GPA1 guanine nucleotide-binding protein subunit alpha [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: GPA1
Official Full Name: guanine nucleotide-binding protein subunit alpha
Primary source: SGD:S000001047
Locus tag: YHR005C
See related: AllianceGenome:SGD:S000001047; FungiDB:YHR005C; VEuPathDB:YHR005C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CDC70; DAC1; SCG1
Summary: Enables G-protein beta-subunit binding activity and GTPase activity. Involved in several processes, including conjugation with cellular fusion; regulation of MAPK export from nucleus; and response to pheromone triggering conjugation with cellular fusion. Located in endosome and plasma membrane. Part of heterotrimeric G-protein complex. Used to study McCune Albright syndrome. Human ortholog(s) of this gene implicated in color blindness (multiple); developmental and epileptic encephalopathy 17; neurodevelopmental disorder with involuntary movements; and night blindness (multiple). Orthologous to several human genes including GNAI1 (G protein subunit alpha i1); GNAI2 (G protein subunit alpha i2); and GNAO1 (G protein subunit alpha o1). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See GPA1 in Genome Data Viewer

Location:: chromosome: VIII

Exon count:: 1

Sequence:: Chromosome: VIII; NC_001140.6 (113499..114917, complement)

Chromosome VIII - NC_001140.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001140.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Interaction between Ste2p and Gpa1p takes palce before ligand binding via the Ste2p C-terminal domain and the Gpa1p N-terminal domain.
Title: GPCR-Gα protein precoupling: Interaction between Ste2p, a yeast GPCR, and Gpa1p, its Gα protein, is formed before ligand binding via the Ste2p C-terminal domain and the Gpa1p N-terminal domain.
Data indicate that proper endocytosis of G protein alpha subunit Gpa1 is required for sustained morphogenesis and efficient mating.
Title: Guanine nucleotide-binding protein (Gα) endocytosis by a cascade of ubiquitin binding domain proteins is required for sustained morphogenesis and proper mating in yeast.
A mutant gpa1 protein is biochemically different and still physiologically relevant.
Title: Soya bean Gα proteins with distinct biochemical properties exhibit differential ability to complement Saccharomyces cerevisiae gpa1 mutant.
the same kinases and phosphatase that regulate Snf1 also regulate Gpa1.
Title: Regulation of yeast G protein signaling by the kinases that activate the AMPK homolog Snf1.
Gpa1 serves as a positional determinant for Kar3-bound microtubule plus ends during mating.
Title: The mating-specific Galpha interacts with a kinesin-14 and regulates pheromone-induced nuclear migration in budding yeast.
the ubiquitin ligase Rsp5 is necessary for Gpa1 mono-ubiquitination in vivo and that the purified enzyme is sufficient to catalyze Gpa1 mono-ubiquitination in vitro.
Title: G Protein Mono-ubiquitination by the Rsp5 Ubiquitin Ligase.
These results indicate that chemoattractant-induced cell polarization requires continuous receptor-Galphabetagamma communication but not modulation of MAP kinase signaling.
Title: Distinct roles for two Galpha-Gbeta interfaces in cell polarity control by a yeast heterotrimeric G protein.
Study introduced the McCune-Albright syndrome (MAS) mutation into the analogous site in yeast gene, GPA1 and randomly mutated the gene to produce intragenic suppressors; a mutation in GPA1, is an intragenic suppressor of the MAS defect.
Title: Identification of a second-site suppressor mutation of the GTPase defect associated with McCune-Albright syndrome: a model using the yeast heterotrimeric G protein, GPA1.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables G protein-coupled receptor binding	IBA Inferred from Biological aspect of Ancestor more info
enables G protein-coupled receptor binding	IEA Inferred from Electronic Annotation more info
enables G-protein beta-subunit binding	IPI Inferred from Physical Interaction more info	PubMed
enables G-protein beta/gamma-subunit complex binding	IBA Inferred from Biological aspect of Ancestor more info
enables G-protein beta/gamma-subunit complex binding	IEA Inferred from Electronic Annotation more info
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTPase activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activity	IDA Inferred from Direct Assay more info	PubMed
enables GTPase activity	IEA Inferred from Electronic Annotation more info
enables GTPase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in karyogamy involved in conjugation with cellular fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nuclear migration involved in conjugation with cellular fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pheromone-dependent signal transduction involved in conjugation with cellular fusion	IBA Inferred from Biological aspect of Ancestor more info
involved_in pheromone-dependent signal transduction involved in conjugation with cellular fusion	IGI Inferred from Genetic Interaction more info	PubMed
involved_in pheromone-dependent signal transduction involved in conjugation with cellular fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of carbohydrate metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to pheromone	IEA Inferred from Electronic Annotation more info
involved_in signal transduction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytosol	HDA more info	PubMed
located_in cytosol	HDA more info	PubMed
located_in endosome	IDA Inferred from Direct Assay more info	PubMed
located_in endosome	IEA Inferred from Electronic Annotation more info
located_in endosome membrane	IEA Inferred from Electronic Annotation more info
part_of heterotrimeric G-protein complex	IBA Inferred from Biological aspect of Ancestor more info
part_of heterotrimeric G-protein complex	IDA Inferred from Direct Assay more info	PubMed
part_of heterotrimeric G-protein complex	IEA Inferred from Electronic Annotation more info
part_of heterotrimeric G-protein complex	IMP Inferred from Mutant Phenotype more info	PubMed
part_of heterotrimeric G-protein complex	IPI Inferred from Physical Interaction more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: guanine nucleotide-binding protein subunit alpha

NP_011868.1

Subunit of G protein involved in pheromone response; GTP-binding alpha subunit of heterotrimeric G protein; negatively regulates mating pathway by sequestering G(beta)gamma and triggering an adaptive response; activates Vps34p at endosome; protein abundance increases in response to DNA replication stress; mutations in human homolog GNAS associated with McCune-Albright syndrome

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001140.6 Reference assembly

Range

113499..114917 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179135.1 → NP_011868.1 TPA: guanine nucleotide-binding protein subunit alpha [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011868.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DKU8, P08539

UniProtKB/TrEMBL

B3LSC3

Conserved Domains (1) summary

cd00066
Location:42 → 466

G-alpha; Alpha subunit of G proteins (guanine nucleotide binding)