arp3 actin-like protein Arp3 [Schizosaccharomyces pombe (fission yeast)] - Gene

Summary

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Gene symbol: arp3
Gene description: actin-like protein Arp3
Primary source: PomBase:SPAC630.03
Locus tag: SPAC630.03
See related: FungiDB:SPAC630.03; VEuPathDB:SPAC630.03
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Schizosaccharomyces pombe (strain: 972h-)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces
Also known as: act2
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Genomic context

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See arp3 in Genome Data Viewer

Location:: chromosome: I

Exon count:: 5

Sequence:: Chromosome: I; NC_003424.3 (350406..352296)

Chromosome I - NC_003424.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003424.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Mechanism of actin filament branch formation by Arp2/3 complex revealed by a high-resolution cryo-EM structureof the branch junction.
Title: Mechanism of actin filament branch formation by Arp2/3 complex revealed by a high-resolution cryo-EM structureof the branch junction.
Cryo-EM reveals the transition of Arp2/3 complex from inactive to nucleation-competent state.
Title: Cryo-EM reveals the transition of Arp2/3 complex from inactive to nucleation-competent state.
Force and phosphate release from Arp2/3 complex promote dissociation of actin filament branches.
Title: Force and phosphate release from Arp2/3 complex promote dissociation of actin filament branches.
WASp-VCA binding to Arp2/3 protein complex causes a conformational change that favors binding to the side of an actin filament, which allows further changes required to nucleate a daughter filament.
Title: Conformational changes in Arp2/3 complex induced by ATP, WASp-VCA, and actin filaments.
ARP3 role in the actin filament nucleation.ATP binding destabilizes this interaction via an allosteric link between the Arp3 nucleotide cleft and the hydrophobic groove, thereby promoting the short-pitch conformation.
Title: Identification of an ATP-controlled allosteric switch that controls actin filament nucleation by Arp2/3 complex.
Arp2/3 complex participates in regulating formin F-actin networks.
Title: Homeostatic actin cytoskeleton networks are regulated by assembly factor competition for monomers.
Insertions within the actin core of actin-related protein 3 (Arp3) modulate branching nucleation by Arp2/3 complex
Title: Insertions within the actin core of actin-related protein 3 (Arp3) modulate branching nucleation by Arp2/3 complex.
Cofilin rapidly dissociates Arp2/3 complex and branches by direct competition for binding sites on the actin filament and by propagation of structural changes in the actin filament that reduce affinity for Arp2/3 complex.
Title: Cofilin dissociates Arp2/3 complex and branches from actin filaments.
Truncation of the N terminus of ARPC5, the smallest subunit in the complex, increased the yield of Arp2Delta Arp2/3 complex during purification but did not compromise nucleation activity of the full Arp2/3 complex.
Title: Structure and biochemical properties of fission yeast Arp2/3 complex lacking the Arp2 subunit.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by PomBase

Function	Evidence Code	Pubs
enables ATP binding	IDA Inferred from Direct Assay more info	PubMed
enables actin filament binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in Arp2/3 complex-mediated actin nucleation	IBA Inferred from Biological aspect of Ancestor more info
involved_in Arp2/3 complex-mediated actin nucleation	IDA Inferred from Direct Assay more info	PubMed
involved_in actin cortical patch organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin filament polymerization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endocytosis	IC Inferred by Curator more info
involved_in mitotic cytokinesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of Arp2/3 protein complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Arp2/3 protein complex	IDA Inferred from Direct Assay more info	PubMed
is_active_in actin cortical patch	IBA Inferred from Biological aspect of Ancestor more info
is_active_in actin cortical patch	IDA Inferred from Direct Assay more info	PubMed
is_active_in cell cortex of cell tip	IDA Inferred from Direct Assay more info	PubMed
is_active_in cell division site	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	HDA more info	PubMed
is_active_in cytosol	HDA more info	PubMed

General protein information

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Preferred Names: actin-like protein Arp3

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003424.3 Reference assembly

Range

350406..352296

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001018298.2 → NP_592898.1 actin-like protein Arp3 [Schizosaccharomyces pombe]

See identical proteins and their annotated locations for NP_592898.1

Status: PROVISIONAL

UniProtKB/Swiss-Prot

P32390

Conserved Domains (1) summary

PTZ00280
Location:8 → 425

PTZ00280; Actin-related protein 3; Provisional