MCA1 Ca(2+)-dependent cysteine protease MCA1 [Saccharomyces cerevisiae S288C] - Gene

Summary

Go to the top of the page Help

Official Symbol: MCA1
Official Full Name: Ca(2+)-dependent cysteine protease MCA1
Primary source: SGD:S000005723
Locus tag: YOR197W
See related: AllianceGenome:SGD:S000005723; FungiDB:YOR197W; VEuPathDB:YOR197W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: YCA1
Summary: Enables calcium-dependent cysteine-type endopeptidase activity. Involved in apoptotic process and protein quality control for misfolded or incompletely synthesized proteins. Located in cytosol and nucleus. [provided by Alliance of Genome Resources, Nov 2024]
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

See MCA1 in Genome Data Viewer

Location:: chromosome: XV

Exon count:: 1

Sequence:: Chromosome: XV; NC_001147.6 (717086..718384)

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Yca1 metacaspase: diverse functions determine how yeast live and let die.
Title: Yca1 metacaspase: diverse functions determine how yeast live and let die.
The polyunsaturated fatty acid-producing strain also showed great increase in metacaspase Yca1p activity, suggesting cells could die by caspase-mediated cell death.
Title: PUFA-induced cell death is mediated by Yca1p-dependent and -independent pathways, and is reduced by vitamin C in yeast.
The yeast metacaspase regulates both proteolytic activities through the ubiquitin-proteasome system and ceramide metabolism as revealed by proteome and metabolome profiling of YCA1-knock-out cells during acetic-acid induced programmed cell death.
Title: Proteome and metabolome profiling of wild-type and YCA1-knock-out yeast cells during acetic acid-induced programmed cell death.
Using a combination of proteomics and , it was shown that YCA1 deletion down-regulated glycolysis, the TCA cycle and alcoholic fermentation compared with WT cells.
Title: Differential proteome-metabolome profiling of YCA1-knock-out and wild type cells reveals novel metabolic pathways and cellular processes dependent on the yeast metacaspase.
Life-span extension and aggregate management by Mca1 was only partly dependent on its conserved catalytic cysteine, which suggests that Mca1 harbors both caspase-dependent and independent functions related to life-span control.
Title: Life-span extension by a metacaspase in the yeast Saccharomyces cerevisiae.
protein degradation factors such as Cdc48 co-localize with Yca1 to the insoluble fraction, supporting the hypothesis that Yca1 may act primarily to dissolve or reduce accumulated aggregates.
Title: The role of Yca1 in proteostasis. Yca1 regulates the composition of the insoluble proteome.
structural and biochemical characterization identifies Yca1 as a Ca(2+)-activated cysteine protease that may cleave specific substrates during stress response in yeast.
Title: Crystal structure of the yeast metacaspase Yca1.
Metacaspase is associated with mitochondrial respiration, intracellular redox control and genomic stability.
Title: The yeast metacaspase is implicated in oxidative stress response in frataxin-deficient cells.
the aggregation propensity of the potential yeast prion protein Mca1 in vivo is strongly accelerated by a short peptide segment preceding its Q/N-rich region
Title: Extra N-terminal residues have a profound effect on the aggregation properties of the potential yeast prion protein Mca1.
Data show that Yca1 protein complexes are enriched for aggregate-remodeling chaperones that colocalize with Yca1-GFP fusions.
Title: Metacaspase Yca1 is required for clearance of insoluble protein aggregates.

Submit: New GeneRIF Correction See all GeneRIFs (15)

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables calcium-dependent cysteine-type endopeptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables calcium-dependent cysteine-type endopeptidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables cysteine-type endopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables cysteine-type endopeptidase activity	IEA Inferred from Electronic Annotation more info
enables cysteine-type peptidase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in apoptotic process	IDA Inferred from Direct Assay more info	PubMed
involved_in apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein quality control for misfolded or incompletely synthesized proteins	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteolysis	IBA Inferred from Biological aspect of Ancestor more info
involved_in proteolysis	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

Go to the top of the page Help

Preferred Names: Ca(2+)-dependent cysteine protease MCA1

NP_014840.4

Ca2+-dependent cysteine protease; may cleave specific substrates during the stress response; regulates apoptosis upon H2O2 treatment; required for clearance of insoluble protein aggregates during normal growth; implicated in cell cycle dynamics and lifespan extension; undergoes autocatalytic processing; similar to mammalian metacaspases, but exists as a monomer due to an extra pair of anti-parallel beta-strands that block potential dimerization

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6 Reference assembly

Range

717086..718384

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183616.3 → NP_014840.4 TPA: Ca(2+)-dependent cysteine protease MCA1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014840.4

Status: REVIEWED

UniProtKB/Swiss-Prot

A6ZP43, D6W2Q6, Q08601

UniProtKB/TrEMBL

B5VS82, C7GWI2, C8ZGV6, G2WN72, N1NVU9

Conserved Domains (1) summary

pfam00656
Location:136 → 429

Peptidase_C14; Caspase domain