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    APE1 metalloaminopeptidase APE1 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 853758, updated on 17-May-2024

    Summary

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    Gene symbol
    APE1
    Gene description
    metalloaminopeptidase APE1
    Primary source
    FungiDB:YKL103C
    Locus tag
    YKL103C
    See related
    SGD:S000001586; AllianceGenome:SGD:S000001586; VEuPathDB:YKL103C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    API; LAP4; YSC1
    Summary
    Enables metalloaminopeptidase activity. Involved in cytoplasm to vacuole transport by the Cvt pathway. Located in fungal-type vacuole. Part of Cvt complex. Orthologous to human DNPEP (aspartyl aminopeptidase). [provided by Alliance of Genome Resources, Apr 2022]
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    Genomic context

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    See APE1 in Genome Data Viewer
    Location:
    chromosome: XI
    Exon count:
    1
    Sequence:
    Chromosome: XI; NC_001143.9 (246138..247682, complement)

    Chromosome XI - NC_001143.9Genomic Context describing neighboring genes Neighboring gene Seg2p Neighboring gene glutamine--fructose-6-phosphate transaminase (isomerizing) GFA1 Neighboring gene uncharacterized protein Neighboring gene uncharacterized protein Neighboring gene protein kinase HSL1

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001143.9

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. APE1/Ref-1 Role in Inflammation and Immune Response. Oliveira TT, et al. Front Immunol, 2022. PMID 35296074, Free PMC Article
    2. Structure of yeast Ape1 and its role in autophagic vesicle formation. Su MY, et al. Autophagy, 2015. PMID 26208681, Free PMC Article
    3. Lap4, a vacuolar aminopeptidase I, is involved in cadmium-glutathione metabolism. Adamis PD, et al. Biometals, 2009 Apr. PMID 18716881
    4. Crystallization of Saccharomyces cerevisiae aminopeptidase 1, the major cargo protein of the Cvt pathway. Adachi W, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2007 Mar 1. PMID 17329814, Free PMC Article
    5. The prepropeptide of vacuolar aminopeptidase I is necessary and sufficient to target the fluorescent reporter protein GFP to the vacuole of yeast by the Ccvt pathway. Martinez E, et al. Mol Microbiol, 1999 Jul. PMID 10411723

    See all (114) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. APE1/Ref-1 Role in Inflammation and Immune Response.
      Title: APE1/Ref-1 Role in Inflammation and Immune Response.
    2. Ape1 aggregates bind Atg19 and Atg8 in vitro; this could be used as a scaffold for an in vitro assay of autophagosome formation to elucidate the mechanisms of autophagy
      Title: Propeptide of aminopeptidase 1 protein mediates aggregation and vesicle formation in cytoplasm-to-vacuole targeting pathway.
    3. under cadmium stress, Lap4 and gamma-glutamyl transferase seem to work together to assure an efficient glutathione turnover stored in the vacuole
      Title: Lap4, a vacuolar aminopeptidase I, is involved in cadmium-glutathione metabolism.
    4. Mature Ape1 (mApe1) has been expressed, purified and crystallized in two crystal forms. Forms I and II contain 12 and four mApe1 molecules per asymmetric unit, respectively, and mApe1 exists as a tetrahedral dodecamer in both crystal forms.
      Title: Crystallization of Saccharomyces cerevisiae aminopeptidase 1, the major cargo protein of the Cvt pathway.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables aminopeptidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metalloaminopeptidase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables metalloaminopeptidase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metallopeptidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables zinc ion binding RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in cytoplasm to vacuole targeting by the Cvt pathway IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in proteolysis IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of Cvt complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm HDA PubMed 
    is_active_in fungal-type vacuole IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in fungal-type vacuole IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in vacuole IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    metalloaminopeptidase APE1
    NP_012819.1
    • Vacuolar aminopeptidase yscI; zinc metalloproteinase that belongs to peptidase family M18; often used as marker in studies of autophagy and cytosol to vacuole targeting (CVT) pathway; protein increases in abundance and relative distribution to cytoplasmic foci increases upon DNA replication stress; targeted to vacuole via Vps10p-dependent endosomal vacuolar protein sorting pathway and via CVT pathway

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001143.9 Reference assembly

      Range
      246138..247682 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001179669.1 → NP_012819.1  TPA: metalloaminopeptidase APE1 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_012819.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VXI5, P14904, P22060
      UniProtKB/TrEMBL
      A0A8H4C0C1, A6ZZL4, B3LQZ3, C7GVK8, C8ZC72, G2WHT1, N1NZ36
      Conserved Domains (1) summary
      cd05639
      Location:53 → 502
      M18; M18 peptidase aminopeptidase family
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P14904.2

    Gene LinkOut

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