ALG14 N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase anchoring subunit ALG14 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: ALG14
Official Full Name: N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase anchoring subunit ALG14
Primary source: SGD:S000000274
Locus tag: YBR070C
See related: AllianceGenome:SGD:S000000274; FungiDB:YBR070C; VEuPathDB:YBR070C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables protein-membrane adaptor activity. Contributes to N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity. Involved in dolichol-linked oligosaccharide biosynthetic process. Located in nuclear outer membrane-endoplasmic reticulum membrane network. Is integral component of endoplasmic reticulum membrane. Part of UDP-N-acetylglucosamine transferase complex. Human ortholog(s) of this gene implicated in congenital myasthenic syndrome 15. Orthologous to human ALG14 (ALG14 UDP-N-acetylglucosaminyltransferase subunit). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See ALG14 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 1

Sequence:: Chromosome: II; NC_001134.8 (379221..379934, complement)

Chromosome II - NC_001134.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The N- and C-termini of Alg14 coordinate recruitment of catalytic Alg7 and Alg13 to the endoplasmic reticulum membrane for initiating lipid-linked oligosaccharide biosynthesis.
Title: Alg14 organizes the formation of a multiglycosyltransferase complex involved in initiation of lipid-linked oligosaccharide biosynthesis.
Alg13 and Alg14 interaction at the C terminus mediates formation of the active UDP-N-acetylglucosamine transferase complex
Title: Interaction between the C termini of Alg13 and Alg14 mediates formation of the active UDP-N-acetylglucosamine transferase complex.
Alg13-Alg14 UDP-GlcNAc transferase represents an unprecedented example of a bipartite glycosyltransferase that evolved by both fission and fusion
Title: Membrane topology of the Alg14 endoplasmic reticulum UDP-GlcNAc transferase subunit.
Alg13p associates with Alg14p to a complex forming the active transferase catalyzing the biosynthesis of GlcNAc(2)-PP-Dol
Title: Biosynthesis of lipid-linked oligosaccharides in Saccharomyces cerevisiae: Alg13p and Alg14p form a complex required for the formation of GlcNAc(2)-PP-dolichol.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
contributes_to N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
contributes_to N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein-membrane adaptor activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in dolichol-linked oligosaccharide biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in dolichol-linked oligosaccharide biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in dolichol-linked oligosaccharide biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in dolichol-linked oligosaccharide biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dolichol-linked oligosaccharide biosynthetic process	IPI Inferred from Physical Interaction more info	PubMed

Component	Evidence Code	Pubs
part_of UDP-N-acetylglucosamine transferase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of UDP-N-acetylglucosamine transferase complex	IPI Inferred from Physical Interaction more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum membrane	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in nuclear membrane	IEA Inferred from Electronic Annotation more info
located_in nuclear outer membrane-endoplasmic reticulum membrane network	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in peroxisome	HDA more info	PubMed

General protein information

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Preferred Names: N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase anchoring subunit ALG14

NP_009626.1

Component of UDP-GlcNAc transferase; required for second step of dolichyl-linked oligosaccharide synthesis; anchors catalytic subunit Alg13p to ER membrane; localizes to the peroxisome; similar to bacterial and human glycosyltransferases; both human homologs ALG13 and ALG14 are required to complement yeast alg14 mutant; mutations of human ALG14 cause congenital myasthenic syndrome

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001134.8 Reference assembly

Range

379221..379934 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178418.1 → NP_009626.1 TPA: N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase anchoring subunit ALG14 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009626.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VQ69, P38242

UniProtKB/TrEMBL

G2W975, N1P9E2

Conserved Domains (1) summary

pfam08660
Location:57 → 236

Alg14; Oligosaccharide biosynthesis protein Alg14 like