RNR4 ribonucleotide-diphosphate reductase subunit RNR4 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RNR4
Official Full Name: ribonucleotide-diphosphate reductase subunit RNR4
Primary source: SGD:S000003412
Locus tag: YGR180C
See related: AllianceGenome:SGD:S000003412; FungiDB:YGR180C; VEuPathDB:YGR180C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CRT3; PSO3
Summary: Contributes to ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor. Involved in deoxyribonucleotide biosynthetic process. Located in cytoplasm and nucleus. Part of ribonucleoside-diphosphate reductase complex. Human ortholog(s) of this gene implicated in autosomal dominant progressive external ophthalmoplegia with mitochondrial DNA deletions 5; mitochondrial DNA depletion syndrome 8a; mitochondrial DNA depletion syndrome 8b; and pancreatic cancer. Orthologous to human RRM2B (ribonucleotide reductase regulatory TP53 inducible subunit M2B) and RRM2 (ribonucleotide reductase regulatory subunit M2). [provided by Alliance of Genome Resources, Oct 2024]
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Genomic context

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See RNR4 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (855264..856301, complement)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Rnr2-Rnr4 redistribution in S phase is triggered by Clb6-Cdc28-mediated phosphorylation of Rnr2, which disrupts the Rnr2-Wtm1 interaction and promotes the release of Rnr2-Rnr4 from the nucleus.
Title: Clb6-Cdc28 Promotes Ribonucleotide Reductase Subcellular Redistribution during S Phase.
these results reveal that, in response to iron starvation, Dun1 kinase phosphorylates Dif1 to stimulate Rnr2-Rnr4 relocalization to the cytoplasm and promote RNR function.
Title: Yeast Dun1 Kinase Regulates Ribonucleotide Reductase Small Subunit Localization in Response to Iron Deficiency.
Nearly all interactions remained unchanged or decreased after DNA damage, but 5 proteins increased interactions with Ssa1 and/or Hsp82, including the ribonucleotide reductase (RNR) subunit Rnr4.
Title: Quantitative proteomics of the yeast Hsp70/Hsp90 interactomes during DNA damage reveal chaperone-dependent regulation of ribonucleotide reductase.
diferric tyrosyl radical formation in Saccharomyces cerevisiae Rnr2 protein: requirement of Rnr4 and contribution of Grx3/4 AND Dre2 proteins.(
Title: Investigation of in vivo diferric tyrosyl radical formation in Saccharomyces cerevisiae Rnr2 protein: requirement of Rnr4 and contribution of Grx3/4 AND Dre2 proteins.
rnr4 mutants exhibit slower growth and are more sensitive to mutagens than the wild type.
Title: Low productivity of ribonucleotide reductase in Saccharomyces cerevisiae increases sensitivity to stannous chloride.
Compared to a rnr4Delta mutant that allows only very little mutation induction at very low doses of 254(nm) ultraviolet light (UVC), the pso3-1 mutant allele confers leakiness in that it permits some DNA damage-induced mutagenesis at low doses of UVC.
Title: RNR4 mutant alleles pso3-1 and rnr4Delta block induced mutation in Saccharomyces cerevisiae.
Rnr2 and Rnr4 are transported between the nucleus and the cytoplasm as one protein complex. Tagging either Rnr2 or Rnr4 with a nuclear export sequence causes cytoplasmic localization of both proteins.
Title: Cotransport of the heterodimeric small subunit of the Saccharomyces cerevisiae ribonucleotide reductase between the nucleus and the cytoplasm.
under normal growth conditions, the cell assembles stoichiometric amounts of Y(*) and modulation of Y(*) concentration is not involved in the regulation of RNR activity.
Title: Determination of the in vivo stoichiometry of tyrosyl radical per betabeta' in Saccharomyces cerevisiae ribonucleotide reductase.
studies demonstrate that beta' plays a crucial role in cluster assembly in vitro and in vivo and that the active form of the yeast R2 is betabeta'
Title: The active form of the Saccharomyces cerevisiae ribonucleotide reductase small subunit is a heterodimer in vitro and in vivo.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables oxidoreductase activity	IEA Inferred from Electronic Annotation more info
contributes_to ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor	IDA Inferred from Direct Assay more info	PubMed
enables ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor	IEA Inferred from Electronic Annotation more info
enables ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor	IGI Inferred from Genetic Interaction more info	PubMed
contributes_to ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor	IMP Inferred from Mutant Phenotype more info	PubMed
enables ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in deoxyribonucleotide biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in deoxyribonucleotide biosynthetic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	HDA more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of ribonucleoside-diphosphate reductase complex	IDA Inferred from Direct Assay more info	PubMed
part_of ribonucleoside-diphosphate reductase complex	IPI Inferred from Physical Interaction more info	PubMed

General protein information

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Preferred Names: ribonucleotide-diphosphate reductase subunit RNR4

NP_011696.3

EC 1.17.4.1

Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress; RNR4 has a paralog, RNR2, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

855264..856301 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181309.3 → NP_011696.3 TPA: ribonucleotide-diphosphate reductase subunit RNR4 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011696.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VUW4, P49723, Q66R92

UniProtKB/TrEMBL

A6ZUJ2, B3LI51, C7GT18, C8Z975, G2WER0, N1P3W2

Conserved Domains (1) summary

cl00264
Location:18 → 345

Ferritin_like; Ferritin-like superfamily of diiron-containing four-helix-bundle proteins