OPI3 bifunctional phosphatidyl-N-methylethanolamine N-methyltransferase/phosphatidyl-N-dimethylethanolamine N-methyltransferase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: OPI3
Gene description: bifunctional phosphatidyl-N-methylethanolamine N-methyltransferase/phosphatidyl-N-dimethylethanolamine N-methyltransferase
Primary source: FungiDB:YJR073C
Locus tag: YJR073C
See related: SGD:S000003834; AllianceGenome:SGD:S000003834; VEuPathDB:YJR073C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: PEM2
Summary: Enables phosphatidyl-N-dimethylethanolamine N-methyltransferase activity and phosphatidyl-N-methylethanolamine N-methyltransferase activity. Involved in phosphatidylcholine biosynthetic process. Located in cell periphery; endoplasmic reticulum; and mitochondrion. Orthologous to human PEMT (phosphatidylethanolamine N-methyltransferase). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See OPI3 in Genome Data Viewer

Location:: chromosome: X

Exon count:: 1

Sequence:: Chromosome: X; NC_001142.9 (572315..572935, complement)

Chromosome X - NC_001142.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001142.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Impairment of transcription factor Gcr1p binding motif perturbs OPI3 transcription in Saccharomyces cerevisiae.
Title: Impairment of transcription factor Gcr1p binding motif perturbs OPI3 transcription in Saccharomyces cerevisiae.
Phosphatidylcholine formation was observed not only in nanodiscs containing inserted Opi3 but also in nanodiscs devoid of the enzyme containing the lipid substrate. This result gives a clear indication for in trans catalysis by Opi3; i.e., it acts on the substrate in juxtaposed membranes, while in cis lipid conversion may also contribute.
Title: Lipid Conversion by Cell-Free Synthesized Phospholipid Methyltransferase Opi3 in Defined Nanodisc Membranes Supports an in Trans Mechanism.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables S-adenosylmethionine-dependent methyltransferase activity	IEA Inferred from Electronic Annotation more info
enables methyltransferase activity	IEA Inferred from Electronic Annotation more info
enables phosphatidyl-N-dimethylethanolamine N-methyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidyl-N-dimethylethanolamine N-methyltransferase activity	IEA Inferred from Electronic Annotation more info
enables phosphatidyl-N-dimethylethanolamine N-methyltransferase activity	IGI Inferred from Genetic Interaction more info	PubMed
enables phosphatidyl-N-dimethylethanolamine N-methyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables phosphatidyl-N-methylethanolamine N-methyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidyl-N-methylethanolamine N-methyltransferase activity	IEA Inferred from Electronic Annotation more info
enables phosphatidyl-N-methylethanolamine N-methyltransferase activity	IGI Inferred from Genetic Interaction more info	PubMed
enables phosphatidyl-N-methylethanolamine N-methyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables phosphatidylethanolamine N-methyltransferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in methylation	IEA Inferred from Electronic Annotation more info
involved_in phosphatidylcholine biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in phosphatidylcholine biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in phosphatidylcholine biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in phosphatidylcholine biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in phosphatidylcholine biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phospholipid biosynthetic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cell periphery	HDA more info	PubMed
located_in endoplasmic reticulum	HDA more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrial membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: bifunctional phosphatidyl-N-methylethanolamine N-methyltransferase/phosphatidyl-N-dimethylethanolamine N-methyltransferase

NP_012607.1

EC 2.1.1.17

EC 2.1.1.71

Methylene-fatty-acyl-phospholipid synthase; catalyzes the last two steps in phosphatidylcholine biosynthesis; also known as phospholipid methyltransferase

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001142.9 Reference assembly

Range

572315..572935 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181731.1 → NP_012607.1 TPA: bifunctional phosphatidyl-N-methylethanolamine N-methyltransferase/phosphatidyl-N-dimethylethanolamine N-methyltransferase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012607.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VWP3, P05375

UniProtKB/TrEMBL

A0A6A5PWF1, A6ZQ31, B3LQF9, B5VLM5, C7GMD1, C8ZBM0, G2WH77, N1P1M1

Conserved Domains (1) summary

pfam04191
Location:100 → 200

PEMT; Phospholipid methyltransferase