endocytic trafficking.
Cocrystallization of Ist1NTD with a Did2 fragment shows that Ist1 interacts with the Did2 C-terminal MIM1 (MIT-interacting motif 1) via a novel MIM-binding structural motif.
Title: Structural basis of Ist1 function and Ist1-Did2 interaction in the multivesicular body pathway and cytokinesis.
A model in which the Ist1-Did2 and Vta1-Vps60 complexes independently modulate late steps in the multivesicular body-sorting pathway, is proposed.
Title: Novel Ist1-Did2 complex functions at a late step in multivesicular body sorting.
Vps46p regulates the membrane association of Vps4p and is required for the interaction of Vta1p with Vps32p/Snf7p of the ESCRT-III complex.
Title: Vta1p and Vps46p regulate the membrane association and ATPase activity of Vps4p at the yeast multivesicular body.
We show that Did2 directs Vps4 activity to the dissociation of ESCRT-III but has no role in the dissociation of ESCRT-I or -II.
Title: Did2 coordinates Vps4-mediated dissociation of ESCRT-III from endosomes.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables molecular_function	ND No biological Data available more info

Process	Evidence Code	Pubs
involved_in ESCRT III complex assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in endosome transport via multivesicular body sorting pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in late endosome to vacuole transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in late endosome to vacuole transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in late endosome to vacuole transport via multivesicular body sorting pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein targeting to vacuole	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein transport	IEA Inferred from Electronic Annotation more info
involved_in vacuolar transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of ESCRT III complex	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in endomembrane system	IEA Inferred from Electronic Annotation more info
located_in endosome	IEA Inferred from Electronic Annotation more info
located_in endosome membrane	IEA Inferred from Electronic Annotation more info
located_in intracellular membrane-bounded organelle	IEA Inferred from Electronic Annotation more info
located_in late endosome	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in multivesicular body	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: Did2p

NP_012961.3

Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001143.9 Reference assembly

Range

507939..508553

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001184309.3 → NP_012961.3 TPA: Did2p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012961.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VX98, P69771

UniProtKB/TrEMBL

A6ZZZ7, B3LRB6, B5VMI3, C7GNM7, C8ZCK0, G2WI65, N1NZG8

Conserved Domains (1) summary

COG5491
Location:21 → 204

Did4; Archaeal division protein CdvB, Snf7/Vps24/ESCRT-III family [Cell cycle control, cell division, chromosome partitioning]