PCH2 Pch2p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: PCH2
Official Full Name: Pch2p
Primary source: SGD:S000000390
Locus tag: YBR186W
See related: AllianceGenome:SGD:S000000390; FungiDB:YBR186W; VEuPathDB:YBR186W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables ATP hydrolysis activity. Involved in meiotic DNA double-strand break formation; meiotic recombination checkpoint signaling; and reciprocal meiotic recombination. Located in nucleolus. Human ortholog(s) of this gene implicated in mosaic variegated aneuploidy syndrome 3. Orthologous to human TRIP13 (thyroid hormone receptor interactor 13). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See PCH2 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 2

Sequence:: Chromosome: II; NC_001134.8 (600553..602360)

Chromosome II - NC_001134.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Pch2 orchestrates the meiotic recombination checkpoint from the cytoplasm.
Title: Pch2 orchestrates the meiotic recombination checkpoint from the cytoplasm.
Getting there: understanding the chromosomal recruitment of the AAA+ ATPase Pch2/TRIP13 during meiosis.
Title: Getting there: understanding the chromosomal recruitment of the AAA+ ATPase Pch2/TRIP13 during meiosis.
Homeostatic Control of Meiotic Prophase Checkpoint Function by Pch2 and Hop1.
Title: Homeostatic Control of Meiotic Prophase Checkpoint Function by Pch2 and Hop1.
Biochemical and functional characterization of a meiosis-specific Pch2/ORC AAA+ assembly.
Title: Biochemical and functional characterization of a meiosis-specific Pch2/ORC AAA+ assembly.
Active transcription and Orc1 drive chromatin association of the AAA+ ATPase Pch2 during meiotic G2/prophase.
Title: Active transcription and Orc1 drive chromatin association of the AAA+ ATPase Pch2 during meiotic G2/prophase.
pch2Delta showed approximately 50% genome-wide increase in crossover frequency, elevated noncrossovers and loss of chromosome size dependent double-strand break formation.
Title: Modulating Crossover Frequency and Interference for Obligate Crossovers in Saccharomyces cerevisiae Meiosis.
discuss our current understanding of the function of Pch2(TRIP13) during meiotic and mitotic cell divisions, with a focus on its enzymatic role towards HORMA domain-containing clients
Title: Pch2(TRIP13): controlling cell division through regulation of HORMA domains.
Pch2 prevents Mec1/Tel1-mediated Hop1 phosphorylation occurring independently of Red1 in Saccharomyces cerevisiae meiosis.
Title: Pch2 prevents Mec1/Tel1-mediated Hop1 phosphorylation occurring independently of Red1 in budding yeast meiosis.
Pch2 displaces Hop1 from large DNA substrates and both ATP binding and hydrolysis by Pch2 are required for Pch2-Hop1 transactions
Title: Pch2 is a hexameric ring ATPase that remodels the chromosome axis protein Hop1.
interaction with Xrs2 may enable Pch2 to remodel chromosome structure adjacent to the site of a DSB and thereby promote accessibility of Hop1 to the Tel1 kinase
Title: Pch2 acts through Xrs2 and Tel1/ATM to modulate interhomolog bias and checkpoint function during meiosis.

Submit: New GeneRIF Correction See all GeneRIFs (16)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in meiotic DNA double-strand break formation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in meiotic DNA double-strand break formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in meiotic recombination checkpoint signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in meiotic recombination checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in reciprocal meiotic recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in reciprocal meiotic recombination	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in chromosome	IBA Inferred from Biological aspect of Ancestor more info
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in nucleolus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleolus	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Pch2p

NP_009745.2

Hexameric ring ATPase that remodels chromosome axis protein Hop1p; nucleolar component of the pachytene checkpoint, which prevents chromosome segregation when recombination and chromosome synapsis are defective; also represses meiotic interhomolog recombination in rDNA; required for meiotic double-stranded break formation

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001134.8 Reference assembly

Range

600553..602360

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178534.1 → NP_009745.2 TPA: Pch2p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009745.2

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VQI1, P38126

UniProtKB/TrEMBL

G2W9I8, N1PAU6

Conserved Domains (2) summary

COG0464
Location:1 → 487

SpoVK; AAA+-type ATPase, SpoVK/Ycf46/Vps4 family [Cell wall/membrane/envelope biogenesis, Cell cycle control, cell division, chromosome partitioning, Signal transduction mechanisms]

cl38936
Location:248 → 465

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases