pfh1 DNA repair and recombination protein [Schizosaccharomyces pombe (fission yeast)] - Gene

Summary

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Official Symbol: pfh1
Official Full Name: DNA repair and recombination protein
Primary source: PomBase:SPBC887.14c
Locus tag: SPOM_SPBC887.14C
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Schizosaccharomyces pombe (strain: 972h-)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces
Also known as: pif1
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Genomic context

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See pfh1 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 2

Sequence:: Chromosome: II; NC_003423.3 (3567593..3570476, complement)

Chromosome II - NC_003423.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003423.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The unwinding of double-stranded DNA by Pif1 is dominated by highly processive and repetitive attempts of partial DNA opening.
Title: Branched unwinding mechanism of the Pif1 family of DNA helicases.
The nuclear isoform of Pfh1 (nPfh1) unwound RNA/DNA hybrids more efficiently than DNA/DNA. Pfh1 resolves RNA/DNA structures like R-loops in vivo. nPfh1 displaced proteins from DNA and possessed strand-annealing activity. The unwinding and protein displacement activities were dependent on the Pif1 signature motif. nPfh1-L430P and nPfh1-Delta21 still displayed binding to G-quadruplex DNA and strand-annealing.
Title: The Pif1 signature motif of Pfh1 is necessary for both protein displacement and helicase unwinding activities, but is dispensable for strand-annealing activity.
The nuclear isoform of Pfh1 (nPfh1) unwound both types of structure, and although the G4-stabilizing compound Phen-DC3 significantly enhanced their stability, nPfh1 still resolved them efficiently
Title: G-rich telomeric and ribosomal DNA sequences from the fission yeast genome form stable G-quadruplex DNA structures in vitro and are unwound by the Pfh1 DNA helicase.
In budding and fission yeasts, Pif1 family helicases impact both telomerase-mediated and semi-conservative replication of telomeric DNA as well as recombination-mediated telomere lengthening. (Review)
Title: Getting it done at the ends: Pif1 family DNA helicases and telomeres.
Pfh1 is an accessory DNA helicase that interacts with the replisome and promotes replication and suppresses DNA damage at hard-to-replicate sites.
Title: Pfh1 Is an Accessory Replicative Helicase that Interacts with the Replisome to Facilitate Fork Progression and Preserve Genome Integrity.
Pfh1 binds telomeric DNA.Pfh1 facilitates telomere replication.Pfh1 is a positive regulator of telomere length.
Title: The Pif1 family helicase Pfh1 facilitates telomere replication and has an RPA-dependent role during telomere lengthening.
The proteome of cells devoid of Pfh1 were analyzed and determined that gene products up- and down-regulated upon iron depletion in wild-type cells are constitutively misregulated in this mutant.
Title: Cells lacking pfh1, a fission yeast homolog of mammalian frataxin protein, display constitutive activation of the iron starvation response.
Pfh1 promotes DNA replication and separation of converged replication forks and suppresses DNA damage at hard-to-replicate sites.
Title: DNA replication through hard-to-replicate sites, including both highly transcribed RNA Pol II and Pol III genes, requires the S. pombe Pfh1 helicase.
These data demonstrate that Pfh1p has essential roles in the replication of both nuclear and mitochondrial DNA.
Title: The Schizosaccharomyces pombe Pfh1p DNA helicase is essential for the maintenance of nuclear and mitochondrial DNA.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by PomBase

Function	Evidence Code	Pubs
enables 5'-3' DNA helicase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 5'-3' DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables 5'-3' DNA/RNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	ISM Inferred from Sequence Model more info
enables ATP hydrolysis activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP-dependent protein-DNA complex displacement activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA/DNA annealing activity	IDA Inferred from Direct Assay more info	PubMed
enables G-quadruplex DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA binding	IDA Inferred from Direct Assay more info	PubMed
enables flap-structured DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables single-stranded DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables single-stranded DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA duplex unwinding	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA recombination	ISO Inferred from Sequence Orthology more info
involved_in DNA replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA strand elongation involved in mitotic DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in G-quadruplex DNA unwinding	IBA Inferred from Biological aspect of Ancestor more info
involved_in G-quadruplex DNA unwinding	IDA Inferred from Direct Assay more info	PubMed
involved_in G-quadruplex DNA unwinding	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondrial DNA repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondrial genome maintenance	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic recombination-dependent replication fork processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in removal of RNA primer involved in mitotic DNA replication	IGI Inferred from Genetic Interaction more info	PubMed
involved_in replication fork processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in replication fork reversal	IBA Inferred from Biological aspect of Ancestor more info
involved_in telomere maintenance	IBA Inferred from Biological aspect of Ancestor more info
involved_in telomere maintenance	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in chromatin	IDA Inferred from Direct Assay more info	PubMed
is_active_in chromosome, telomeric repeat region	IDA Inferred from Direct Assay more info	PubMed
is_active_in mitochondrial chromosome	IC Inferred by Curator more info
is_active_in mitochondrion	HDA more info	PubMed
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
is_active_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
is_active_in nuclear replication fork	IBA Inferred from Biological aspect of Ancestor more info
part_of nuclear replisome	EXP Inferred from Experiment more info	PubMed
is_active_in nucleolus	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	HDA more info	PubMed
is_active_in nucleus	IDA Inferred from Direct Assay more info	PubMed
is_active_in rDNA heterochromatin	IDA Inferred from Direct Assay more info	PubMed
is_active_in site of double-strand break	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: DNA repair and recombination protein

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003423.3 Reference assembly

Range

3567593..3570476 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001022408.3 → NP_596488.1 DNA repair and recombination protein [Schizosaccharomyces pombe]

See identical proteins and their annotated locations for NP_596488.1

Status: PROVISIONAL

UniProtKB/Swiss-Prot

O74239, Q9UUA2

Conserved Domains (3) summary

COG0507
Location:58 → 776

RecD; ATP-dependent exoDNAse (exonuclease V), alpha subunit, helicase superfamily I [Replication, recombination and repair]

pfam02689
Location:718 → 769

Herpes_Helicase; Helicase

cl21455
Location:310 → 617

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases