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    YJU2 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 853767, updated on 22-Aug-2024

    Summary

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    Official Symbol
    YJU2
    Primary source
    FungiDB:YKL095W
    Locus tag
    YKL095W
    See related
    SGD:S000001578; AllianceGenome:SGD:S000001578; VEuPathDB:YKL095W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    CWC16
    Summary
    Contributes to first spliceosomal transesterification activity. Involved in generation of catalytic spliceosome for first transesterification step. Located in nucleus. Part of U2-type catalytic step 1 spliceosome. Orthologous to human YJU2 (YJU2 splicing factor homolog). [provided by Alliance of Genome Resources, Apr 2022]
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    Genomic context

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    See YJU2 in Genome Data Viewer
    Location:
    chromosome: XI
    Exon count:
    1
    Sequence:
    Chromosome: XI; NC_001143.9 (262277..263113)

    Chromosome XI - NC_001143.9Genomic Context describing neighboring genes Neighboring gene Cwp2p Neighboring gene Cwp1p Neighboring gene acylglycerol lipase Neighboring gene Mbr1p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001143.9

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Sequence of the novel essential gene YJU2 and two flanking reading frames located within a 3.2 kb EcoRI fragment from chromosome X of Saccharomyces cerevisiae. Forrová H, et al. Yeast, 1992 May. PMID 1626433
    2. DEAH-box ATPase Prp16 has dual roles in remodeling of the spliceosome in catalytic steps. Tseng CK, et al. RNA, 2011 Jan. PMID 21098140, Free PMC Article
    3. Structure of a yeast step II catalytically activated spliceosome. Yan C, et al. Science, 2017 Jan 13. PMID 27980089
    4. Structural basis for conformational equilibrium of the catalytic spliceosome. Wilkinson ME, et al. Mol Cell, 2021 Apr 1. PMID 33705709, Free PMC Article
    5. A central role of Cwc25 in spliceosome dynamics during the catalytic phase of pre-mRNA splicing. Tseng CK, et al. RNA, 2017 Apr. PMID 28057857, Free PMC Article

    See all (41) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. In the absence of the C domain, Yju2-N domain is not stably associated with the spliceosome after lariat formation, and thus bypasses the need for Prp16.
      Title: A weak spliceosome-binding domain of Yju2 functions in the first step and bypasses Prp16 in the second step of splicing.
    2. Cwc25 functions in the final step of the first catalytic reaction after the action of Prp2.
      Title: Cwc25 is a novel splicing factor required after Prp2 and Yju2 to facilitate the first catalytic reaction.
    3. Ntc90 is required for recruiting first step factor Yju2.
      Title: Ntc90 is required for recruiting first step factor Yju2 but not for spliceosome activation.
    4. Yju2 acts in concert with an unidentified heat-resistant factor(s) in an ATP-independent manner to promote the first catalytic reaction of pre-mRNA splicing after Prp2-mediated structural rearrangement of the spliceosome.
      Title: A novel splicing factor, Yju2, is associated with NTC and acts after Prp2 in promoting the first catalytic reaction of pre-mRNA splicing.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    contributes_to first spliceosomal transesterification activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in RNA splicing IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in RNA splicing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in generation of catalytic spliceosome for first transesterification step IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in generation of catalytic spliceosome for first transesterification step IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in generation of catalytic spliceosome for second transesterification step IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in mRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in mRNA splicing, via spliceosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of U2-type catalytic step 1 spliceosome IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of U2-type catalytic step 1 spliceosome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of U2-type catalytic step 1 spliceosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of U2-type catalytic step 2 spliceosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleus HDA PubMed 
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of spliceosomal complex IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    mRNA splicing protein YJU2
    NP_012828.1
    • Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC ('nineteen complex') and acts after Prp2p to promote the first catalytic reaction of splicing

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001143.9 Reference assembly

      Range
      262277..263113
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001179661.1 → NP_012828.1  TPA: mRNA splicing protein YJU2 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_012828.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VXJ3, P28320
      UniProtKB/TrEMBL
      A6ZZM2, B3LQZ9, B5VM63, C7GVL5, C8ZC80, G2WHT8, N1P285
      Conserved Domains (1) summary
      COG5134
      Location:7 → 278
      COG5134; Uncharacterized conserved protein [Function unknown]
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P28320.1

    Gene LinkOut

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