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    RIB2 bifunctional DRAP deaminase/tRNA pseudouridine synthase RIB2 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 854088, updated on 2-Nov-2024

    Summary

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    Official Symbol
    RIB2
    Official Full Name
    bifunctional DRAP deaminase/tRNA pseudouridine synthase RIB2
    Primary source
    SGD:S000005427
    Locus tag
    YOL066C
    See related
    AllianceGenome:SGD:S000005427; FungiDB:YOL066C; VEuPathDB:YOL066C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    PUS8
    Summary
    Enables diaminohydroxyphosphoribosylaminopyrimidine deaminase activity and pseudouridine synthase activity. Involved in riboflavin biosynthetic process and tRNA pseudouridine synthesis. Located in cytoplasm. Orthologous to human RPUSD2 (RNA pseudouridine synthase domain containing 2). [provided by Alliance of Genome Resources, Nov 2024]
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    Genomic context

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    See RIB2 in Genome Data Viewer
    Location:
    chromosome: XV
    Exon count:
    1
    Sequence:
    Chromosome: XV; NC_001147.6 (202696..204471, complement)

    Chromosome XV - NC_001147.6Genomic Context describing neighboring genes Neighboring gene histone deacetylase HST1 Neighboring gene Rtg1p Neighboring gene phosphoinositide 5-phosphatase INP54 Neighboring gene 3'(2'),5'-bisphosphate nucleotidase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001147.6

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Biosynthesis of riboflavin. Formation of 2,5-diamino-6-hydroxy-4-(1'-D-ribitylamino)pyrimidine in a riboflavin auxotroph. Bacher A, et al. J Biol Chem, 1970 Sep 25. PMID 5456141
    2. Optimisation of expression and purification of the recombinant Yol066 (Rib2) protein from Saccharomyces cerevisiae. Urban A, et al. J Chromatogr B Analyt Technol Biomed Life Sci, 2003 Mar 25. PMID 12651014
    3. Evolution of vitamin B2 biosynthesis: eubacterial RibG and fungal Rib2 deaminases. Chen SC, et al. Acta Crystallogr D Biol Crystallogr, 2013 Feb. PMID 23385458
    4. Pseudouridylation at position 32 of mitochondrial and cytoplasmic tRNAs requires two distinct enzymes in Saccharomyces cerevisiae. Behm-Ansmant I, et al. J Biol Chem, 2004 Dec 17. PMID 15466869
    5. Biosynthesis of riboflavine in Saccharomyces cerevisiae: the role of genes rib 1 and rib 7 . Oltmanns O, et al. J Bacteriol, 1972 Jun. PMID 4555411, Free PMC Article

    See all (28) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. two enzymes, Rib2/Pus8p and Pus9p, are required for Psi32 formation in cytoplasmic and mitochondrial tRNAs, respectively
      Title: Pseudouridylation at position 32 of mitochondrial and cytoplasmic tRNAs requires two distinct enzymes in Saccharomyces cerevisiae.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables RNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables deaminase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables diaminohydroxyphosphoribosylaminopyrimidine deaminase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables diaminohydroxyphosphoribosylaminopyrimidine deaminase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables isomerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables pseudouridine synthase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables pseudouridine synthase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables pseudouridine synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables pseudouridine synthase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables tRNA pseudouridine(32) synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables zinc ion binding RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in enzyme-directed rRNA pseudouridine synthesis IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in nucleobase-containing compound biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in pseudouridine synthesis IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in riboflavin biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in riboflavin biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in tRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tRNA pseudouridine synthesis IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in tRNA pseudouridine synthesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm HDA PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    bifunctional DRAP deaminase/tRNA pseudouridine synthase RIB2
    NP_014575.1
    • Bifunctional DRAP deaminase tRNA:pseudouridine synthase; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs; RIB2 has a paralog, PUS9, that arose from the whole genome duplication

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001147.6 Reference assembly

      Range
      202696..204471 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001183321.1 → NP_014575.1  TPA: bifunctional DRAP deaminase/tRNA pseudouridine synthase RIB2 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_014575.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6W201, Q12362
      UniProtKB/TrEMBL
      A6ZNE4, B3LIZ4, C7GK33, N1NZ56
      Conserved Domains (2) summary
      TIGR00005
      Location:94 → 406
      rluA_subfam; pseudouridine synthase, RluA family
      pfam18785
      Location:459 → 580
      Inv-AAD; Invertebrate-AID/APOBEC-deaminase
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q12362.1

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