VAC8 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: VAC8
Primary source: SGD:S000000739
Locus tag: YEL013W
See related: AllianceGenome:SGD:S000000739; FungiDB:YEL013W; VEuPathDB:YEL013W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: YEB3
Summary: Enables protein-membrane adaptor activity. Involved in several processes, including Cvt vesicle assembly; macroautophagy; and vacuole organization. Acts upstream of or within establishment of organelle localization and protein localization to phagophore assembly site. Located in several cellular components, including fungal-type vacuole membrane; nucleus-vacuole junction; and phagophore assembly site. Is integral component of membrane. Part of Myo2p-Vac17p-Vac8p transport complex. Human ortholog(s) of this gene implicated in DiGeorge syndrome. Orthologous to human ARVCF (ARVCF delta catenin family member). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: V

Exon count:: 1

Sequence:: Chromosome: V; NC_001137.3 (128825..130561)

Chromosome V - NC_001137.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001137.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The Atg1 complex, Atg9, and Vac8 recruit PI3K complex I to the pre-autophagosomal structure.
Title: The Atg1 complex, Atg9, and Vac8 recruit PI3K complex I to the pre-autophagosomal structure.
Structures of Vac8-containing protein complexes reveal the underlying mechanism by which Vac8 regulates multiple cellular processes.
Title: Structures of Vac8-containing protein complexes reveal the underlying mechanism by which Vac8 regulates multiple cellular processes.
Vac8 determines phagophore assembly site vacuolar localization during nitrogen starvation-induced autophagy.
Title: Vac8 determines phagophore assembly site vacuolar localization during nitrogen starvation-induced autophagy.
Quaternary structures of Vac8 differentially regulate the Cvt and PMN pathways.
Title: Quaternary structures of Vac8 differentially regulate the Cvt and PMN pathways.
Authors show that Vac8 constitutes a vacuolar tether that stably anchors the PAS to the vacuole throughout autophagosome biogenesis via the PAS component Atg13. S. cerevisiae lacking Vac8 show inefficient autophagosome-vacuole fusion, and form fewer and smaller autophagosomes that often localize away from the vacuole.
Title: Vac8 spatially confines autophagosome formation at the vacuole in S. cerevisiae.
Study found that non-selective bulk autophagy of cytosolic proteins as well as the selective autophagic degradation of peroxisomes (pexophagy) and ribosomes (ribophagy) was dependent on the armadillo repeat protein Vac8 in Saccharomyces cerevisiae. Moreover, pexophagy and ribophagy depended on the palmitoylation of Vac8.
Title: Vac8 Controls Vacuolar Membrane Dynamics during Different Autophagy Pathways in Saccharomyces cerevisiae.
Report the crystal structure of Vac8p bound to Nvj1p at 2.4-A resolution. Vac8p comprises a flexibly connected N-terminal H1 helix followed by 12 armadillo repeats (ARMs) that form a right-handed superhelical structure. The extended 80-A-long loop of Nvj1p specifically binds the highly conserved inner groove formed from ARM1-12 of Vac8p.
Title: Mechanistic insight into the nucleus-vacuole junction based on the Vac8p-Nvj1p crystal structure.
Data suggest that a major role of Vac8p is to spatially separate multiple functions thereby enabling vacuole inheritance to occur concurrently with other vacuolar processes.
Title: Vac8p, an armadillo repeat protein, coordinates vacuole inheritance with multiple vacuolar processes.
Here, we address the role of Vac8 palmitoylation and show that the position and number of substrate cysteines within the SH4 domain determine the vacuole localization of Vac8: stable vacuole binding of Vac8 requires two cysteines within the N-terminus.
Title: Palmitoylation determines the function of Vac8 at the yeast vacuole.
Data show that each of the three Vac8p putative palmitoylation sites, at Cys 4, 5 and 7, may serve as a palmitoylation site.
Title: Palmitoylation plays a role in targeting Vac8p to specific membrane subdomains.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables protein-membrane adaptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein-membrane adaptor activity	IEA Inferred from Electronic Annotation more info
enables protein-membrane adaptor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein-membrane adaptor activity	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in Cvt vesicle assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in autophagosome assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in autophagy	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within establishment of organelle localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in macroautophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nucleus-vacuole junction assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in nucleus-vacuole junction assembly	IEA Inferred from Electronic Annotation more info
involved_in nucleus-vacuole junction assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pexophagy	IDA Inferred from Direct Assay more info	PubMed
involved_in piecemeal microautophagy of the nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to membrane raft	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within protein localization to phagophore assembly site	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to phagophore assembly site	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within protein-containing complex localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ribophagy	IDA Inferred from Direct Assay more info	PubMed
involved_in vacuole fusion, non-autophagic	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vacuole inheritance	IBA Inferred from Biological aspect of Ancestor more info
involved_in vacuole inheritance	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of Myo2p-Vac17p-Vac8p transport complex	IDA Inferred from Direct Assay more info	PubMed
part_of Myo2p-Vac17p-Vac8p transport complex	IPI Inferred from Physical Interaction more info	PubMed
located_in cytosol	HDA more info	PubMed
located_in fungal-type vacuole membrane	HDA more info	PubMed
is_active_in fungal-type vacuole membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in fungal-type vacuole membrane	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in membrane raft	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear membrane	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus-vacuole junction	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus-vacuole junction	IPI Inferred from Physical Interaction more info	PubMed
located_in phagophore assembly site	IMP Inferred from Mutant Phenotype more info	PubMed
located_in vacuolar membrane	IEA Inferred from Electronic Annotation more info
located_in vacuole	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: protein anchor VAC8

NP_010903.3

Vacuole-specific Myo2p receptor; Myo2p-Vac17p-Vac8p transport complex subunit required for vacuolar inheritance; mediates anchoring of phosphatidylinositol 3-kinase complex I (PIK3C3-C1) to autophagosome; required with Atg13p for the vesicle closure step of the cytoplasm-to-vacuole (CVT) pathway, for homotypic vacuole-vacuole fusion and for nucleus-vacuole junction formation with Nvj1p; contains 11 armadillo (ARM) repeats; myristoylated, palmitoylated, and phosphorylated

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001137.3 Reference assembly

Range

128825..130561

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178828.3 → NP_010903.3 TPA: protein anchor VAC8 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010903.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DLN6, P39968, Q549T0

UniProtKB/TrEMBL

A6ZQT6, B3LS03, C8Z6Y7, G2WCL9, N1P5M6

Conserved Domains (2) summary

COG5064
Location:42 → 358

SRP1; Karyopherin (importin) alpha [Intracellular trafficking and secretion]

sd00043
Location:204 → 235

ARM; armadillo repeat [structural motif]